Experiment set32IT033 for Escherichia coli BW25113

Compare to:

22hr_mediaonly_rep1_highinoculum

200 most important genes:

  gene name fitness t score description  
b2476 purC -5.5 -4.0 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b3642 pyrE -5.4 -8.1 orotate phosphoribosyltransferase (NCBI) compare
b0523 purE -5.4 -5.5 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b1062 pyrC -5.3 -12.7 dihydroorotase (NCBI) compare
b4006 purH -4.8 -16.6 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b2499 purM -4.7 -8.0 phosphoribosylaminoimidazole synthetase (NCBI) compare
b2557 purL -4.4 -17.2 phosphoribosylformylglycinamidine synthase (NCBI) compare
b2312 purF -4.3 -9.6 amidophosphoribosyltransferase (NCBI) compare
b1281 pyrF -4.3 -5.1 orotidine 5'-phosphate decarboxylase (NCBI) compare
b4005 purD -4.2 -11.1 phosphoribosylamine--glycine ligase (NCBI) compare
b4388 serB -4.1 -7.3 3-phosphoserine phosphatase (NCBI) compare
b0907 serC -4.1 -9.2 phosphoserine aminotransferase (NCBI) compare
b0945 pyrD -4.1 -13.5 dihydroorotate dehydrogenase (NCBI) compare
b0049 apaH -4.0 -2.8 diadenosinetetraphosphatase (NCBI) compare
b2913 serA -4.0 -11.5 D-3-phosphoglycerate dehydrogenase (NCBI) compare
b0033 carB -3.6 -18.7 carbamoyl-phosphate synthase large subunit (NCBI) compare
b4245 pyrB -3.6 -14.7 aspartate carbamoyltransferase catalytic subunit (NCBI) compare
b1865 nudB -3.3 -2.7 dATP pyrophosphohydrolase (NCBI) compare
b0032 carA -3.2 -7.5 carbamoyl-phosphate synthase small subunit (NCBI) compare
b2318 truA -3.1 -5.7 tRNA pseudouridine synthase A (NCBI) compare
b2168 fruK -3.1 -2.2 1-phosphofructokinase (NCBI) compare
b1863 ruvC -3.0 -2.9 Holliday junction resolvase (NCBI) compare
b0564 appY -3.0 -1.1 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b0222 lpcA -2.9 -4.3 phosphoheptose isomerase (NCBI) compare
b4647 mokA -2.6 -1.4 no description compare
b0438 clpX -2.6 -7.9 ATP-dependent protease ATP-binding subunit (NCBI) compare
b3619 rfaD -2.4 -6.0 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3621 rfaC -2.4 -8.1 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3390 aroK -2.3 -3.1 shikimate kinase I (NCBI) compare
b0990 cspG -2.3 -1.6 DNA-binding transcriptional regulator (NCBI) compare
b4042 dgkA -2.3 -2.7 diacylglycerol kinase (NCBI) compare
b1236 galU -2.3 -6.3 glucose-1-phosphate uridylyltransferase (NCBI) compare
b0437 clpP -2.3 -7.4 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b3052 rfaE -2.3 -7.7 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b0522 purK -2.2 -9.7 phosphoribosylaminoimidazole carboxylase (NCBI) compare
b0889 lrp -2.2 -3.7 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b0053 surA -2.2 -6.5 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b0688 pgm -2.2 -5.2 phosphoglucomutase (NCBI) compare
b0019 nhaA -2.1 -10.8 pH-dependent sodium/proton antiporter (NCBI) compare
b0142 folK -2.1 -0.8 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b4651 yibW -2.1 -1.3 no description compare
b0145 dksA -2.1 -5.1 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b0738 tolR -2.1 -3.9 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1235 rssB -2.0 -7.6 response regulator of RpoS (NCBI) compare
b0589 fepG -2.0 -9.9 iron-enterobactin transporter subunit (NCBI) compare
b3842 rfaH -1.9 -8.6 transcriptional activator RfaH (NCBI) compare
b3387 dam -1.9 -13.8 DNA adenine methylase (NCBI) compare
b3237 argR -1.9 -6.0 arginine repressor (NCBI) compare
b3620 rfaF -1.9 -6.0 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b3026 qseC -1.9 -5.6 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b2479 gcvR -1.8 -7.6 transcriptional regulation of gcv operon (VIMSS) compare
b0741 pal -1.8 -4.7 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b0584 fepA -1.8 -10.4 iron-enterobactin outer membrane transporter (NCBI) compare
b3631 rfaG -1.8 -13.3 glucosyltransferase I (NCBI) compare
b0590 fepD -1.8 -9.6 iron-enterobactin transporter subunit (NCBI) compare
b0592 fepB -1.7 -8.5 iron-enterobactin transporter subunit (NCBI) compare
b2567 rnc -1.7 -1.0 ribonuclease III (NCBI) compare
b0588 fepC -1.7 -6.0 iron-enterobactin transporter subunit (NCBI) compare
b2504 yfgG -1.7 -2.1 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b0585 fes -1.7 -5.3 enterobactin/ferric enterobactin esterase (NCBI) compare
b0740 tolB -1.7 -4.9 translocation protein TolB precursor (NCBI) compare
b2528 iscA -1.7 -2.0 iron-sulfur cluster assembly protein (NCBI) compare
b2784 relA -1.7 -9.7 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b0912 ihfB -1.6 -4.4 integration host factor subunit beta (NCBI) compare
b0739 tolA -1.6 -3.6 cell envelope integrity inner membrane protein TolA (NCBI) compare
b3792 wzxE -1.6 -7.5 O-antigen translocase (NCBI) compare
b1712 ihfA -1.6 -1.8 integration host factor subunit alpha (NCBI) compare
b0677 nagA -1.6 -5.8 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b2313 cvpA -1.6 -3.4 membrane protein required for colicin V production (NCBI) compare
b3389 aroB -1.6 -9.0 3-dehydroquinate synthase (NCBI) compare
b3977 tyrU -1.6 -0.9 tRNA-Tyr (NCBI) compare
b2329 aroC -1.6 -5.6 chorismate synthase (NCBI) compare
b3807 cyaY -1.5 -7.0 frataxin-like protein (NCBI) compare
b3613 yibP -1.5 -3.9 putative membrane protein (VIMSS) compare
b0002 thrA -1.5 -11.3 bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI) compare
b0004 thrC -1.5 -7.8 threonine synthase (NCBI) compare
b1730 ydjO -1.4 -1.5 predicted protein (RefSeq) compare
b0890 ftsK -1.4 -3.4 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b0003 thrB -1.4 -7.7 homoserine kinase (NCBI) compare
b1252 tonB -1.4 -3.2 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b0737 tolQ -1.4 -2.8 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1984 asnW -1.4 -1.2 tRNA-Asn (NCBI) compare
b3630 rfaP -1.4 -9.3 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b4481 rffT -1.4 -6.1 4-alpha-L-fucosyltransferase (NCBI) compare
b3082 ygjM -1.4 -2.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b4317 fimD -1.4 -12.8 outer membrane usher protein, type 1 fimbrial synthesis (NCBI) compare
b3791 rffA -1.4 -8.2 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b4314 fimA -1.4 -9.7 major type 1 subunit fimbrin (pilin) (NCBI) compare
b4316 fimC -1.4 -8.2 periplasmic chaperone, required for type 1 fimbriae (VIMSS) compare
b4312 fimB -1.4 -9.5 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b0676 nagC -1.4 -7.0 DNA-binding transcriptional dual regulator, repressor of N-acetylglucosamine (NCBI) compare
b3005 exbD -1.4 -5.6 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b3911 cpxA -1.3 -9.3 two-component sensor protein (NCBI) compare
b1257 yciE -1.3 -0.7 hypothetical protein (NCBI) compare
b4178 yjeB -1.3 -4.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b3006 exbB -1.3 -8.2 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b2683 ygaH -1.3 -4.1 predicted inner membrane protein (NCBI) compare
b2699 recA -1.3 -1.5 recombinase A (NCBI) compare
b3961 oxyR -1.3 -8.0 DNA-binding transcriptional dual regulator (NCBI) compare
b2501 ppk -1.3 -7.3 polyphosphate kinase (NCBI) compare
b3790 rffC -1.3 -4.9 TDP-fucosamine acetyltransferase (RefSeq) compare
b2830 nudH -1.3 -1.3 dinucleoside polyphosphate hydrolase (NCBI) compare
b1434 ydcN -1.3 -2.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b3281 aroE -1.2 -9.5 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b0722 sdhD -1.2 -4.4 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b4435 isrC -1.2 -1.9 unknown RNA (NCBI) compare
b3609 secB -1.2 -5.4 export protein SecB (NCBI) compare
b3787 rffD -1.2 -8.8 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b2894 xerD -1.2 -1.0 site-specific tyrosine recombinase XerD (NCBI) compare
b3838 tatB -1.2 -5.3 sec-independent translocase (NCBI) compare
b2285 nuoE -1.2 -2.0 NADH dehydrogenase subunit E (NCBI) compare
b2279 nuoK -1.2 -1.0 NADH dehydrogenase subunit K (NCBI) compare
b2188 yejM -1.2 -8.6 predicted hydrolase, inner membrane (NCBI) compare
b2350 yfdG -1.1 -5.1 CPS-53 (KpLE1) prophage; bactoprenol-linked glucose translocase (flippase) (NCBI) compare
b3916 pfkA -1.1 -2.9 6-phosphofructokinase (NCBI) compare
b1144 ymfJ -1.1 -2.8 orf, hypothetical protein (VIMSS) compare
b1545 pinQ -1.1 -0.3 Qin prophage; predicted site-specific recombinase (NCBI) compare
b3786 rffE -1.1 -8.4 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b3839 tatC -1.1 -4.9 TatABCE protein translocation system subunit (NCBI) compare
b4246 pyrL -1.1 -1.9 pyrBI operon leader peptide (NCBI) compare
b3500 gor -1.1 -1.4 glutathione reductase (NCBI) compare
b3761 trpT -1.1 -1.4 tRNA-Trp (NCBI) compare
b0481 ybaK -1.1 -2.2 hypothetical protein (NCBI) compare
b2198 ccmD -1.1 -1.1 cytochrome c biogenesis protein (NCBI) compare
b0908 aroA -1.1 -5.5 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b3794 rffM -1.1 -6.2 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b0726 sucA -1.1 -3.3 alpha-ketoglutarate decarboxylase (NCBI) compare
b0755 gpmA -1.0 -4.2 phosphoglyceromutase (NCBI) compare
b3226 yhcK -1.0 -4.3 putative FADA-type transcriptional regulator (VIMSS) compare
b1058 yceO -1.0 -0.4 hypothetical protein (NCBI) compare
b2071 yegJ -1.0 -1.9 hypothetical protein (NCBI) compare
b0560 nohB -1.0 -2.2 DLP12 prophage; DNA packaging protein (NCBI) compare
b0937 ssuE -1.0 -2.4 NAD(P)H-dependent FMN reductase (NCBI) compare
b2175 spr -1.0 -3.2 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b0439 lon -1.0 -4.0 DNA-binding ATP-dependent protease La (NCBI) compare
b3774 ilvC -1.0 -7.4 ketol-acid reductoisomerase (NCBI) compare
b0101 yacG -1.0 -2.6 zinc-binding protein (NCBI) compare
b2808 gcvA -1.0 -2.9 DNA-binding transcriptional dual regulator (NCBI) compare
b2082 ogrK -1.0 -3.2 DNA-binding transcriptional regulator prophage P2 remnant (NCBI) compare
b1642 slyA -1.0 -1.5 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b1273 yciN -1.0 -1.4 hypothetical protein (NCBI) compare
b1361 ydaW -0.9 -0.4 orf, hypothetical protein (VIMSS) compare
b0097 yacA -0.9 -2.2 orf, hypothetical protein (VIMSS) compare
b2104 thiM -0.9 -2.6 hydroxyethylthiazole kinase (NCBI) compare
b0390 aroM -0.9 -4.9 hypothetical protein (NCBI) compare
b2456 cchB -0.9 -3.8 predicted carboxysome structural protein, ethanolamine utilization protein (NCBI) compare
b3778 rep -0.9 -5.2 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b3405 ompR -0.9 -3.2 osmolarity response regulator (NCBI) compare
b0928 aspC -0.9 -4.1 aspartate aminotransferase, PLP-dependent (NCBI) compare
b0721 sdhC -0.9 -2.8 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b3771 ilvD -0.9 -6.7 dihydroxy-acid dehydratase (NCBI) compare
b2572 rseA -0.9 -3.8 anti-sigma factor (NCBI) compare
b1850 eda -0.9 -2.1 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b1192 ldcA -0.9 -2.8 L,D-carboxypeptidase A (NCBI) compare
b4025 pgi -0.9 -5.6 glucose-6-phosphate isomerase (NCBI) compare
b0632 dacA -0.9 -5.7 D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI) compare
b1999 yeeP -0.9 -3.5 putative histone (VIMSS) compare
b3860 dsbA -0.9 -5.1 periplasmic protein disulfide isomerase I (NCBI) compare
b2689 yqaA -0.9 -3.0 conserved inner membrane protein (NCBI) compare
b1921 fliZ -0.9 -1.9 orf, hypothetical protein (VIMSS) compare
b1677 lpp -0.9 -1.2 murein lipoprotein (NCBI) compare
b1530 marR -0.9 -2.6 multiple antibiotic resistance protein; repressor of mar operon (VIMSS) compare
b2500 purN -0.9 -3.9 phosphoribosylglycinamide formyltransferase (NCBI) compare
b2912 ygfA -0.9 -2.2 putative ligase (VIMSS) compare
b4232 fbp -0.9 -5.5 fructose-1,6-bisphosphatase (NCBI) compare
b0178 hlpA -0.9 -2.6 periplasmic chaperone (NCBI) compare
b4202 rpsR -0.9 -1.1 30S ribosomal protein S18 (NCBI) compare
b1693 aroD -0.9 -5.4 3-dehydroquinate dehydratase (NCBI) compare
b4503 yafF -0.8 -0.5 no description compare
b4066 yjcF -0.8 -0.5 hypothetical protein (NCBI) compare
b4451 ryhB -0.8 -1.6 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b0251 yafY -0.8 -3.2 CP4-6 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b2064 asmA -0.8 -4.9 predicted assembly protein (NCBI) compare
b3210 arcB -0.8 -4.4 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b0724 sdhB -0.8 -3.8 succinate dehydrogenase, FeS subunit (NCBI) compare
b1363 trkG -0.8 -0.5 Rac prophage; potassium transporter subunit (NCBI) compare
b3035 tolC -0.8 -4.1 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b2156 lysP -0.8 -5.0 lysine transporter (NCBI) compare
b0763 modA -0.8 -3.8 molybdate transporter subunit (NCBI) compare
b1977 asnT -0.8 -1.0 tRNA-Asn (NCBI) compare
b0723 sdhA -0.8 -5.2 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b1151 ymfO -0.8 -1.8 e14 prophage; conserved protein (RefSeq) compare
b1497 b1497 -0.8 -0.5 putative enzyme (VIMSS) compare
b1438 ydcQ -0.8 -1.5 predicted DNA-binding transcriptional regulator (RefSeq) compare
b0920 ycbC -0.8 -2.8 conserved inner membrane protein (NCBI) compare
b1951 rcsA -0.8 -1.3 DNA-binding transcriptional activator, co-regulator with RcsB (NCBI) compare
b3869 glnL -0.8 -4.0 sensory histidine kinase in two-component regulatory system with GlnG (NCBI) compare
b0436 tig -0.8 -4.6 trigger factor (NCBI) compare
b1594 dgsA -0.8 -2.3 DNA-binding transcriptional repressor (NCBI) compare
b1260 trpA -0.8 -3.9 tryptophan synthase subunit alpha (NCBI) compare
b0247 ykfG -0.8 -1.2 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b0200 gmhB -0.8 -0.9 hypothetical protein (NCBI) compare
b1383 ydbL -0.8 -2.0 orf, hypothetical protein (VIMSS) compare
b1839 yebY -0.8 -1.8 hypothetical protein (NCBI) compare
b1963 b1963 -0.8 -2.9 orf, hypothetical protein (VIMSS) compare
b1347 ydaC -0.8 -0.3 Rac prophage; predicted protein (NCBI) compare
b1090 plsX -0.7 -2.8 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b1196 ycgY -0.7 -2.2 hypothetical protein (NCBI) compare
b2553 glnB -0.7 -2.6 regulatory protein P-II for glutamine synthetase (NCBI) compare
b2481 hyfA -0.7 -2.5 hydrogenase 4 Fe-S subunit (VIMSS) compare


Specific Phenotypes

For 8 genes in this experiment