Experiment set32IT042 for Escherichia coli BW25113
3hr_MSC_rep2_lowinoculum
Group: mscMedia: RPMI_with_Fetal_bovine_serum_Conditioned + Mesenchymal stromal cells
Culturing: Keio_ML9a, plate, Aerobic, at 37 (C), shaken=0 rpm
By: Matt/Nikki on 27-Mar-19
Media components: 10 mg/L Glycine, 200 mg/L L-Arginine, 50 mg/L L-Asparagine, 20 mg/L L-Aspartic Acid, 65 mg/L L-Cysteine hydrochloride monohydrate, 20 mg/L L-Glutamic acid monopotassium salt monohydrate, 300 mg/L L-Glutamine, 15 mg/L L-Histidine, 20 mg/L Hydroxy-L-Proline, 50 mg/L L-Isoleucine, 50 mg/L L-Leucine, 40 mg/L L-Lysine, 15 mg/L L-Methionine, 15 mg/L L-Phenylalanine, 20 mg/L L-Proline, 30 mg/L L-Serine, 20 mg/L L-Threonine, 5 mg/L L-Tryptophan, 29 mg/L L-tyrosine disodium salt, 20 mg/L L-Valine, 0.2 mg/L biotin, 3 mg/L Choline chloride, 0.25 mg/L calcium pantothenate, 1 mg/L Folic Acid, 35 mg/L m-Inositol, 1 mg/L Nicotinamide, 1 mg/L 4-Aminobenzoic acid, 1 mg/L Pyridoxine HCl, 0.2 mg/L Riboflavin, 1 mg/L Thiamine HCl, 0.005 mg/L Cyanocobalamin, 100 mg/L Calcium nitrate tetrahydrate, 48.84 mg/L Magnesium sulfate, 400 mg/L Potassium Chloride, 2000 mg/L Sodium bicarbonate, 6000 mg/L Sodium Chloride, 800 mg/L Sodium phosphate dibasic heptahydrate, 2000 mg/L D-Glucose, 1 mg/L L-glutathione reduced, 5 mg/L Phenol red, 5 vol% Fetal bovine serum, 0.1 mg/L Lipopolysaccharide
Specific Phenotypes
For 1 genes in this experiment
For msc Mesenchymal stromal cells in Escherichia coli BW25113
For msc Mesenchymal stromal cells across organisms
Metabolic Maps
Color code by fitness: see overview map or list of maps.
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| S-methyl-5'-thioadenosine degradation II | 1 | 1 | 1 |
| adenine and adenosine salvage III | 4 | 4 | 3 |
| adenine and adenosine salvage V | 3 | 3 | 2 |
| purine ribonucleosides degradation | 6 | 6 | 3 |
| adenine and adenosine salvage I | 2 | 2 | 1 |
| adenosine nucleotides degradation II | 5 | 5 | 2 |
| purine nucleotides degradation II (aerobic) | 11 | 11 | 2 |
| nucleoside and nucleotide degradation (archaea) | 10 | 4 | 1 |
| L-methionine salvage cycle III | 11 | 5 | 1 |
| superpathway of purine nucleotide salvage | 14 | 13 | 1 |