Experiment set31IT033 for Escherichia coli BW25113

Compare to:

RCH2_defined_1percent_glucose with Sodium Chloride 100 mM and Betaine 100 uM

200 most detrimental genes:

  gene name fitness t score description  
b3261 fis +2.5 9.2 DNA-binding protein Fis (NCBI) compare
b3643 rph +1.7 4.5 ribonuclease PH (NCBI) compare
b3979 thrT +1.6 1.3 tRNA-Thr (NCBI) compare
b2808 gcvA +1.3 7.0 DNA-binding transcriptional dual regulator (NCBI) compare
b0059 hepA +1.2 11.0 ATP-dependent helicase HepA (NCBI) compare
b0630 lipB +1.1 2.0 protein of lipoate biosynthesis (VIMSS) compare
b2904 gcvH +1.1 3.6 glycine cleavage system protein H (NCBI) compare
b2189 proL +1.0 2.8 tRNA-Pro (NCBI) compare
b2903 gcvP +0.9 8.2 glycine dehydrogenase (NCBI) compare
b2951 yggS +0.9 5.6 predicted enzyme (NCBI) compare
b3387 dam +0.9 7.3 DNA adenine methylase (NCBI) compare
b4203 rplI +0.9 4.4 50S ribosomal protein L9 (NCBI) compare
b3260 dusB +0.8 2.4 tRNA-dihydrouridine synthase B (NCBI) compare
b3181 greA +0.8 4.0 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b3977 tyrU +0.8 1.2 tRNA-Tyr (NCBI) compare
b4592 yccB +0.8 2.6 hypothetical protein (RefSeq) compare
b2236 yfaE +0.7 2.8 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b2905 gcvT +0.7 4.0 glycine cleavage system aminomethyltransferase T (NCBI) compare
b0014 dnaK +0.7 1.4 molecular chaperone DnaK (NCBI) compare
b4048 yjbM +0.7 1.5 hypothetical protein (NCBI) compare
b0661 miaB +0.7 5.0 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b0881 clpS +0.7 1.9 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b2058 wcaB +0.6 1.2 predicted acyl transferase (NCBI) compare
b1569 dicC +0.6 3.0 Qin prophage; DNA-binding transcriptional regulator for DicB (NCBI) compare
b3206 npr +0.6 4.9 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1151 ymfO +0.6 1.8 e14 prophage; conserved protein (RefSeq) compare
b1480 sra +0.6 1.3 30S ribosomal subunit protein S22 (NCBI) compare
b1607 ydgC +0.6 0.9 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b4655 ythA +0.6 0.9 expressed protein (RefSeq) compare
b3844 fre +0.6 3.8 NAD(P)H-flavin reductase (NCBI) compare
b1790 yeaM +0.6 2.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b4000 hupA +0.6 3.4 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b1550 b1550 +0.6 1.4 orf, hypothetical protein (VIMSS) compare
b4375 prfC +0.6 3.0 peptide chain release factor 3 (NCBI) compare
b2597 yfiA +0.6 1.7 cold shock protein associated with 30S ribosomal subunit (NCBI) compare
b1630 rsxD +0.6 3.0 electron transport complex protein RnfD (NCBI) compare
b4171 miaA +0.6 2.4 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b1823 cspC +0.6 1.8 stress protein, member of the CspA-family (NCBI) compare
b2297 pta +0.6 0.8 phosphate acetyltransferase (NCBI) compare
b2618 b2618 +0.6 2.7 orf, hypothetical protein (VIMSS) compare
b1236 galU +0.6 3.0 glucose-1-phosphate uridylyltransferase (NCBI) compare
b4519 icdC +0.6 2.2 no description compare
b3164 pnp +0.5 2.1 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b1921 fliZ +0.5 2.4 orf, hypothetical protein (VIMSS) compare
b1253 yciA +0.5 2.7 predicted hydrolase (NCBI) compare
b4373 rimI +0.5 1.8 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b1963 b1963 +0.5 1.8 orf, hypothetical protein (VIMSS) compare
b2083 yegZ +0.5 1.2 orf, hypothetical protein (VIMSS) compare
b0866 ybjQ +0.5 1.1 hypothetical protein (NCBI) compare
b4522 yciX_1 +0.5 1.3 hypothetical protein (NCBI) compare
b0688 pgm +0.5 2.4 phosphoglucomutase (NCBI) compare
b2829 ptsP +0.5 4.3 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b3119 tdcR +0.5 1.2 threonine dehydratase operon activator protein (VIMSS) compare
b2528 iscA +0.5 1.3 iron-sulfur cluster assembly protein (NCBI) compare
b3998 nfi +0.5 3.2 endonuclease V (deoxyinosine 3'endoduclease) (VIMSS) compare
b0246 yafW +0.5 2.7 CP4-6 prophage; antitoxin of the YkfI-YafW toxin-antitoxin system (NCBI) compare
b1013 ycdC +0.5 1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b2684 mprA +0.5 2.9 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b1542 ydfI +0.5 1.5 predicted mannonate dehydrogenase (NCBI) compare
b1109 ndh +0.5 2.6 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b1575 dicB +0.5 1.1 Qin prophage; cell division inhibition protein (NCBI) compare
b0216 aspV +0.5 2.4 tRNA-Asp (NCBI) compare
b2688 gshA +0.5 3.7 glutamate--cysteine ligase (NCBI) compare
b1090 plsX +0.5 2.2 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b2741 rpoS +0.5 2.3 RNA polymerase sigma factor (NCBI) compare
b2579 yfiD +0.5 1.9 pyruvate formate lyase subunit (NCBI) compare
b0949 uup +0.5 2.2 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b4397 creA +0.5 2.3 hypothetical protein (NCBI) compare
b1804 rnd +0.5 2.9 ribonuclease D (NCBI) compare
b1085 b1085 +0.5 1.2 orf, hypothetical protein (VIMSS) compare
b2141 yohJ +0.5 1.9 hypothetical protein (NCBI) compare
b3780 rhlB +0.4 3.8 ATP-dependent RNA helicase (NCBI) compare
b0296 ykgM +0.4 1.2 50S ribosomal protein L31 (NCBI) compare
b0147 yadP +0.4 2.9 orf, hypothetical protein (VIMSS) compare
b0912 ihfB +0.4 1.5 integration host factor subunit beta (NCBI) compare
b2910 zapA +0.4 2.0 protein that localizes to the cytokinetic ring (NCBI) compare
b1518 lsrG +0.4 0.9 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b4606 ypfM +0.4 1.8 hypothetical protein (RefSeq) compare
b1539 ydfG +0.4 2.0 L-allo-threonine dehydrogenase, NAD(P)-binding (NCBI) compare
b1103 ycfF +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b1758 b1758 +0.4 0.8 putative cytochrome oxidase (VIMSS) compare
b1842 holE +0.4 1.1 DNA polymerase III, theta subunit (NCBI) compare
b4609 ryfD +0.4 1.6 Novel sRNA, function unknown (RefSeq) compare
b1412 azoR +0.4 1.6 acyl carrier protein phosphodiesterase (NCBI) compare
b0461 ybaJ +0.4 2.6 hypothetical protein (NCBI) compare
b2947 gshB +0.4 1.8 glutathione synthetase (NCBI) compare
b1144 ymfJ +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b0746 valZ +0.4 1.3 tRNA-Val (NCBI) compare
b3787 rffD +0.4 2.9 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b3555 yiaG +0.4 2.0 predicted transcriptional regulator (NCBI) compare
b0605 ahpC +0.4 1.5 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b2912 ygfA +0.4 1.3 putative ligase (VIMSS) compare
b2175 spr +0.4 1.5 predicted peptidase, outer membrane lipoprotein (NCBI) compare
b1190 dadX +0.4 1.9 alanine racemase (NCBI) compare
b1651 gloA +0.4 1.6 glyoxalase I, Ni-dependent (NCBI) compare
b2824 ygdB +0.4 1.3 orf, hypothetical protein (VIMSS) compare
b3644 yicC +0.4 2.0 hypothetical protein (NCBI) compare
b1936 intG +0.4 0.4 predicted defective phage integrase (pseudogene) (RefSeq) compare
b3858 yihD +0.4 2.0 hypothetical protein (NCBI) compare
b1531 marA +0.4 1.0 multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS) compare
b1296 ycjJ +0.4 1.9 putative amino acid/amine transport protein (VIMSS) compare
b4460 araH +0.4 0.7 fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq) compare
b1290 sapF +0.4 0.7 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1802 yeaW +0.4 1.7 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b1567 intK +0.4 1.2 Qin prophage; predicted protein (RefSeq) compare
b4445 omrB +0.4 1.9 sRNA down regulates OM proteins; positively regulated by OmpR/EnvZ; binds Hfq (RefSeq) compare
b4135 yjdC +0.4 2.1 orf, hypothetical protein (VIMSS) compare
b3670 ilvN +0.4 2.4 acetolactate synthase small subunit (NCBI) compare
b3786 rffE +0.4 3.1 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b1732 katE +0.4 2.5 hydroperoxidase HPII(III) (catalase) (NCBI) compare
b4391 yjjK +0.4 2.9 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1380 ldhA +0.4 0.9 D-lactate dehydrogenase (NCBI) compare
b0234 yafP +0.4 1.1 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b4255 yjgD +0.4 1.6 hypothetical protein (NCBI) compare
b2248 yfaX +0.4 1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b1073 flgB +0.4 1.6 flagellar basal-body rod protein B (NCBI) compare
b3706 trmE +0.4 1.3 tRNA modification GTPase (NCBI) compare
b3842 rfaH +0.4 2.2 transcriptional activator RfaH (NCBI) compare
b2159 nfo +0.4 2.0 endonuclease IV (NCBI) compare
b4675 yoaJ +0.4 1.2 expressed protein (RefSeq) compare
b4595 yciY +0.4 0.8 hypothetical protein (RefSeq) compare
b2778 ygcG +0.4 2.1 orf, hypothetical protein (VIMSS) compare
b1370 insH-5 +0.4 0.9 Rac prophage; IS5 transposase and trans-activator (NCBI) compare
b4152 frdC +0.4 2.2 fumarate reductase subunit C (NCBI) compare
b1488 ddpX +0.4 1.6 D-ala-D-ala dipeptidase, Zn-dependent (NCBI) compare
b3761 trpT +0.4 0.8 tRNA-Trp (NCBI) compare
b1138 ymfE +0.4 0.7 e14 prophage; predicted inner membrane protein (NCBI) compare
b3199 yrbK +0.4 0.6 hypothetical protein (NCBI) compare
b4453 ldrD +0.4 1.2 toxic polypeptide, small (NCBI) compare
b3794 rffM +0.4 2.6 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b1593 b1593 +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b1088 yceD +0.4 2.1 hypothetical protein (NCBI) compare
b1725 yniA +0.4 1.6 predicted phosphotransferase/kinase (NCBI) compare
b4224 chpS +0.4 1.7 suppressor of inhibitory function of ChpB, PemI-like, autoregulated (VIMSS) compare
b1821 b1821 +0.4 1.5 orf, hypothetical protein (VIMSS) compare
b1883 cheB +0.4 2.4 chemotaxis-specific methylesterase (NCBI) compare
b2217 rcsB +0.4 2.3 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b3394 yrfC +0.4 2.4 predicted fimbrial assembly protein (NCBI) compare
b1357 ydaS +0.4 1.1 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b1831 proQ +0.4 1.5 putative solute/DNA competence effector (NCBI) compare
b1222 narX +0.4 1.8 sensory histidine kinase in two-component regulatory system with NarL (NCBI) compare
b4196 ulaD +0.4 2.1 3-keto-L-gulonate 6-phosphate decarboxylase (NCBI) compare
b1827 kdgR +0.4 1.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b1153 ymfQ +0.4 1.6 e14 prophage; conserved protein (NCBI) compare
b1960 vsr +0.4 1.8 DNA mismatch endonuclease of very short patch repair (NCBI) compare
b2786 barA +0.4 3.3 hybrid sensory histidine kinase, in two-component regulatory system with UvrY (NCBI) compare
b0806 ybiM +0.4 1.0 orf, hypothetical protein (VIMSS) compare
b0550 rusA +0.4 0.8 DLP12 prophage; endonuclease RUS (NCBI) compare
b3052 rfaE +0.4 1.8 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b0195 yaeB +0.3 1.9 hypothetical protein (NCBI) compare
b1305 pspB +0.3 1.2 phage shock protein B (NCBI) compare
b2125 yehT +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b1724 ydiZ +0.3 1.0 hypothetical protein (NCBI) compare
b1760 ynjH +0.3 1.0 hypothetical protein (NCBI) compare
b3229 sspA +0.3 1.2 stringent starvation protein A (NCBI) compare
b0101 yacG +0.3 1.1 zinc-binding protein (NCBI) compare
b0227 yafL +0.3 1.4 predicted lipoprotein and C40 family peptidase (NCBI) compare
b2071 yegJ +0.3 0.7 hypothetical protein (NCBI) compare
b0503 ybbB +0.3 1.3 tRNA 2-selenouridine synthase, selenophosphate-dependent (NCBI) compare
b3740 gidB +0.3 2.7 glucose-inhibited division protein B (NCBI) compare
b2183 rsuA +0.3 1.2 16S rRNA pseudouridylate 516 synthase (NCBI) compare
b1147 ymfL +0.3 1.5 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b1590 ynfH +0.3 1.4 oxidoreductase, membrane subunit (NCBI) compare
b1329 b1329 +0.3 2.3 putative transport periplasmic protein (VIMSS) compare
b0897 ycaC +0.3 2.0 predicted hydrolase (NCBI) compare
b1400 paaY +0.3 1.2 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b4533 ynfO +0.3 1.2 Qin prophage; predicted protein (NCBI) compare
b2212 alkB +0.3 2.2 oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI) compare
b3790 rffC +0.3 1.7 TDP-fucosamine acetyltransferase (RefSeq) compare
b3141 agaI +0.3 2.3 galactosamine-6-phosphate isomerase (NCBI) compare
b0167 glnD +0.3 1.3 PII uridylyl-transferase (NCBI) compare
b1916 sdiA +0.3 2.4 DNA-binding transcriptional activator (NCBI) compare
b0830 yliB +0.3 2.4 predicted peptide transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b1066 rimJ +0.3 1.6 ribosomal-protein-S5-alanine N-acetyltransferase (NCBI) compare
b2555 yfhG +0.3 2.0 hypothetical protein (NCBI) compare
b0706 ybfD +0.3 0.9 hypothetical protein (NCBI) compare
b0852 rimK +0.3 1.7 ribosomal protein S6 modification protein (NCBI) compare
b0158 btuF +0.3 1.4 vitamin B12-transporter protein BtuF (NCBI) compare
b1141 ymfG +0.3 1.7 e14 prophage; predicted excisionase (NCBI) compare
b0617 citD +0.3 1.0 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b1632 rsxE +0.3 2.3 NADH-ubiquinone oxidoreductase (NCBI) compare
b2906 visC +0.3 1.9 hypothetical protein (NCBI) compare
b2510 b2510 +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b0121 speE +0.3 1.9 spermidine synthase (NCBI) compare
b3210 arcB +0.3 2.0 hybrid sensory histidine kinase in two-component regulatory system with ArcA (NCBI) compare
b0677 nagA +0.3 1.6 N-acetylglucosamine-6-phosphate deacetylase (NCBI) compare
b1197 treA +0.3 2.1 periplasmic trehalase (NCBI) compare
b1729 ydjN +0.3 1.9 predicted transporter (NCBI) compare
b2543 yphA +0.3 1.5 predicted inner membrane protein (RefSeq) compare
b0903 pflB +0.3 2.7 pyruvate formate lyase I (NCBI) compare
b1606 folM +0.3 1.6 short chain dehydrogenase (NCBI) compare
b1188 ycgB +0.3 1.8 ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) compare
b1587 ynfE +0.3 1.9 oxidoreductase subunit (NCBI) compare
b2572 rseA +0.3 1.8 anti-sigma factor (NCBI) compare
b3871 bipA +0.3 2.5 GTP-binding protein (NCBI) compare
b0542 renD +0.3 0.8 orf, hypothetical protein (VIMSS) compare
b2570 rseC +0.3 1.5 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b0196 rcsF +0.3 1.8 predicted outer membrane protein, signal (NCBI) compare
b1681 sufD +0.3 2.0 component of SufBCD complex (NCBI) compare
b1667 ydhR +0.3 0.7 hypothetical protein (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Sodium Chloride across organisms