Experiment set38IT014 for Escherichia coli BW25113

Compare to:

M9 with Pyridoxine HCl 20 nM and 4-Deoxypyridoxine 0.25 uM

Group: vitamin
Media: M9 + Pyridoxine HCl (20 nM) + 4-Deoxypyridoxine (0.25 uM)
Culturing: Keio_ML9a, 24-well transparent microplate; Multitron, Aerobic, at 37 (C), shaken=700 rpm
By: Jill Babor on 25-Oct-19
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride

Specific Phenotypes

For 3 genes in this experiment

For vitamin Pyridoxine HCl in Escherichia coli BW25113

For vitamin Pyridoxine HCl across organisms

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-alanine biosynthesis III 1 1 1
L-cysteine degradation IV 1 1 1
cytidylyl molybdenum cofactor sulfurylation 2 1 1
bis(guanylyl molybdopterin) cofactor sulfurylation 3 1 1
thiazole component of thiamine diphosphate biosynthesis II 7 5 2
superpathway of L-alanine biosynthesis 4 4 1
tRNA-uridine 2-thiolation (yeast mitochondria) 4 1 1
tRNA-uridine 2-thiolation (mammalian mitochondria) 4 1 1
[2Fe-2S] iron-sulfur cluster biosynthesis 10 4 2
tRNA-uridine 2-thiolation (thermophilic bacteria) 5 1 1
superpathway of thiamine diphosphate biosynthesis II 11 9 2
molybdopterin biosynthesis 6 6 1
thiazole component of thiamine diphosphate biosynthesis I 6 6 1
tRNA-uridine 2-thiolation (cytoplasmic) 8 1 1
superpathway of thiamine diphosphate biosynthesis I 10 10 1
tRNA-uridine 2-thiolation and selenation (bacteria) 11 7 1