Experiment set38IT054 for Pseudomonas putida KT2440

R2A with Fusaric acid 480 ug/mL

Group: stress
Media: R2A + Fusaric acid (480 ug/mL)
Culturing: Putida_ML5, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Cat_Manuel on 28-Jun-22
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate

Specific Phenotypes

For 24 genes in this experiment

For stress Fusaric acid in Pseudomonas putida KT2440

For stress Fusaric acid across organisms

SEED Subsystems

Subsystem	#Specific
Transport of Iron	2
Alginate metabolism	1
Biogenesis of cytochrome c oxidases	1
Campylobacter Iron Metabolism	1
Coenzyme B12 biosynthesis	1
Copper homeostasis	1
D-gluconate and ketogluconates metabolism	1
Entner-Doudoroff Pathway	1
Experimental tye	1
Heme and Siroheme Biosynthesis	1
Iron acquisition in Vibrio	1
Lipid A modifications	1
Mannose Metabolism	1
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria	1
Multidrug Resistance Efflux Pumps	1
Respiratory dehydrogenases 1	1
Ton and Tol transport systems	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Pentose phosphate pathway
• Fructose and mannose metabolism
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Glycine, serine and threonine metabolism
• gamma-Hexachlorocyclohexane degradation
• Bisphenol A degradation
• Benzoxazinone biosynthesis
• Glycosaminoglycan degradation
• 1- and 2-Methylnaphthalene degradation
• Naphthalene and anthracene degradation
• Benzoate degradation via CoA ligation
• Ethylbenzene degradation
• Retinol metabolism
• Porphyrin and chlorophyll metabolism
• Limonene and pinene degradation
• Diterpenoid biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
D-mannose degradation I	2	1	1
D-mannose degradation II	2	1	1
β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation	3	2	1
mannitol biosynthesis	3	1	1
GDP-mannose biosynthesis	4	4	1
heme b biosynthesis II (oxygen-independent)	4	3	1
mannitol degradation II	4	2	1
glucose degradation (oxidative)	5	5	1
glucose and glucose-1-phosphate degradation	5	4	1
1,5-anhydrofructose degradation	5	2	1
superpathway of heme b biosynthesis from uroporphyrinogen-III	6	4	1
L-ascorbate biosynthesis VIII (engineered pathway)	7	5	1
β-(1,4)-mannan degradation	7	2	1
3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic)	9	3	1
peptidoglycan recycling II	10	8	1
colanic acid building blocks biosynthesis	11	9	1
peptidoglycan recycling I	14	10	1
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis	14	7	1
superpathway of bacteriochlorophyll a biosynthesis	26	6	1