Experiment set39IT036 for Pseudomonas putida KT2440

MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL

Group: stress
Media: MOPS minimal media_Glucose20mM + Carbenicillin disodium salt (100 µg/mL)
Culturing: Putida_ML5_JBEI, 24-well plate, Aerobic, at 30 (C), shaken=200 rpm
By: Allie Pearson on 28-Sep
Media components: 20 mM D-Glucose, 9.5 mM Ammonium chloride, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 2 genes in this experiment

For stress Carbenicillin disodium salt in Pseudomonas putida KT2440

For stress Carbenicillin disodium salt across organisms

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation