Experiment set39IT055 for Pseudomonas putida KT2440
D-Glucose carbon source pH 6
Group: pHMedia: MOPS minimal media_noCarbon + D-Glucose (10 mM), pH=6
Culturing: Putida_ML5_JBEI, 24-well plate, Aerobic, at 30 (C), shaken=200 rpm
By: Allie Pearson on 28-Sep
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 3 genes in this experiment
For pH D-Glucose in Pseudomonas putida KT2440
For pH D-Glucose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Thiamin biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glyphosate degradation II | 1 | 1 | 1 |
| thiazole component of thiamine diphosphate biosynthesis II | 7 | 4 | 1 |
| butanol and isobutanol biosynthesis (engineered) | 8 | 3 | 1 |
| superpathway of thiamine diphosphate biosynthesis II | 11 | 8 | 1 |