Experiment set31IT034 for Escherichia coli BW25113

Compare to:

RCH2_defined_1percent_glucose with Sodium Chloride 200 mM and Betaine 100 uM

200 most detrimental genes:

  gene name fitness t score description  
b3261 fis +2.1 8.0 DNA-binding protein Fis (NCBI) compare
b0232 yafN +1.9 1.5 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b3183 obgE +1.7 2.9 GTPase involved in cell partioning and DNA repair (NCBI) compare
b3643 rph +1.7 4.6 ribonuclease PH (NCBI) compare
b1709 btuD +1.4 2.2 vitamin B12-transporter ATPase (NCBI) compare
b4647 mokA +1.3 2.0 no description compare
b3052 rfaE +1.1 6.3 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b0746 valZ +1.0 2.8 tRNA-Val (NCBI) compare
b2808 gcvA +1.0 3.9 DNA-binding transcriptional dual regulator (NCBI) compare
b0018 mokC +1.0 1.5 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b0740 tolB +0.9 4.3 translocation protein TolB precursor (NCBI) compare
b1861 ruvA +0.9 0.9 Holliday junction DNA helicase motor protein (NCBI) compare
b2934 cmtB +0.9 1.0 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1524 yneH +0.9 1.2 predicted glutaminase (NCBI) compare
b4685 yrbN +0.9 3.1 expressed protein (RefSeq) compare
b0881 clpS +0.9 2.3 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b3621 rfaC +0.9 5.0 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b0059 hepA +0.9 7.4 ATP-dependent helicase HepA (NCBI) compare
b3387 dam +0.9 6.6 DNA adenine methylase (NCBI) compare
b1960 vsr +0.9 2.4 DNA mismatch endonuclease of very short patch repair (NCBI) compare
b3500 gor +0.9 1.7 glutathione reductase (NCBI) compare
b3260 dusB +0.8 2.5 tRNA-dihydrouridine synthase B (NCBI) compare
b4458 oxyS +0.8 0.7 global regulatory RNA (NCBI) compare
b3545 proK +0.8 1.1 tRNA-Pro (NCBI) compare
b3620 rfaF +0.8 4.0 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b4503 yafF +0.8 0.8 no description compare
b3890 yiiF +0.8 2.1 orf, hypothetical protein (VIMSS) compare
b1192 ldcA +0.8 3.1 L,D-carboxypeptidase A (NCBI) compare
b1523 yneG +0.7 1.9 hypothetical protein (NCBI) compare
b2471 yffB +0.7 2.8 hypothetical protein (NCBI) compare
b4702 mgtL +0.7 1.1 regulatory leader peptide for mgtA (NCBI) compare
b3181 greA +0.7 3.7 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b0912 ihfB +0.7 3.6 integration host factor subunit beta (NCBI) compare
b1144 ymfJ +0.7 2.1 orf, hypothetical protein (VIMSS) compare
b2904 gcvH +0.7 2.1 glycine cleavage system protein H (NCBI) compare
b1141 ymfG +0.7 2.6 e14 prophage; predicted excisionase (NCBI) compare
b3858 yihD +0.7 2.3 hypothetical protein (NCBI) compare
b2189 proL +0.7 1.5 tRNA-Pro (NCBI) compare
b4000 hupA +0.7 2.6 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b1278 pgpB +0.7 2.1 phosphatidylglycerophosphatase B (NCBI) compare
b1569 dicC +0.7 2.8 Qin prophage; DNA-binding transcriptional regulator for DicB (NCBI) compare
b4370 leuQ +0.7 1.2 tRNA-Leu (NCBI) compare
b1842 holE +0.7 1.6 DNA polymerase III, theta subunit (NCBI) compare
b4595 yciY +0.7 1.8 hypothetical protein (RefSeq) compare
b1253 yciA +0.7 1.8 predicted hydrolase (NCBI) compare
b4201 priB +0.7 1.5 primosomal replication protein N (NCBI) compare
b4203 rplI +0.7 2.7 50S ribosomal protein L9 (NCBI) compare
b0858 ybjO +0.7 1.9 predicted inner membrane protein (NCBI) compare
b0222 lpcA +0.7 2.3 phosphoheptose isomerase (NCBI) compare
b1961 dcm +0.6 1.2 DNA cytosine methylase (NCBI) compare
b0682 ybfN +0.6 2.2 predicted lipoprotein (NCBI) compare
b0739 tolA +0.6 2.2 cell envelope integrity inner membrane protein TolA (NCBI) compare
b2279 nuoK +0.6 1.2 NADH dehydrogenase subunit K (NCBI) compare
b0843 ybjH +0.6 2.5 hypothetical protein (NCBI) compare
b4102 phnF +0.6 2.9 predicted DNA-binding transcriptional regulator of phosphonate uptake and biodegradation (NCBI) compare
b4041 plsB +0.6 1.5 glycerol-3-phosphate acyltransferase (VIMSS) compare
b0549 ybcO +0.6 1.0 DLP12 prophage; predicted protein (NCBI) compare
b2398 yfeC +0.6 2.0 orf, hypothetical protein (VIMSS) compare
b2903 gcvP +0.6 4.9 glycine dehydrogenase (NCBI) compare
b3504 yhiS +0.6 0.6 hypothetical protein (RefSeq) compare
b2912 ygfA +0.6 2.2 putative ligase (VIMSS) compare
b0852 rimK +0.6 2.9 ribosomal protein S6 modification protein (NCBI) compare
b0862 artQ +0.6 1.8 arginine transporter subunit (NCBI) compare
b1542 ydfI +0.6 2.0 predicted mannonate dehydrogenase (NCBI) compare
b4594 ymgJ +0.6 1.9 hypothetical protein (RefSeq) compare
b1266 yciV +0.6 2.2 hypothetical protein (NCBI) compare
b0741 pal +0.6 2.3 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b0688 pgm +0.6 2.0 phosphoglucomutase (NCBI) compare
b2317 dedA +0.6 1.9 conserved inner membrane protein (NCBI) compare
b3290 trkA +0.6 1.7 potassium transporter peripheral membrane component (NCBI) compare
b1300 puuC +0.6 3.0 gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent (NCBI) compare
b0752 zitB +0.6 1.9 zinc transporter ZitB (NCBI) compare
b1931 yedK +0.6 2.1 hypothetical protein (NCBI) compare
b2597 yfiA +0.6 1.3 cold shock protein associated with 30S ribosomal subunit (NCBI) compare
b4438 cyaR +0.6 1.1 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b2990 hybG +0.6 1.7 hydrogenase 2 accessory protein (NCBI) compare
b1255 yciC +0.5 1.3 hypothetical protein (NCBI) compare
b1804 rnd +0.5 2.2 ribonuclease D (NCBI) compare
b2807 ygdD +0.5 2.6 conserved inner membrane protein (NCBI) compare
b1377 ompN +0.5 2.5 outer membrane pore protein N, non-specific (NCBI) compare
b1236 galU +0.5 2.3 glucose-1-phosphate uridylyltransferase (NCBI) compare
b2248 yfaX +0.5 1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b1628 rsxB +0.5 1.4 electron transport complex protein RnfB (NCBI) compare
b0389 yaiA +0.5 1.4 hypothetical protein (NCBI) compare
b4545 ypdJ +0.5 0.9 no description compare
b1180 ycgM +0.5 2.3 predicted isomerase/hydrolase (NCBI) compare
b1575 dicB +0.5 0.8 Qin prophage; cell division inhibition protein (NCBI) compare
b4171 miaA +0.5 1.6 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b1789 yeaL +0.5 1.9 conserved inner membrane protein (NCBI) compare
b0661 miaB +0.5 3.5 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b3977 tyrU +0.5 0.5 tRNA-Tyr (NCBI) compare
b1827 kdgR +0.5 1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b2969 yghE +0.5 2.1 predicted secretion pathway protein, L-type protein (pseudogene) (RefSeq) compare
b2684 mprA +0.5 1.9 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b2236 yfaE +0.5 1.8 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b3157 yhbT +0.5 1.8 predicted lipid carrier protein (NCBI) compare
b1085 b1085 +0.5 1.6 orf, hypothetical protein (VIMSS) compare
b3341 rpsG +0.5 0.6 30S ribosomal protein S7 (NCBI) compare
b1259 yciG +0.5 1.0 orf, hypothetical protein (VIMSS) compare
b2237 inaA +0.5 2.0 hypothetical protein (NCBI) compare
b3630 rfaP +0.5 3.4 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b2125 yehT +0.5 2.0 orf, hypothetical protein (VIMSS) compare
b1531 marA +0.5 1.3 multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS) compare
b4519 icdC +0.5 1.8 no description compare
b4656 yjhY +0.5 3.3 no description compare
b2688 gshA +0.5 2.4 glutamate--cysteine ligase (NCBI) compare
b3619 rfaD +0.5 2.1 ADP-L-glycero-D-mannoheptose-6-epimerase, NAD(P)-binding (NCBI) compare
b2175 spr +0.5 1.5 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b2217 rcsB +0.5 3.2 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b0296 ykgM +0.5 0.8 50S ribosomal protein L31 (NCBI) compare
b2618 b2618 +0.5 1.3 orf, hypothetical protein (VIMSS) compare
b4538 yoeF +0.5 0.9 hypothetical protein (RefSeq) compare
b3775 ppiC +0.5 1.8 peptidyl-prolyl cis-trans isomerase C (rotamase C) (NCBI) compare
b3626 rfaJ +0.5 2.1 UDP-D-glucose:(galactosyl)lipopolysaccharide glucosyltransferase (NCBI) compare
b4581 ybeM +0.5 1.6 no description compare
b1270 btuR +0.5 2.6 cob(I)yrinic acid a,c-diamide adenosyltransferase (NCBI) compare
b1151 ymfO +0.4 1.4 e14 prophage; conserved protein (RefSeq) compare
b2170 setB +0.4 2.0 lactose/glucose efflux system (NCBI) compare
b1247 oppF +0.4 1.4 oligopeptide transporter subunit (NCBI) compare
b1176 minC +0.4 1.6 septum formation inhibitor (NCBI) compare
b2301 yfcF +0.4 1.5 predicted enzyme (NCBI) compare
b1556 b1556 +0.4 0.8 orf, hypothetical protein (VIMSS) compare
b1412 azoR +0.4 1.5 acyl carrier protein phosphodiesterase (NCBI) compare
b3238 yhcN +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b4568 ytjA +0.4 1.0 predicted protein (RefSeq) compare
b3206 npr +0.4 1.6 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1294 sapA +0.4 2.5 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0836 yliH +0.4 1.1 hypothetical protein (NCBI) compare
b4585 sroB +0.4 0.6 Novel sRNA, function unknown (RefSeq) compare
b2951 yggS +0.4 1.6 predicted enzyme (NCBI) compare
b0737 tolQ +0.4 1.5 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3024 ygiW +0.4 1.3 hypothetical protein (NCBI) compare
b2905 gcvT +0.4 2.5 glycine cleavage system aminomethyltransferase T (NCBI) compare
b2111 yehD +0.4 1.5 predicted fimbrial-like adhesin protein (NCBI) compare
b4521 ycgI +0.4 1.6 hypothetical protein (NCBI) compare
b3787 rffD +0.4 3.0 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b0710 ybgI +0.4 1.4 conserved metal-binding protein (NCBI) compare
b4017 arpA +0.4 2.0 regulator of acetyl CoA synthetase (NCBI) compare
b4692 oweE +0.4 1.5 no description compare
b1930 yedF +0.4 1.4 hypothetical protein (NCBI) compare
b2095 gatZ +0.4 2.4 D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI) compare
b3074 ygjH +0.4 1.7 hypothetical protein (NCBI) compare
b1602 pntB +0.4 1.8 pyridine nucleotide transhydrogenase (NCBI) compare
b0560 nohB +0.4 0.5 DLP12 prophage; DNA packaging protein (NCBI) compare
b3512 GadE +0.4 1.8 acid-responsive regulator of gadA and gadBC (Katherine Huang) compare
b1109 ndh +0.4 1.4 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b1639 ydhA +0.4 2.1 orf, hypothetical protein (VIMSS) compare
b0885 aat +0.4 2.1 leucyl/phenylalanyl-tRNA--protein transferase (NCBI) compare
b4415 hokE +0.4 1.4 toxic polypeptide, small (NCBI) compare
b0866 ybjQ +0.4 0.6 hypothetical protein (NCBI) compare
b0099 mutT +0.4 0.8 nucleoside triphosphate pyrophosphohydrolase, marked preference for dGTP (NCBI) compare
b0005 yaaX +0.4 1.4 hypothetical protein (NCBI) compare
b4076 nrfG +0.4 1.5 heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG (NCBI) compare
b1581 rspA +0.4 0.7 predicted dehydratase (NCBI) compare
b1976 b1976 +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b4375 prfC +0.4 1.5 peptide chain release factor 3 (NCBI) compare
b1946 fliN +0.4 1.9 flagellar motor switch protein (NCBI) compare
b1307 pspD +0.4 0.8 peripheral inner membrane phage-shock protein (NCBI) compare
b1760 ynjH +0.4 1.4 hypothetical protein (NCBI) compare
b2792 yqcC +0.4 2.1 hypothetical protein (NCBI) compare
b0423 thiI +0.4 1.9 thiamine biosynthesis protein ThiI (NCBI) compare
b4571 wbbL +0.4 1.1 no description compare
b3998 nfi +0.4 2.4 endonuclease V (deoxyinosine 3'endoduclease) (VIMSS) compare
b3037 ygiB +0.4 1.7 conserved outer membrane protein (RefSeq) compare
b1148 ymfM +0.4 0.9 e14 prophage; predicted protein (NCBI) compare
b4451 ryhB +0.4 0.9 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b2136 yohD +0.4 1.6 orf, hypothetical protein (VIMSS) compare
b1156 tfaE +0.4 1.5 e14 prophage; predicted tail fiber assembly protein (NCBI) compare
b4408 csrB +0.4 1.3 CsrA-binding sRNA, antagonizing CsrA regulation (NCBI) compare
b2829 ptsP +0.4 2.9 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b4609 ryfD +0.4 1.2 Novel sRNA, function unknown (RefSeq) compare
b3264 envR +0.4 1.5 DNA-binding transcriptional regulator (NCBI) compare
b3889 yiiE +0.4 1.1 predicted transcriptional regulator (RefSeq) compare
b0830 yliB +0.4 1.9 predicted peptide transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b1642 slyA +0.4 0.6 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b0616 citE +0.4 1.1 citrate lyase beta chain (acyl lyase subunit) (VIMSS) compare
b3786 rffE +0.4 2.7 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b3292 zntR +0.4 1.3 zinc-responsive transcriptional regulator (NCBI) compare
b2565 recO +0.4 1.9 DNA repair protein RecO (NCBI) compare
b2778 ygcG +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b0759 galE +0.4 1.9 UDP-galactose-4-epimerase (VIMSS) compare
b1674 ydhY +0.4 1.7 predicted 4Fe-4S ferridoxin-type protein (NCBI) compare
b3670 ilvN +0.4 2.1 acetolactate synthase small subunit (NCBI) compare
b2956 yggM +0.4 1.7 hypothetical protein (NCBI) compare
b3871 bipA +0.4 2.7 GTP-binding protein (NCBI) compare
b4135 yjdC +0.4 1.9 orf, hypothetical protein (VIMSS) compare
b1336 ydaH +0.4 1.7 putative pump protein (transport) (VIMSS) compare
b3686 ibpB +0.4 2.1 heat shock chaperone (NCBI) compare
b2050 wcaI +0.4 1.5 predicted glycosyl transferase (NCBI) compare
b4606 ypfM +0.4 1.2 hypothetical protein (RefSeq) compare
b0979 appB +0.4 1.2 cytochrome bd-II oxidase, subunit II (NCBI) compare
b0137 yadL +0.4 2.3 predicted fimbrial-like adhesin protein (NCBI) compare
b4650 yibS +0.4 1.1 no description compare
b2393 nupC +0.4 1.8 nucleoside (except guanosine) transporter (NCBI) compare
b1429 tehA +0.4 0.4 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b1515 lsrD +0.4 1.3 AI2 transporter (NCBI) compare
b1728 ydjM +0.4 0.8 predicted inner membrane protein regulated by LexA (RefSeq) compare
b4104 phnE +0.4 1.4 phosphonate/organophosphate ester transporter subunit (NCBI) compare
b0210 yafE +0.4 1.1 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b0849 grxA +0.4 1.3 glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a) (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Sodium Chloride across organisms