Experiment set3IT006 for Pontibacter actiniarum KMM 6156, DSM 19842

marine broth with MreB Perturbing Compound A22 0.04 mg/ml

Group: stress
Media: marine_broth_2216 + MreB Perturbing Compound A22 (0.04 mg/ml)
Culturing: Ponti_ML7, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.7 generations
By: Adam on 8-May-17
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 1739 B3

Specific Phenotypes

For 65 genes in this experiment

For stress MreB Perturbing Compound A22 in Pontibacter actiniarum KMM 6156, DSM 19842

For stress MreB Perturbing Compound A22 across organisms

SEED Subsystems

Subsystem	#Specific
Heat shock dnaK gene cluster extended	2
Arginine and Ornithine Degradation	1
DNA Repair Base Excision	1
De Novo Pyrimidine Synthesis	1
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Heme and Siroheme Biosynthesis	1
Hfl operon	1
KDO2-Lipid A biosynthesis	1
MLST	1
Macromolecular synthesis operon	1
NAD and NADP cofactor biosynthesis global	1
Nitrate and nitrite ammonification	1
Nudix proteins (nucleoside triphosphate hydrolases)	1
Phosphate metabolism	1
Proline, 4-hydroxyproline uptake and utilization	1
Protein chaperones	1
Pyruvate Alanine Serine Interconversions	1
Rhamnose containing glycans	1
Universal GTPases	1
dTDP-rhamnose synthesis	1
linker unit-arabinogalactan synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glutathione metabolism
• Polyketide sugar unit biosynthesis
• Lipopolysaccharide biosynthesis
• Glycerolipid metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Glycolysis / Gluconeogenesis
• Fructose and mannose metabolism
• Purine metabolism
• Pyrimidine metabolism
• Glutamate metabolism
• Arginine and proline metabolism
• Taurine and hypotaurine metabolism
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Nucleotide sugars metabolism
• Streptomycin biosynthesis
• Keratan sulfate biosynthesis
• Peptidoglycan biosynthesis
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Reductive carboxylate cycle (CO2 fixation)
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Zeatin biosynthesis
• Nitrogen metabolism
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Flavone and flavonol biosynthesis
• Drug metabolism - other enzymes
• Biosynthesis of vancomycin group antibiotics
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UDP-α-D-galacturonate biosynthesis I (from UDP-D-glucuronate)	1	1	1
L-alanine degradation IV	1	1	1
nitrate reduction V (assimilatory)	2	1	1
alkylnitronates degradation	2	1	1
L-arginine degradation I (arginase pathway)	3	3	1
L-proline degradation I	3	2	1
glycerol degradation I	3	2	1
glycerol and glycerophosphodiester degradation	4	3	1
dTDP-6-deoxy-α-D-allose biosynthesis	4	2	1
dTDP-N-acetylthomosamine biosynthesis	4	2	1
dTDP-N-acetylviosamine biosynthesis	4	2	1
dTDP-β-D-fucofuranose biosynthesis	4	2	1
ethene biosynthesis II (microbes)	4	1	1
dTDP-β-L-rhamnose biosynthesis	5	5	1
dTDP-4-O-demethyl-β-L-noviose biosynthesis	5	4	1
trans-4-hydroxy-L-proline degradation I	5	4	1
dTDP-α-D-mycaminose biosynthesis	5	3	1
dTDP-3-acetamido-α-D-fucose biosynthesis	5	3	1
dTDP-3-acetamido-3,6-dideoxy-α-D-glucose biosynthesis	5	3	1
UMP biosynthesis II	6	6	1
UMP biosynthesis I	6	5	1
UMP biosynthesis III	6	5	1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis	6	4	1
dTDP-α-D-ravidosamine and dTDP-4-acetyl-α-D-ravidosamine biosynthesis	6	3	1
dTDP-sibirosamine biosynthesis	6	3	1
dTDP-L-daunosamine biosynthesis	6	3	1
dTDP-D-desosamine biosynthesis	6	2	1
dTDP-β-L-olivose biosynthesis	7	3	1
dTDP-β-L-digitoxose biosynthesis	7	3	1
dTDP-β-L-mycarose biosynthesis	7	2	1
L-ascorbate biosynthesis V (euglena, D-galacturonate pathway)	7	2	1
dTDP-β-L-4-epi-vancosamine biosynthesis	8	3	1
dTDP-β-L-megosamine biosynthesis	8	3	1
superpathway of pyrimidine ribonucleotides de novo biosynthesis	9	8	1
phenylacetate degradation I (aerobic)	9	6	1
UDP-sugars interconversion	9	4	1
dTDP-α-D-olivose, dTDP-α-D-oliose and dTDP-α-D-mycarose biosynthesis	9	3	1
dTDP-α-D-forosamine biosynthesis	9	3	1
superpathway of enterobacterial common antigen biosynthesis	10	2	1
O-antigen building blocks biosynthesis (E. coli)	11	8	1
superpathway of phenylethylamine degradation	11	6	1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis	18	17	1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis	19	7	1
superpathway of novobiocin biosynthesis	19	4	1
superpathway of erythromycin biosynthesis	19	2	1
superpathway of megalomicin A biosynthesis	22	3	1
superpathway of dTDP-glucose-derived antibiotic building blocks biosynthesis	23	3	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	10	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	42	1