Experiment set35IT067 for Pseudomonas putida KT2440

PUT8 MOI 10

Group: phage
Media: LB_plus_SM_buffer + PUT8 MOI 10 + Kan (50 ug/ml)
Culturing: Putida_ML5, 48 well microplate; Epoch2, Aerobic, at 30 (C), shaken=double orbital, continuous, 205cpm
By: Vivek on 10/22/21
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 9 genes in this experiment

For phage PUT8 MOI 10 in Pseudomonas putida KT2440

For phage PUT8 MOI 10 across organisms

SEED Subsystems

Subsystem	#Specific
ABC transporter dipeptide (TC 3.A.1.5.2)	1
Bacterial Chemotaxis	1
DNA-replication	1
DNA repair, bacterial MutL-MutS system	1
Experimental tye	1
Heme and Siroheme Biosynthesis	1
Multidrug Resistance Efflux Pumps	1
Ton and Tol transport systems	1
Transcription factors bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Lipopolysaccharide biosynthesis
• Folate biosynthesis
• Porphyrin and chlorophyll metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
heme b biosynthesis II (oxygen-independent)	4	3	1
superpathway of heme b biosynthesis from uroporphyrinogen-III	6	4	1
3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic)	9	3	1
superpathway of bacteriochlorophyll a biosynthesis	26	6	1