Experiment set32IT043 for Escherichia coli BW25113

Compare to:

3hr_mediaonly_rep1_lowinoculum

200 most important genes:

  gene name fitness t score description  
b2557 purL -4.9 -13.3 phosphoribosylformylglycinamidine synthase (NCBI) compare
b0523 purE -4.9 -5.2 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b3443 yrhA -4.8 -3.7 orf, hypothetical protein (VIMSS) compare
b2312 purF -4.8 -7.9 amidophosphoribosyltransferase (NCBI) compare
b3052 rfaE -4.8 -7.3 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b1865 nudB -4.5 -2.4 dATP pyrophosphohydrolase (NCBI) compare
b0053 surA -4.4 -3.0 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b4005 purD -4.1 -9.7 phosphoribosylamine--glycine ligase (NCBI) compare
b4006 purH -4.0 -12.7 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b3621 rfaC -3.6 -9.0 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b2476 purC -3.6 -3.1 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b2499 purM -3.6 -7.2 phosphoribosylaminoimidazole synthetase (NCBI) compare
b3390 aroK -3.5 -3.8 shikimate kinase I (NCBI) compare
b0522 purK -3.3 -10.7 phosphoribosylaminoimidazole carboxylase (NCBI) compare
b3620 rfaF -3.1 -8.1 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3500 gor -3.1 -3.4 glutathione reductase (NCBI) compare
b3619 rfaD -2.9 -6.8 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b0564 appY -2.8 -1.1 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b3261 fis -2.5 -3.0 DNA-binding protein Fis (NCBI) compare
b0222 lpcA -2.4 -3.7 phosphoheptose isomerase (NCBI) compare
b0630 lipB -2.4 -1.6 protein of lipoate biosynthesis (VIMSS) compare
b4651 yibW -2.3 -1.6 no description compare
b0688 pgm -2.2 -4.1 phosphoglucomutase (NCBI) compare
b2699 recA -2.1 -1.5 recombinase A (NCBI) compare
b3183 obgE -2.0 -1.8 GTPase involved in cell partioning and DNA repair (NCBI) compare
b1863 ruvC -1.9 -2.2 Holliday junction resolvase (NCBI) compare
b4375 prfC -1.8 -5.9 peptide chain release factor 3 (NCBI) compare
b2620 smpB -1.8 -1.5 SsrA-binding protein (NCBI) compare
b3916 pfkA -1.8 -3.4 6-phosphofructokinase (NCBI) compare
b1346 ydaQ -1.8 -1.4 orf, hypothetical protein (VIMSS) compare
b0990 cspG -1.8 -1.5 DNA-binding transcriptional regulator (NCBI) compare
b0200 gmhB -1.8 -1.2 hypothetical protein (NCBI) compare
b1292 sapC -1.7 -2.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0401 brnQ -1.7 -7.0 predicted branched chain amino acid transporter (LIV-II) (NCBI) compare
b2894 xerD -1.6 -1.1 site-specific tyrosine recombinase XerD (NCBI) compare
b2830 nudH -1.6 -1.4 dinucleoside polyphosphate hydrolase (NCBI) compare
b1686 ydiI -1.6 -1.6 hypothetical protein (NCBI) compare
b2313 cvpA -1.6 -2.7 membrane protein required for colicin V production (NCBI) compare
b1860 ruvB -1.5 -1.2 Holliday junction DNA helicase B (NCBI) compare
b3405 ompR -1.5 -3.8 osmolarity response regulator (NCBI) compare
b0741 pal -1.5 -3.9 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b0740 tolB -1.4 -3.7 translocation protein TolB precursor (NCBI) compare
b3863 polA -1.4 -1.3 DNA polymerase I (NCBI) compare
b2567 rnc -1.4 -0.9 ribonuclease III (NCBI) compare
b0386 proC -1.4 -3.9 pyrroline-5-carboxylate reductase (NCBI) compare
b4509 ylcG -1.3 -3.4 DLP12 prophage; predicted protein (NCBI) compare
b1942 fliJ -1.3 -1.1 flagellar biosynthesis chaperone (NCBI) compare
b4505 insX -1.3 -1.9 predicted IS protein (RefSeq) compare
b2569 lepA -1.3 -3.2 GTP-binding protein LepA (NCBI) compare
b4179 vacB -1.3 -5.4 putative enzyme (VIMSS) compare
b1936 intG -1.3 -1.1 predicted defective phage integrase (pseudogene) (RefSeq) compare
b3082 ygjM -1.2 -1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b1192 ldcA -1.2 -2.4 L,D-carboxypeptidase A (NCBI) compare
b1286 rnb -1.2 -1.7 exoribonuclease II (NCBI) compare
b3630 rfaP -1.2 -7.2 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b2501 ppk -1.2 -5.7 polyphosphate kinase (NCBI) compare
b0737 tolQ -1.2 -2.3 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3706 trmE -1.2 -3.6 tRNA modification GTPase (NCBI) compare
b1382 ynbE -1.2 -1.4 predicted lipoprotein (NCBI) compare
b4171 miaA -1.2 -2.6 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b2318 truA -1.2 -2.3 tRNA pseudouridine synthase A (NCBI) compare
b0142 folK -1.1 -0.6 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b1025 ycdT -1.1 -2.3 predicted diguanylate cyclase (NCBI) compare
b3613 yibP -1.1 -3.1 putative membrane protein (VIMSS) compare
b0588 fepC -1.1 -4.7 iron-enterobactin transporter subunit (NCBI) compare
b2330 yfcB -1.1 -1.3 putative adenine-specific methylase (VIMSS) compare
b0590 fepD -1.1 -4.7 iron-enterobactin transporter subunit (NCBI) compare
b1777 b1777 -1.1 -1.9 orf, hypothetical protein (VIMSS) compare
b4592 yccB -1.1 -1.8 hypothetical protein (RefSeq) compare
b3199 yrbK -1.1 -1.9 hypothetical protein (NCBI) compare
b1446 ydcY -1.1 -1.7 hypothetical protein (NCBI) compare
b3811 xerC -1.1 -1.0 site-specific tyrosine recombinase XerC (NCBI) compare
b3002 yqhA -1.1 -1.3 hypothetical protein (NCBI) compare
b2363 yfdT -1.1 -2.1 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b0767 ybhE -1.1 -3.3 6-phosphogluconolactonase (NCBI) compare
b4647 mokA -1.1 -0.8 no description compare
b2506 yfgI -1.1 -2.4 hypothetical protein (NCBI) compare
b4345 mcrC -1.1 -0.7 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b3026 qseC -1.0 -2.8 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b2652 ileY -1.0 -0.7 tRNA-Ile (NCBI) compare
b0303 ykgI -1.0 -0.7 orf, hypothetical protein (VIMSS) compare
b3778 rep -1.0 -4.4 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b1252 tonB -1.0 -2.6 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b2017 yefM -1.0 -1.2 orf, hypothetical protein (VIMSS) compare
b1129 phoQ -1.0 -1.9 sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI) compare
b0439 lon -1.0 -3.7 DNA-binding ATP-dependent protease La (NCBI) compare
b0585 fes -1.0 -4.6 enterobactin/ferric enterobactin esterase (NCBI) compare
b1848 yebG -1.0 -1.3 conserved protein regulated by LexA (NCBI) compare
b0560 nohB -1.0 -2.7 DLP12 prophage; DNA packaging protein (NCBI) compare
b1261 trpB -1.0 -2.2 tryptophan synthase subunit beta (NCBI) compare
b0738 tolR -1.0 -2.3 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0949 uup -1.0 -3.7 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b4538 yoeF -1.0 -1.0 hypothetical protein (RefSeq) compare
b1159 mcrA -1.0 -0.7 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b2188 yejM -0.9 -5.0 predicted hydrolase, inner membrane (NCBI) compare
b2479 gcvR -0.9 -2.8 transcriptional regulation of gcv operon (VIMSS) compare
b3387 dam -0.9 -5.6 DNA adenine methylase (NCBI) compare
b4679 yohP -0.9 -2.7 expressed protein (RefSeq) compare
b3741 gidA -0.9 -3.5 glucose-inhibited division protein A (NCBI) compare
b3842 rfaH -0.9 -3.2 transcriptional activator RfaH (NCBI) compare
b4685 yrbN -0.9 -2.2 expressed protein (RefSeq) compare
b4146 yjeK -0.9 -2.3 predicted lysine aminomutase (NCBI) compare
b3005 exbD -0.9 -3.4 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b1179 ycgL -0.9 -2.4 conserved protein (NCBI) compare
b0726 sucA -0.8 -2.1 alpha-ketoglutarate decarboxylase (NCBI) compare
b4042 dgkA -0.8 -1.1 diacylglycerol kinase (NCBI) compare
b3027 ygiZ -0.8 -0.5 conserved inner membrane protein (NCBI) compare
b3839 tatC -0.8 -3.1 TatABCE protein translocation system subunit (NCBI) compare
b0890 ftsK -0.8 -2.8 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b4676 yoaK -0.8 -0.4 expressed protein (RefSeq) compare
b2642 yfjW -0.8 -0.8 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b4535 yniD -0.8 -1.0 predicted protein (RefSeq) compare
b1253 yciA -0.8 -2.0 predicted hydrolase (NCBI) compare
b1918 yecS -0.8 -2.0 predicted transporter subunit: membrane component of ABC superfamily (NCBI) compare
b4203 rplI -0.8 -2.7 50S ribosomal protein L9 (NCBI) compare
b0589 fepG -0.8 -4.0 iron-enterobactin transporter subunit (NCBI) compare
b1823 cspC -0.8 -1.3 stress protein, member of the CspA-family (NCBI) compare
b1084 rne -0.8 -1.9 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b3973 birA -0.8 -2.4 biotin--protein ligase (NCBI) compare
b1765 ydjA -0.8 -0.6 predicted oxidoreductase (NCBI) compare
b0178 hlpA -0.8 -2.9 periplasmic chaperone (NCBI) compare
b0584 fepA -0.8 -4.8 iron-enterobactin outer membrane transporter (NCBI) compare
b3624 rfaZ -0.8 -1.9 lipopolysaccharide core biosynthesis protein (NCBI) compare
b0623 cspE -0.7 -1.0 cold shock protein E (NCBI) compare
b2325 yfcL -0.7 -1.4 hypothetical protein (NCBI) compare
b0617 citD -0.7 -0.8 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b2369 evgA -0.7 -2.8 DNA-binding response regulator in two-component regulatory system with EvgS (NCBI) compare
b1677 lpp -0.7 -1.2 murein lipoprotein (NCBI) compare
b0018 mokC -0.7 -1.0 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b2913 serA -0.7 -3.5 D-3-phosphoglycerate dehydrogenase (NCBI) compare
b0857 potI -0.7 -2.2 putrescine transporter subunit: membrane component of ABC superfamily (NCBI) compare
b4045 yjbJ -0.7 -1.5 predicted stress response protein (NCBI) compare
b1707 ydiV -0.7 -2.4 hypothetical protein (NCBI) compare
b1236 galU -0.7 -2.3 glucose-1-phosphate uridylyltransferase (NCBI) compare
b1850 eda -0.7 -0.9 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b4407 thiS -0.7 -1.5 sulfur carrier protein ThiS (NCBI) compare
b1802 yeaW -0.7 -2.4 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b0866 ybjQ -0.7 -0.8 hypothetical protein (NCBI) compare
b1782 mipA -0.7 -2.4 scaffolding protein for murein synthesizing machinery (NCBI) compare
b1553 ydfP -0.7 -1.5 Qin prophage; conserved protein (NCBI) compare
b4699 fnrS -0.7 -0.5 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b1620 malI -0.7 -0.9 DNA-binding transcriptional repressor (NCBI) compare
b1533 ydeD -0.7 -1.3 orf, hypothetical protein (VIMSS) compare
b2498 upp -0.7 -1.0 uracil phosphoribosyltransferase (VIMSS) compare
b1175 minD -0.7 -2.1 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b3006 exbB -0.7 -3.7 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b2518 ndk -0.7 -1.1 nucleoside diphosphate kinase (NCBI) compare
b3021 ygiT -0.6 -1.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b4388 serB -0.6 -2.8 3-phosphoserine phosphatase (NCBI) compare
b1519 tam -0.6 -1.8 trans-aconitate 2-methyltransferase (NCBI) compare
b4701 sokX -0.6 -0.7 sok-related sRNA, function unknown (RefSeq) compare
b1639 ydhA -0.6 -3.2 orf, hypothetical protein (VIMSS) compare
b3250 mreC -0.6 -0.9 cell wall structural complex MreBCD transmembrane component MreC (NCBI) compare
b4513 kdpF -0.6 -1.7 potassium ion accessory transporter subunit (NCBI) compare
b0683 fur -0.6 -0.6 ferric uptake regulator (NCBI) compare
b1693 aroD -0.6 -1.7 3-dehydroquinate dehydratase (NCBI) compare
b0908 aroA -0.6 -3.0 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b4551 yheV -0.6 -1.2 hypothetical protein (NCBI) compare
b3609 secB -0.6 -1.9 export protein SecB (NCBI) compare
b1993 cobU -0.6 -2.7 adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (NCBI) compare
b2010 dacD -0.6 -3.0 penicillin binding protein 6b (VIMSS) compare
b3742 mioC -0.6 -2.8 flavodoxin (NCBI) compare
b3181 greA -0.6 -1.9 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b0994 torT -0.6 -1.8 periplasmic sensory protein associated with the TorRS two-component regulatory system (NCBI) compare
b2820 recB -0.6 -0.4 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b0280 yagN -0.6 -2.5 CP4-6 prophage; predicted protein (NCBI) compare
b0535 fimZ -0.6 -1.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b1753 ynjA -0.6 -1.2 hypothetical protein (NCBI) compare
b4655 ythA -0.6 -0.6 expressed protein (RefSeq) compare
b2576 srmB -0.6 -1.2 ATP-dependent RNA helicase (NCBI) compare
b2340 sixA -0.6 -2.3 phosphohistidine phosphatase (NCBI) compare
b1357 ydaS -0.6 -1.1 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1185 dsbB -0.6 -1.3 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b1685 ydiH -0.6 -0.8 predicted protein (RefSeq) compare
b0714 nei -0.6 -2.1 endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI) compare
b2809 b2809 -0.6 -2.1 orf, hypothetical protein (VIMSS) compare
b3836 b3836 -0.6 -2.4 orf, hypothetical protein (VIMSS) compare
b3290 trkA -0.6 -1.1 potassium transporter peripheral membrane component (NCBI) compare
b1712 ihfA -0.6 -0.8 integration host factor subunit alpha (NCBI) compare
b0912 ihfB -0.6 -1.8 integration host factor subunit beta (NCBI) compare
b3631 rfaG -0.6 -4.5 glucosyltransferase I (NCBI) compare
b0592 fepB -0.6 -3.1 iron-enterobactin transporter subunit (NCBI) compare
b2512 yfgL -0.6 -2.0 protein assembly complex, lipoprotein component (NCBI) compare
b2189 proL -0.6 -0.9 tRNA-Pro (NCBI) compare
b2350 yfdG -0.6 -2.9 CPS-53 (KpLE1) prophage; bactoprenol-linked glucose translocase (flippase) (NCBI) compare
b1536 ydeI -0.6 -0.7 hypothetical protein (NCBI) compare
b4481 rffT -0.6 -2.3 4-alpha-L-fucosyltransferase (NCBI) compare
b1478 adhP -0.6 -1.9 alcohol dehydrogenase (VIMSS) compare
b1218 chaC -0.6 -1.9 regulatory protein for cation transport (RefSeq) compare
b0216 aspV -0.6 -2.3 tRNA-Asp (NCBI) compare
b3281 aroE -0.6 -4.0 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b3238 yhcN -0.6 -1.6 orf, hypothetical protein (VIMSS) compare
b1968 yedV -0.5 -1.5 predicted sensory kinase in two-component regulatory system with YedW (NCBI) compare
b3356 yhfA -0.5 -1.7 hypothetical protein (NCBI) compare
b0471 ybaB -0.5 -1.4 hypothetical protein (NCBI) compare
b3794 rffM -0.5 -3.0 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b3257 yhdT -0.5 -2.3 conserved inner membrane protein (NCBI) compare
b3180 yhbY -0.5 -2.4 predicted RNA-binding protein (NCBI) compare
b1323 tyrR -0.5 -2.3 DNA-binding transcriptional dual regulator, tyrosine-binding (NCBI) compare
b0853 ybjN -0.5 -2.4 predicted oxidoreductase (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in msc experiments