Experiment set39IT034 for Pseudomonas putida KT2440

Compare to:

MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL

Group: stress
Media: MOPS minimal media_Glucose20mM + Chloramphenicol (25 µg/mL)
Culturing: Putida_ML5_JBEI, 24-well plate, Aerobic, at 30 (C), shaken=200 rpm
By: Allie Pearson on 28-Sep
Media components: 20 mM D-Glucose, 9.5 mM Ammonium chloride, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 4 genes in this experiment

For stress Chloramphenicol in Pseudomonas putida KT2440

For stress Chloramphenicol across organisms

SEED Subsystems

Subsystem #Specific
Multidrug Resistance Efflux Pumps 3
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon) 2

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes: