Experiment set32IT032 for Escherichia coli BW25113

Compare to:

22hr_MSC_rep2_highinoculum

200 most important genes:

  gene name fitness t score description  
b2499 purM -7.2 -7.0 phosphoribosylaminoimidazole synthetase (NCBI) compare
b2476 purC -6.2 -4.5 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b4006 purH -5.9 -17.6 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b4005 purD -5.9 -13.1 phosphoribosylamine--glycine ligase (NCBI) compare
b0523 purE -5.5 -4.8 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b0907 serC -5.2 -9.4 phosphoserine aminotransferase (NCBI) compare
b2557 purL -5.0 -13.7 phosphoribosylformylglycinamidine synthase (NCBI) compare
b4388 serB -4.9 -9.8 3-phosphoserine phosphatase (NCBI) compare
b2913 serA -4.6 -12.4 D-3-phosphoglycerate dehydrogenase (NCBI) compare
b2312 purF -4.4 -8.6 amidophosphoribosyltransferase (NCBI) compare
b1281 pyrF -3.8 -5.6 orotidine 5'-phosphate decarboxylase (NCBI) compare
b3642 pyrE -3.6 -7.2 orotate phosphoribosyltransferase (NCBI) compare
b0945 pyrD -3.6 -13.9 dihydroorotate dehydrogenase (NCBI) compare
b1062 pyrC -3.5 -11.9 dihydroorotase (NCBI) compare
b4245 pyrB -3.3 -11.9 aspartate carbamoyltransferase catalytic subunit (NCBI) compare
b0522 purK -3.1 -9.9 phosphoribosylaminoimidazole carboxylase (NCBI) compare
b0033 carB -3.0 -14.6 carbamoyl-phosphate synthase large subunit (NCBI) compare
b2168 fruK -2.9 -1.5 1-phosphofructokinase (NCBI) compare
b3620 rfaF -2.9 -8.3 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b0032 carA -2.8 -11.2 carbamoyl-phosphate synthase small subunit (NCBI) compare
b1861 ruvA -2.7 -1.8 Holliday junction DNA helicase motor protein (NCBI) compare
b2784 relA -2.7 -13.2 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b0889 lrp -2.7 -3.2 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b2318 truA -2.7 -4.9 tRNA pseudouridine synthase A (NCBI) compare
b2329 aroC -2.6 -6.9 chorismate synthase (NCBI) compare
b0049 apaH -2.6 -3.9 diadenosinetetraphosphatase (NCBI) compare
b1865 nudB -2.5 -2.7 dATP pyrophosphohydrolase (NCBI) compare
b0437 clpP -2.5 -6.6 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b4699 fnrS -2.4 -1.6 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b4647 mokA -2.3 -1.6 no description compare
b0908 aroA -2.2 -9.1 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b3619 rfaD -2.2 -4.9 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b3631 rfaG -2.2 -15.3 glucosyltransferase I (NCBI) compare
b2501 ppk -2.2 -11.6 polyphosphate kinase (NCBI) compare
b0588 fepC -2.2 -8.7 iron-enterobactin transporter subunit (NCBI) compare
b3621 rfaC -2.2 -7.0 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3500 gor -2.1 -2.5 glutathione reductase (NCBI) compare
b0145 dksA -2.1 -4.9 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b0014 dnaK -2.1 -3.3 molecular chaperone DnaK (NCBI) compare
b3026 qseC -2.1 -5.1 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b0738 tolR -2.1 -2.9 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0004 thrC -2.1 -8.8 threonine synthase (NCBI) compare
b0585 fes -2.0 -7.7 enterobactin/ferric enterobactin esterase (NCBI) compare
b0002 thrA -2.0 -13.9 bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI) compare
b2498 upp -2.0 -4.2 uracil phosphoribosyltransferase (VIMSS) compare
b0589 fepG -2.0 -9.0 iron-enterobactin transporter subunit (NCBI) compare
b0590 fepD -2.0 -10.4 iron-enterobactin transporter subunit (NCBI) compare
b0003 thrB -2.0 -10.1 homoserine kinase (NCBI) compare
b0592 fepB -1.9 -10.2 iron-enterobactin transporter subunit (NCBI) compare
b3389 aroB -1.9 -8.9 3-dehydroquinate synthase (NCBI) compare
b0438 clpX -1.9 -6.0 ATP-dependent protease ATP-binding subunit (NCBI) compare
b3169 nusA -1.8 -3.2 transcription elongation factor NusA (NCBI) compare
b0740 tolB -1.8 -4.7 translocation protein TolB precursor (NCBI) compare
b3387 dam -1.8 -11.6 DNA adenine methylase (NCBI) compare
b2699 recA -1.7 -1.6 recombinase A (NCBI) compare
b0630 lipB -1.7 -1.2 protein of lipoate biosynthesis (VIMSS) compare
b0726 sucA -1.7 -4.5 alpha-ketoglutarate decarboxylase (NCBI) compare
b1642 slyA -1.7 -2.3 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b3281 aroE -1.6 -12.2 dehydroshikimate reductase, NAD(P)-binding (NCBI) compare
b3792 wzxE -1.6 -7.7 O-antigen translocase (NCBI) compare
b1860 ruvB -1.6 -1.1 Holliday junction DNA helicase B (NCBI) compare
b4481 rffT -1.6 -7.7 4-alpha-L-fucosyltransferase (NCBI) compare
b1261 trpB -1.6 -3.7 tryptophan synthase subunit beta (NCBI) compare
b4246 pyrL -1.6 -1.8 pyrBI operon leader peptide (NCBI) compare
b2282 nuoH -1.6 -2.7 NADH dehydrogenase subunit H (NCBI) compare
b0677 nagA -1.5 -6.3 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b3911 cpxA -1.5 -9.9 two-component sensor protein (NCBI) compare
b0688 pgm -1.5 -3.3 phosphoglucomutase (NCBI) compare
b3870 glnA -1.5 -3.9 glutamine synthetase (NCBI) compare
b3630 rfaP -1.5 -9.0 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b0737 tolQ -1.5 -2.8 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1332 ynaJ -1.4 -0.9 predicted inner membrane protein (NCBI) compare
b1273 yciN -1.4 -1.8 hypothetical protein (NCBI) compare
b0928 aspC -1.4 -4.7 aspartate aminotransferase, PLP-dependent (NCBI) compare
b3052 rfaE -1.4 -4.0 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b2288 nuoA -1.4 -2.9 NADH dehydrogenase I chain A (VIMSS) compare
b3390 aroK -1.4 -2.2 shikimate kinase I (NCBI) compare
b2313 cvpA -1.4 -2.0 membrane protein required for colicin V production (NCBI) compare
b0912 ihfB -1.4 -4.7 integration host factor subunit beta (NCBI) compare
b0200 gmhB -1.3 -0.9 hypothetical protein (NCBI) compare
b0101 yacG -1.3 -3.7 zinc-binding protein (NCBI) compare
b1260 trpA -1.3 -5.3 tryptophan synthase subunit alpha (NCBI) compare
b1235 rssB -1.3 -5.7 response regulator of RpoS (NCBI) compare
b3237 argR -1.3 -4.0 arginine repressor (NCBI) compare
b1563 relE -1.3 -2.2 Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI) compare
b0584 fepA -1.3 -9.6 iron-enterobactin outer membrane transporter (NCBI) compare
b2188 yejM -1.3 -7.7 predicted hydrolase, inner membrane (NCBI) compare
b4232 fbp -1.3 -7.7 fructose-1,6-bisphosphatase (NCBI) compare
b2912 ygfA -1.3 -2.5 putative ligase (VIMSS) compare
b3790 rffC -1.3 -4.7 TDP-fucosamine acetyltransferase (RefSeq) compare
b0439 lon -1.3 -4.5 DNA-binding ATP-dependent protease La (NCBI) compare
b0739 tolA -1.3 -2.8 cell envelope integrity inner membrane protein TolA (NCBI) compare
b1103 ycfF -1.3 -2.0 orf, hypothetical protein (VIMSS) compare
b1236 galU -1.3 -3.7 glucose-1-phosphate uridylyltransferase (NCBI) compare
b0222 lpcA -1.3 -2.7 phosphoheptose isomerase (NCBI) compare
b3791 rffA -1.3 -6.5 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b1850 eda -1.3 -1.9 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b2350 yfdG -1.3 -5.7 CPS-53 (KpLE1) prophage; bactoprenol-linked glucose translocase (flippase) (NCBI) compare
b1252 tonB -1.2 -3.1 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b3794 rffM -1.2 -7.7 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b3839 tatC -1.2 -4.8 TatABCE protein translocation system subunit (NCBI) compare
b3774 ilvC -1.2 -9.8 ketol-acid reductoisomerase (NCBI) compare
b0730 mngR -1.2 -1.8 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b3778 rep -1.2 -6.1 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b4676 yoaK -1.2 -0.8 expressed protein (RefSeq) compare
b3613 yibP -1.2 -3.3 putative membrane protein (VIMSS) compare
b1693 aroD -1.2 -4.1 3-dehydroquinate dehydratase (NCBI) compare
b3786 rffE -1.2 -9.1 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b3941 metF -1.2 -9.0 5,10-methylenetetrahydrofolate reductase (NCBI) compare
b0564 appY -1.2 -0.5 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b4435 isrC -1.2 -2.1 unknown RNA (NCBI) compare
b2683 ygaH -1.2 -4.2 predicted inner membrane protein (NCBI) compare
b2175 spr -1.1 -4.1 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b1175 minD -1.1 -3.8 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b0401 brnQ -1.1 -7.7 predicted branched chain amino acid transporter (LIV-II) (NCBI) compare
b3842 rfaH -1.1 -4.0 transcriptional activator RfaH (NCBI) compare
b1262 trpC -1.1 -5.5 N-(5-phosphoribosyl)anthranilate isomerase and indole-3-glycerolphosphate synthetase (VIMSS) compare
b0723 sdhA -1.1 -7.3 succinate dehydrogenase flavoprotein subunit (NCBI) compare
b3771 ilvD -1.1 -7.9 dihydroxy-acid dehydratase (NCBI) compare
b3787 rffD -1.1 -7.3 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b0676 nagC -1.1 -6.7 DNA-binding transcriptional dual regulator, repressor of N-acetylglucosamine (NCBI) compare
b2479 gcvR -1.1 -3.7 transcriptional regulation of gcv operon (VIMSS) compare
b1438 ydcQ -1.1 -2.7 predicted DNA-binding transcriptional regulator (RefSeq) compare
b2330 yfcB -1.1 -2.0 putative adenine-specific methylase (VIMSS) compare
b4312 fimB -1.1 -7.2 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b3199 yrbK -1.1 -3.0 hypothetical protein (NCBI) compare
b3027 ygiZ -1.1 -0.7 conserved inner membrane protein (NCBI) compare
b1048 mdoG -1.1 -5.1 glucan biosynthesis protein, periplasmic (NCBI) compare
b4317 fimD -1.1 -9.6 outer membrane usher protein, type 1 fimbrial synthesis (NCBI) compare
b1351 racC -1.1 -0.7 Rac prophage; predicted protein (NCBI) compare
b2569 lepA -1.1 -2.6 GTP-binding protein LepA (NCBI) compare
b2277 nuoM -1.0 -4.5 NADH dehydrogenase subunit M (NCBI) compare
b0721 sdhC -1.0 -5.2 succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI) compare
b2894 xerD -1.0 -0.7 site-specific tyrosine recombinase XerD (NCBI) compare
b1183 umuD -1.0 -2.4 DNA polymerase V, subunit D (NCBI) compare
b2276 nuoN -1.0 -4.1 NADH:ubiquinone oxidoreductase, membrane subunit N (RefSeq) compare
b3807 cyaY -1.0 -5.4 frataxin-like protein (NCBI) compare
b3236 mdh -1.0 -6.3 malate dehydrogenase (NCBI) compare
b3844 fre -1.0 -3.9 NAD(P)H-flavin reductase (NCBI) compare
b0724 sdhB -1.0 -4.6 succinate dehydrogenase, FeS subunit (NCBI) compare
b0728 sucC -1.0 -5.0 succinyl-CoA synthetase subunit beta (NCBI) compare
b3761 trpT -1.0 -1.4 tRNA-Trp (NCBI) compare
b4451 ryhB -1.0 -1.8 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b4042 dgkA -1.0 -1.3 diacylglycerol kinase (NCBI) compare
b3035 tolC -1.0 -5.6 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b0920 ycbC -1.0 -4.6 conserved inner membrane protein (NCBI) compare
b4314 fimA -1.0 -4.8 major type 1 subunit fimbrin (pilin) (NCBI) compare
b4416 rybA -1.0 -1.4 unknown RNA (NCBI) compare
b0232 yafN -1.0 -0.5 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b1677 lpp -1.0 -1.8 murein lipoprotein (NCBI) compare
b3649 rpoZ -1.0 -3.4 DNA-directed RNA polymerase subunit omega (NCBI) compare
b4048 yjbM -1.0 -1.3 hypothetical protein (NCBI) compare
b2516 yfgA -1.0 -4.8 hypothetical protein (NCBI) compare
b1040 csgD -1.0 -1.6 DNA-binding transcriptional activator in two-component regulatory system (NCBI) compare
b0720 gltA -1.0 -5.6 citrate synthase (NCBI) compare
b2897 ygfY -1.0 -2.0 hypothetical protein (NCBI) compare
b2284 nuoF -0.9 -2.6 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b3609 secB -0.9 -3.5 export protein SecB (NCBI) compare
b2500 purN -0.9 -4.5 phosphoribosylglycinamide formyltransferase (NCBI) compare
b2278 nuoL -0.9 -4.6 NADH dehydrogenase subunit L (NCBI) compare
b4179 vacB -0.9 -4.3 putative enzyme (VIMSS) compare
b0436 tig -0.9 -5.1 trigger factor (NCBI) compare
b3414 gntY -0.9 -3.3 predicted gluconate transport associated protein (NCBI) compare
b3838 tatB -0.9 -4.1 sec-independent translocase (NCBI) compare
b1160 elbA -0.9 -1.2 hypothetical protein (NCBI) compare
b0722 sdhD -0.9 -3.0 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b3627 rfaI -0.9 -5.3 UDP-D-galactose:(glucosyl)lipopolysaccharide- alpha-1,3-D-galactosyltransferase (NCBI) compare
b3741 gidA -0.9 -2.5 glucose-inhibited division protein A (NCBI) compare
b2600 tyrA -0.9 -5.8 fused chorismate mutase T/prephenate dehydrogenase (NCBI) compare
b3005 exbD -0.9 -3.1 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b0386 proC -0.9 -2.4 pyrroline-5-carboxylate reductase (NCBI) compare
b1279 yciS -0.9 -0.8 conserved inner membrane protein (NCBI) compare
b2599 pheA -0.9 -5.4 fused chorismate mutase P/prephenate dehydratase (NCBI) compare
b2283 nuoG -0.9 -3.8 NADH:ubiquinone oxidoreductase, chain G (RefSeq) compare
b1827 kdgR -0.9 -2.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1837 yebW -0.9 -2.8 orf, hypothetical protein (VIMSS) compare
b1712 ihfA -0.9 -1.5 integration host factor subunit alpha (NCBI) compare
b1426 ydcH -0.9 -1.3 orf, hypothetical protein (VIMSS) compare
b0542 renD -0.9 -1.8 orf, hypothetical protein (VIMSS) compare
b1231 tyrT -0.9 -0.7 tRNA-Tyr (NCBI) compare
b0015 dnaJ -0.9 -5.7 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b0729 sucD -0.8 -3.0 succinyl-CoA synthetase subunit alpha (NCBI) compare
b1258 yciF -0.8 -0.8 hypothetical protein (NCBI) compare
b4701 sokX -0.8 -0.9 sok-related sRNA, function unknown (RefSeq) compare
b1557 cspB -0.8 -1.4 Qin prophage; cold shock protein (NCBI) compare
b1961 dcm -0.8 -1.6 DNA cytosine methylase (NCBI) compare
b3405 ompR -0.8 -2.7 osmolarity response regulator (NCBI) compare
b0080 fruR -0.8 -3.9 DNA-binding transcriptional dual regulator (NCBI) compare
b1049 mdoH -0.8 -4.9 glucosyltransferase MdoH (NCBI) compare
b1855 lpxM -0.8 -3.1 lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (NCBI) compare
b2642 yfjW -0.8 -0.7 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b2287 nuoB -0.8 -2.4 NADH dehydrogenase subunit B (NCBI) compare
b1368 ynaA -0.8 -2.5 Rac prophage; predicted tail protein (RefSeq) compare
b2286 nuoC -0.8 -3.2 NADH:ubiquinone oxidoreductase, chain C,D (RefSeq) compare
b3181 greA -0.8 -3.8 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b0001 thrL -0.8 -0.9 thr operon leader peptide (NCBI) compare
b2990 hybG -0.8 -1.5 hydrogenase 2 accessory protein (NCBI) compare
b1594 dgsA -0.8 -2.2 DNA-binding transcriptional repressor (NCBI) compare
b0741 pal -0.8 -2.0 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b3784 rfe -0.8 -6.4 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare


Specific Phenotypes

For 6 genes in this experiment

For msc Mesenchymal stromal cells in Escherichia coli BW25113

For msc Mesenchymal stromal cells across organisms