Experiment set3IT010 for Echinicola vietnamensis KMM 6221, DSM 17526

Compare to:

D-Mannose carbon source

Group: carbon source
Media: DinoMM_noCarbon_HighNutrient + D-Mannose (20 mM), pH=7
Culturing: Cola_ML5, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 3.7 generations
By: Adam on 8-May-17
Media components: 20 g/L Sea salts, 0.3 g/L Ammonium Sulfate, 0.1 g/L Potassium phosphate monobasic, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 1715 D1

Specific Phenotypes

For 2 genes in this experiment

For carbon source D-Mannose in Echinicola vietnamensis KMM 6221, DSM 17526

For carbon source D-Mannose across organisms

SEED Subsystems

Subsystem #Specific
L-Arabinose utilization 1
L-ascorbate utilization (and related gene clusters) 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-arabinose degradation I 4 4 1
L-lyxose degradation 4 1 1
L-ascorbate degradation I (bacterial, anaerobic) 5 1 1
L-ascorbate degradation II (bacterial, aerobic) 7 4 1
superpathway of pentose and pentitol degradation 42 11 1