Experiment set38IT047 for Pseudomonas putida KT2440

R2A with Fusaric acid 240 ug/mL

Group: stress
Media: R2A + Fusaric acid (240 ug/mL)
Culturing: Putida_ML5, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Cat_Manuel on 28-Jun-22
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate

Specific Phenotypes

For 2 genes in this experiment

For stress Fusaric acid in Pseudomonas putida KT2440

For stress Fusaric acid across organisms

SEED Subsystems

Subsystem	#Specific
One-carbon metabolism by tetrahydropterines	1
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
formaldehyde oxidation VII (THF pathway)	4	4	1
folate transformations I	13	9	1
purine nucleobases degradation I (anaerobic)	15	6	1
purine nucleobases degradation II (anaerobic)	24	16	1