Experiment set32IT023 for Escherichia coli BW25113

Compare to:

3hr_MSC_rep1_highinoculum

200 most important genes:

  gene name fitness t score description  
b0523 purE -4.3 -4.9 phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI) compare
b0142 folK -4.0 -1.5 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI) compare
b4006 purH -3.7 -15.9 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b4005 purD -3.5 -16.0 phosphoribosylamine--glycine ligase (NCBI) compare
b2312 purF -3.5 -7.5 amidophosphoribosyltransferase (NCBI) compare
b2557 purL -3.4 -13.5 phosphoribosylformylglycinamidine synthase (NCBI) compare
b2476 purC -3.2 -4.3 phosphoribosylaminoimidazole-succinocarboxamidesynthase (NCBI) compare
b2499 purM -2.7 -7.6 phosphoribosylaminoimidazole synthetase (NCBI) compare
b4651 yibW -2.4 -1.6 no description compare
b3619 rfaD -2.3 -6.4 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b4388 serB -2.2 -11.6 3-phosphoserine phosphatase (NCBI) compare
b0522 purK -1.9 -9.3 phosphoribosylaminoimidazole carboxylase (NCBI) compare
b2913 serA -1.8 -9.4 D-3-phosphoglycerate dehydrogenase (NCBI) compare
b2830 nudH -1.7 -2.0 dinucleoside polyphosphate hydrolase (NCBI) compare
b3621 rfaC -1.7 -7.4 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3341 rpsG -1.7 -2.1 30S ribosomal protein S7 (NCBI) compare
b2934 cmtB -1.6 -1.6 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b4245 pyrB -1.6 -10.2 aspartate carbamoyltransferase catalytic subunit (NCBI) compare
b1861 ruvA -1.6 -1.8 Holliday junction DNA helicase motor protein (NCBI) compare
b2567 rnc -1.6 -1.1 ribonuclease III (NCBI) compare
b3642 pyrE -1.6 -5.4 orotate phosphoribosyltransferase (NCBI) compare
b3052 rfaE -1.6 -5.2 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b2784 relA -1.6 -10.1 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b1062 pyrC -1.6 -9.5 dihydroorotase (NCBI) compare
b0564 appY -1.6 -1.4 DLP12 prophage; DNA-binding transcriptional activator (NCBI) compare
b3863 polA -1.5 -1.4 DNA polymerase I (NCBI) compare
b1281 pyrF -1.5 -3.9 orotidine 5'-phosphate decarboxylase (NCBI) compare
b1865 nudB -1.5 -2.3 dATP pyrophosphohydrolase (NCBI) compare
b0945 pyrD -1.5 -8.5 dihydroorotate dehydrogenase (NCBI) compare
b0033 carB -1.4 -11.6 carbamoyl-phosphate synthase large subunit (NCBI) compare
b0032 carA -1.3 -6.9 carbamoyl-phosphate synthase small subunit (NCBI) compare
b0907 serC -1.3 -7.0 phosphoserine aminotransferase (NCBI) compare
b0401 brnQ -1.3 -9.2 predicted branched chain amino acid transporter (LIV-II) (NCBI) compare
b2313 cvpA -1.3 -2.3 membrane protein required for colicin V production (NCBI) compare
b2894 xerD -1.2 -1.2 site-specific tyrosine recombinase XerD (NCBI) compare
b0889 lrp -1.2 -3.1 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b1863 ruvC -1.2 -1.7 Holliday junction resolvase (NCBI) compare
b3916 pfkA -1.2 -3.1 6-phosphofructokinase (NCBI) compare
b3620 rfaF -1.2 -4.5 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b1291 sapD -1.1 -1.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1709 btuD -1.1 -1.2 vitamin B12-transporter ATPase (NCBI) compare
b3741 gidA -1.1 -4.0 glucose-inhibited division protein A (NCBI) compare
b3706 trmE -1.0 -3.7 tRNA modification GTPase (NCBI) compare
b4676 yoaK -1.0 -0.7 expressed protein (RefSeq) compare
b2528 iscA -1.0 -1.5 iron-sulfur cluster assembly protein (NCBI) compare
b4171 miaA -1.0 -2.5 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b0737 tolQ -1.0 -2.4 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b1506 yneL -1.0 -1.7 predicted transcriptional regulator (NCBI) compare
b1257 yciE -1.0 -0.6 hypothetical protein (NCBI) compare
b3778 rep -1.0 -5.2 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b3842 rfaH -0.9 -4.5 transcriptional activator RfaH (NCBI) compare
b3405 ompR -0.9 -3.7 osmolarity response regulator (NCBI) compare
b2501 ppk -0.9 -5.5 polyphosphate kinase (NCBI) compare
b0145 dksA -0.9 -2.3 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b3811 xerC -0.9 -1.4 site-specific tyrosine recombinase XerC (NCBI) compare
b1954 dsrA -0.9 -2.6 regulatory antisense RNA (NCBI) compare
b3631 rfaG -0.9 -7.4 glucosyltransferase I (NCBI) compare
b1258 yciF -0.9 -0.8 hypothetical protein (NCBI) compare
b0053 surA -0.9 -3.3 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b3082 ygjM -0.9 -1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b2318 truA -0.9 -3.0 tRNA pseudouridine synthase A (NCBI) compare
b0738 tolR -0.9 -2.0 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b0222 lpcA -0.8 -1.8 phosphoheptose isomerase (NCBI) compare
b3026 qseC -0.8 -3.2 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b4481 rffT -0.8 -4.4 4-alpha-L-fucosyltransferase (NCBI) compare
b1882 cheY -0.8 -0.5 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b3630 rfaP -0.8 -6.2 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b4647 mokA -0.8 -0.7 no description compare
b0949 uup -0.8 -4.8 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b2168 fruK -0.8 -1.1 1-phosphofructokinase (NCBI) compare
b3839 tatC -0.8 -4.1 TatABCE protein translocation system subunit (NCBI) compare
b3387 dam -0.8 -6.2 DNA adenine methylase (NCBI) compare
b3500 gor -0.8 -1.6 glutathione reductase (NCBI) compare
b3169 nusA -0.8 -2.9 transcription elongation factor NusA (NCBI) compare
b3238 yhcN -0.8 -2.5 orf, hypothetical protein (VIMSS) compare
b4701 sokX -0.7 -0.9 sok-related sRNA, function unknown (RefSeq) compare
b2329 aroC -0.7 -3.0 chorismate synthase (NCBI) compare
b0247 ykfG -0.7 -1.5 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b1058 yceO -0.7 -0.3 hypothetical protein (NCBI) compare
b4345 mcrC -0.7 -0.8 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b4239 treC -0.7 -1.6 trehalose-6-P hydrolase (NCBI) compare
b4042 dgkA -0.7 -1.5 diacylglycerol kinase (NCBI) compare
b3979 thrT -0.7 -0.4 tRNA-Thr (NCBI) compare
b3977 tyrU -0.7 -0.7 tRNA-Tyr (NCBI) compare
b2350 yfdG -0.7 -4.2 CPS-53 (KpLE1) prophage; bactoprenol-linked glucose translocase (flippase) (NCBI) compare
b0798 ybiA -0.7 -2.2 hypothetical protein (NCBI) compare
b0505 allA -0.7 -2.7 ureidoglycolate hydrolase (NCBI) compare
b1545 pinQ -0.7 -0.2 Qin prophage; predicted site-specific recombinase (NCBI) compare
b1279 yciS -0.7 -0.9 conserved inner membrane protein (NCBI) compare
b2579 yfiD -0.7 -1.7 pyruvate formate lyase subunit (NCBI) compare
b1252 tonB -0.7 -2.6 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b4552 yrhC -0.7 -0.6 no description compare
b4025 pgi -0.6 -4.5 glucose-6-phosphate isomerase (NCBI) compare
b4246 pyrL -0.6 -2.0 pyrBI operon leader peptide (NCBI) compare
b3973 birA -0.6 -1.5 biotin--protein ligase (NCBI) compare
b0049 apaH -0.6 -1.5 diadenosinetetraphosphatase (NCBI) compare
b3613 yibP -0.6 -2.5 putative membrane protein (VIMSS) compare
b2352 yfdI -0.6 -0.4 CPS-53 (KpLE1) prophage; predicted inner membrane protein (NCBI) compare
b2820 recB -0.6 -0.6 exonuclease V (RecBCD complex), beta subunit (NCBI) compare
b1141 ymfG -0.6 -1.5 e14 prophage; predicted excisionase (NCBI) compare
b1677 lpp -0.6 -1.1 murein lipoprotein (NCBI) compare
b2620 smpB -0.6 -0.7 SsrA-binding protein (NCBI) compare
b1236 galU -0.6 -2.0 glucose-1-phosphate uridylyltransferase (NCBI) compare
b3443 yrhA -0.6 -1.5 orf, hypothetical protein (VIMSS) compare
b3838 tatB -0.6 -3.6 sec-independent translocase (NCBI) compare
b0303 ykgI -0.6 -0.7 orf, hypothetical protein (VIMSS) compare
b1085 b1085 -0.6 -1.4 orf, hypothetical protein (VIMSS) compare
b2188 yejM -0.6 -4.0 predicted hydrolase, inner membrane (NCBI) compare
b1361 ydaW -0.6 -0.3 orf, hypothetical protein (VIMSS) compare
b1150 ymfR -0.6 -0.7 e14 prophage; predicted protein (NCBI) compare
b0502 ylbG -0.6 -2.4 no description compare
b1539 ydfG -0.6 -2.4 L-allo-threonine dehydrogenase, NAD(P)-binding (NCBI) compare
b0843 ybjH -0.6 -1.2 hypothetical protein (NCBI) compare
b1025 ycdT -0.5 -1.3 predicted diguanylate cyclase (NCBI) compare
b1159 mcrA -0.5 -0.7 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b3389 aroB -0.5 -4.1 3-dehydroquinate synthase (NCBI) compare
b0740 tolB -0.5 -1.9 translocation protein TolB precursor (NCBI) compare
b4177 purA -0.5 -0.4 adenylosuccinate synthetase (NCBI) compare
b4414 tff -0.5 -2.3 unknown RNA (NCBI) compare
b2082 ogrK -0.5 -2.3 DNA-binding transcriptional regulator prophage P2 remnant (NCBI) compare
b0688 pgm -0.5 -1.7 phosphoglucomutase (NCBI) compare
b3164 pnp -0.5 -1.4 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b4631 ykgQ -0.5 -1.2 no description compare
b3791 rffA -0.5 -3.6 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b0167 glnD -0.5 -2.7 PII uridylyl-transferase (NCBI) compare
b3792 wzxE -0.5 -3.0 O-antigen translocase (NCBI) compare
b0890 ftsK -0.5 -1.9 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b3790 rffC -0.5 -2.7 TDP-fucosamine acetyltransferase (RefSeq) compare
b2822 recC -0.5 -2.5 exonuclease V (RecBCD complex), gamma chain (NCBI) compare
b2768 ygcP -0.5 -2.5 predicted anti-terminator regulatory protein (NCBI) compare
b3786 rffE -0.5 -4.3 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b3794 rffM -0.5 -3.8 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b1942 fliJ -0.5 -0.9 flagellar biosynthesis chaperone (NCBI) compare
b0232 yafN -0.5 -0.4 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b4435 isrC -0.5 -1.0 unknown RNA (NCBI) compare
b4202 rpsR -0.5 -0.9 30S ribosomal protein S18 (NCBI) compare
b1351 racC -0.5 -0.3 Rac prophage; predicted protein (NCBI) compare
b0881 clpS -0.5 -1.2 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b3787 rffD -0.5 -4.0 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b0438 clpX -0.5 -2.5 ATP-dependent protease ATP-binding subunit (NCBI) compare
b2951 yggS -0.5 -2.6 predicted enzyme (NCBI) compare
b3911 cpxA -0.5 -3.7 two-component sensor protein (NCBI) compare
b0216 aspV -0.5 -2.6 tRNA-Asp (NCBI) compare
b1557 cspB -0.5 -1.1 Qin prophage; cold shock protein (NCBI) compare
b1730 ydjO -0.5 -0.8 predicted protein (RefSeq) compare
b4685 yrbN -0.5 -1.3 expressed protein (RefSeq) compare
b1984 asnW -0.5 -0.6 tRNA-Asn (NCBI) compare
b4179 vacB -0.5 -2.3 putative enzyme (VIMSS) compare
b0585 fes -0.5 -2.3 enterobactin/ferric enterobactin esterase (NCBI) compare
b0589 fepG -0.5 -3.1 iron-enterobactin transporter subunit (NCBI) compare
b4178 yjeB -0.5 -2.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b1765 ydjA -0.5 -0.5 predicted oxidoreductase (NCBI) compare
b3006 exbB -0.5 -3.3 membrane spanning protein in TonB-ExbB-ExbD complex (NCBI) compare
b1977 asnT -0.5 -1.6 tRNA-Asn (NCBI) compare
b1810 yoaC -0.5 -2.8 predicted protein (RefSeq) compare
b1226 narJ -0.5 -2.4 molybdenum-cofactor-assembly chaperone subunit (delta subunit) of nitrate reductase 1 (NCBI) compare
b1550 b1550 -0.5 -0.7 orf, hypothetical protein (VIMSS) compare
b1651 gloA -0.5 -1.5 glyoxalase I, Ni-dependent (NCBI) compare
b4590 ybfK -0.5 -0.5 hypothetical protein (RefSeq) compare
b1676 pykF -0.5 -1.6 pyruvate kinase (NCBI) compare
b3237 argR -0.4 -2.2 arginine repressor (NCBI) compare
b2215 ompC -0.4 -2.9 outer membrane porin protein C (NCBI) compare
b1689 b1689 -0.4 -1.8 orf, hypothetical protein (VIMSS) compare
b4451 ryhB -0.4 -0.9 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b2659 b2659 -0.4 -1.2 orf, hypothetical protein (VIMSS) compare
b1269 rluB -0.4 -1.6 23S rRNA pseudouridylate synthase (NCBI) compare
b4181 yjfI -0.4 -2.0 hypothetical protein (NCBI) compare
b4538 yoeF -0.4 -0.9 hypothetical protein (RefSeq) compare
b1409 ynbB -0.4 -0.5 predicted CDP-diglyceride synthase (NCBI) compare
b1185 dsbB -0.4 -1.1 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b4093 phnO -0.4 -1.6 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b0508 hyi -0.4 -1.8 hydroxypyruvate isomerase (NCBI) compare
b0784 moaD -0.4 -1.2 molybdopterin synthase, small subunit (NCBI) compare
b0584 fepA -0.4 -2.7 iron-enterobactin outer membrane transporter (NCBI) compare
b1165 ymgA -0.4 -0.3 hypothetical protein (NCBI) compare
b1563 relE -0.4 -1.0 Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI) compare
b0592 fepB -0.4 -3.1 iron-enterobactin transporter subunit (NCBI) compare
b2175 spr -0.4 -1.3 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b0113 pdhR -0.4 -1.9 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b0097 yacA -0.4 -1.4 orf, hypothetical protein (VIMSS) compare
b1504 ydeS -0.4 -2.0 predicted fimbrial-like adhesin protein (NCBI) compare
b2330 yfcB -0.4 -0.5 putative adenine-specific methylase (VIMSS) compare
b0908 aroA -0.4 -2.5 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) compare
b3199 yrbK -0.4 -1.3 hypothetical protein (NCBI) compare
b1789 yeaL -0.4 -1.7 conserved inner membrane protein (NCBI) compare
b4694 yagP -0.4 -1.3 no description compare
b1012 b1012 -0.4 -1.6 orf, hypothetical protein (VIMSS) compare
b0471 ybaB -0.4 -1.9 hypothetical protein (NCBI) compare
b0588 fepC -0.4 -2.8 iron-enterobactin transporter subunit (NCBI) compare
b0226 dinJ -0.4 -2.5 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b1536 ydeI -0.4 -0.6 hypothetical protein (NCBI) compare
b1044 ymdA -0.4 -2.3 hypothetical protein (NCBI) compare
b1427 rimL -0.4 -1.3 ribosomal-protein-L7/L12-serine acetyltransferase (NCBI) compare
b1553 ydfP -0.4 -0.9 Qin prophage; conserved protein (NCBI) compare
b3290 trkA -0.4 -0.8 potassium transporter peripheral membrane component (NCBI) compare
b1332 ynaJ -0.4 -0.3 predicted inner membrane protein (NCBI) compare
b3180 yhbY -0.4 -2.4 predicted RNA-binding protein (NCBI) compare
b2426 ucpA -0.4 -1.2 putative oxidoreductase (VIMSS) compare
b2569 lepA -0.4 -1.4 GTP-binding protein LepA (NCBI) compare
b3935 priA -0.4 -1.0 primosome assembly protein PriA (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in msc experiments

For msc Mesenchymal stromal cells across organisms