Experiment set39IT030 for Pseudomonas putida KT2440
Propiolactam nitrogen source
Group: nitrogen sourceMedia: MOPS minimal media_Glucose_noNitrogen + 2-Azetidinone (10 mM)
Culturing: Putida_ML5_JBEI, 24-well plate, Aerobic, at 30 (C), shaken=200 rpm
By: Allie Pearson on 28-Sep
Media components: 10 mM D-Glucose, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 10 genes in this experiment
For nitrogen source 2-Azetidinone in Pseudomonas putida KT2440
For nitrogen source 2-Azetidinone across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| DNA-binding regulatory proteins, strays | 1 |
| Isobutyryl-CoA to Propionyl-CoA Module | 1 |
| Polyamine Metabolism | 1 |
| Valine degradation | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Urea cycle and metabolism of amino groups
- Valine, leucine and isoleucine degradation
- Arginine and proline metabolism
- Inositol phosphate metabolism
- Propanoate metabolism
- Atrazine degradation
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| urea degradation I | 3 | 2 | 2 |
| β-alanine degradation II | 2 | 2 | 1 |
| β-alanine degradation I | 2 | 1 | 1 |
| cyanuric acid degradation II | 5 | 3 | 2 |
| cyanuric acid degradation I | 5 | 2 | 2 |
| L-citrulline degradation | 3 | 3 | 1 |
| cyanate degradation | 3 | 2 | 1 |
| superpathway of allantoin degradation in yeast | 6 | 3 | 2 |
| L-arginine degradation V (arginine deiminase pathway) | 4 | 4 | 1 |
| superpathway of atrazine degradation | 8 | 3 | 2 |
| acrylate degradation I | 5 | 3 | 1 |
| uracil degradation III | 5 | 3 | 1 |
| propanoyl-CoA degradation II | 5 | 3 | 1 |
| 2,4-dinitrotoluene degradation | 7 | 1 | 1 |
| myo-inositol degradation I | 7 | 1 | 1 |
| L-valine degradation I | 8 | 6 | 1 |
| allantoin degradation IV (anaerobic) | 9 | 2 | 1 |
| myo-, chiro- and scyllo-inositol degradation | 10 | 1 | 1 |