Experiment set31IT028 for Escherichia coli BW25113

Compare to:

RCH2_defined_1percent_glucose with Sodium Chloride 200 mM

200 most detrimental genes:

  gene name fitness t score description  
b3261 fis +2.0 7.0 DNA-binding protein Fis (NCBI) compare
b3643 rph +1.4 3.6 ribonuclease PH (NCBI) compare
b1575 dicB +1.2 2.3 Qin prophage; cell division inhibition protein (NCBI) compare
b2642 yfjW +1.1 2.1 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b1278 pgpB +1.1 3.3 phosphatidylglycerophosphatase B (NCBI) compare
b2912 ygfA +1.0 3.5 putative ligase (VIMSS) compare
b0746 valZ +1.0 3.3 tRNA-Val (NCBI) compare
b4048 yjbM +0.9 2.1 hypothetical protein (NCBI) compare
b0059 hepA +0.9 8.2 ATP-dependent helicase HepA (NCBI) compare
b3863 polA +0.9 1.1 DNA polymerase I (NCBI) compare
b3977 tyrU +0.9 1.4 tRNA-Tyr (NCBI) compare
b2071 yegJ +0.9 2.5 hypothetical protein (NCBI) compare
b2297 pta +0.9 1.4 phosphate acetyltransferase (NCBI) compare
b1085 b1085 +0.9 1.8 orf, hypothetical protein (VIMSS) compare
b3761 trpT +0.9 2.2 tRNA-Trp (NCBI) compare
b1567 intK +0.8 2.8 Qin prophage; predicted protein (RefSeq) compare
b3387 dam +0.8 6.7 DNA adenine methylase (NCBI) compare
b4603 rseX +0.8 0.9 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b3341 rpsG +0.8 1.8 30S ribosomal protein S7 (NCBI) compare
b1493 gadB +0.7 1.1 glutamate decarboxylase B, PLP-dependent (NCBI) compare
b4000 hupA +0.7 4.6 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b3027 ygiZ +0.7 0.6 conserved inner membrane protein (NCBI) compare
b4522 yciX_1 +0.7 2.1 hypothetical protein (NCBI) compare
b3500 gor +0.7 1.4 glutathione reductase (NCBI) compare
b1412 azoR +0.6 3.0 acyl carrier protein phosphodiesterase (NCBI) compare
b2808 gcvA +0.6 3.4 DNA-binding transcriptional dual regulator (NCBI) compare
b2136 yohD +0.6 2.0 orf, hypothetical protein (VIMSS) compare
b0389 yaiA +0.6 2.2 hypothetical protein (NCBI) compare
b1302 puuE +0.6 2.5 GABA aminotransferase, PLP-dependent (NCBI) compare
b3790 rffC +0.6 2.7 TDP-fucosamine acetyltransferase (RefSeq) compare
b2597 yfiA +0.6 1.8 cold shock protein associated with 30S ribosomal subunit (NCBI) compare
b0131 panD +0.6 2.5 aspartate 1-decarboxylase precursor (NCBI) compare
b2904 gcvH +0.6 2.6 glycine cleavage system protein H (NCBI) compare
b1524 yneH +0.6 0.9 predicted glutaminase (NCBI) compare
b2259 pmrD +0.6 1.2 polymyxin resistance protein B (VIMSS) compare
b3181 greA +0.6 2.9 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1259 yciG +0.6 1.4 orf, hypothetical protein (VIMSS) compare
b2198 ccmD +0.6 1.9 cytochrome c biogenesis protein (NCBI) compare
b1332 ynaJ +0.5 0.5 predicted inner membrane protein (NCBI) compare
b2684 mprA +0.5 1.9 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b0661 miaB +0.5 3.9 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b1977 asnT +0.5 1.7 tRNA-Asn (NCBI) compare
b1642 slyA +0.5 1.4 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b2939 yqgB +0.5 1.5 predicted protein (RefSeq) compare
b4702 mgtL +0.5 1.5 regulatory leader peptide for mgtA (NCBI) compare
b4451 ryhB +0.5 1.1 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b4699 fnrS +0.5 0.7 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b3644 yicC +0.5 2.2 hypothetical protein (NCBI) compare
b3180 yhbY +0.5 3.6 predicted RNA-binding protein (NCBI) compare
b1961 dcm +0.5 1.1 DNA cytosine methylase (NCBI) compare
b0542 renD +0.5 1.1 orf, hypothetical protein (VIMSS) compare
b1724 ydiZ +0.5 1.2 hypothetical protein (NCBI) compare
b1298 ycjL +0.5 2.5 probable amidotransferase subunit (VIMSS) compare
b1138 ymfE +0.5 1.1 e14 prophage; predicted inner membrane protein (NCBI) compare
b1429 tehA +0.5 0.9 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b1355 b1355 +0.5 2.4 orf, hypothetical protein (VIMSS) compare
b0101 yacG +0.5 2.2 zinc-binding protein (NCBI) compare
b2300 yfcE +0.5 1.9 orf, hypothetical protein (VIMSS) compare
b2910 zapA +0.5 2.6 protein that localizes to the cytokinetic ring (NCBI) compare
b1593 b1593 +0.5 1.9 orf, hypothetical protein (VIMSS) compare
b4692 oweE +0.5 2.0 no description compare
b1305 pspB +0.5 1.2 phage shock protein B (NCBI) compare
b4592 yccB +0.5 1.4 hypothetical protein (RefSeq) compare
b1336 ydaH +0.5 2.4 putative pump protein (transport) (VIMSS) compare
b4407 thiS +0.5 1.2 sulfur carrier protein ThiS (NCBI) compare
b2903 gcvP +0.5 4.2 glycine dehydrogenase (NCBI) compare
b4370 leuQ +0.5 1.4 tRNA-Leu (NCBI) compare
b1790 yeaM +0.5 1.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b2156 lysP +0.5 3.8 lysine transporter (NCBI) compare
b0251 yafY +0.5 2.6 CP4-6 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b2905 gcvT +0.5 3.1 glycine cleavage system aminomethyltransferase T (NCBI) compare
b1300 puuC +0.5 2.8 gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent (NCBI) compare
b3260 dusB +0.5 1.4 tRNA-dihydrouridine synthase B (NCBI) compare
b2248 yfaX +0.5 1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b1088 yceD +0.5 1.7 hypothetical protein (NCBI) compare
b3545 proK +0.4 1.0 tRNA-Pro (NCBI) compare
b4458 oxyS +0.4 0.7 global regulatory RNA (NCBI) compare
b1160 elbA +0.4 0.7 hypothetical protein (NCBI) compare
b1920 fliY +0.4 1.7 cystine transporter subunit (NCBI) compare
b1222 narX +0.4 2.0 sensory histidine kinase in two-component regulatory system with NarL (NCBI) compare
b1486 ddpB +0.4 1.8 D-ala-D-ala transporter subunit (NCBI) compare
b4695 ykgT +0.4 1.5 no description compare
b4685 yrbN +0.4 1.4 expressed protein (RefSeq) compare
b1847 yebF +0.4 0.7 predicted protein (RefSeq) compare
b0227 yafL +0.4 2.2 predicted lipoprotein and C40 family peptidase (NCBI) compare
b1334 fnr +0.4 1.5 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b1827 kdgR +0.4 1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b1266 yciV +0.4 1.6 hypothetical protein (NCBI) compare
b1151 ymfO +0.4 1.6 e14 prophage; conserved protein (RefSeq) compare
b2688 gshA +0.4 2.9 glutamate--cysteine ligase (NCBI) compare
b1793 yoaF +0.4 1.8 conserved outer membrane protein (NCBI) compare
b3164 pnp +0.4 1.6 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b4589 ylcI +0.4 1.5 hypothetical protein (RefSeq) compare
b0933 ssuB +0.4 2.2 alkanesulfonate transporter subunit (NCBI) compare
b2083 yegZ +0.4 1.6 orf, hypothetical protein (VIMSS) compare
b1008 ycdI +0.4 2.5 hypothetical protein (NCBI) compare
b4373 rimI +0.4 1.7 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b1123 potD +0.4 1.6 spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI) compare
b0051 ksgA +0.4 2.0 dimethyladenosine transferase (NCBI) compare
b3149 yraO +0.4 2.8 DnaA initiator-associating factor for replication initiation (NCBI) compare
b1632 rsxE +0.4 2.8 NADH-ubiquinone oxidoreductase (NCBI) compare
b2080 b2080 +0.4 1.2 orf, hypothetical protein (VIMSS) compare
b4203 rplI +0.4 2.1 50S ribosomal protein L9 (NCBI) compare
b1291 sapD +0.4 1.0 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0798 ybiA +0.4 0.7 hypothetical protein (NCBI) compare
b2990 hybG +0.4 0.7 hydrogenase 2 accessory protein (NCBI) compare
b2951 yggS +0.4 2.0 predicted enzyme (NCBI) compare
b3670 ilvN +0.4 2.7 acetolactate synthase small subunit (NCBI) compare
b1861 ruvA +0.4 0.7 Holliday junction DNA helicase motor protein (NCBI) compare
b2309 hisJ +0.4 2.0 histidine/lysine/arginine/ornithine transporter subunit (NCBI) compare
b1758 b1758 +0.4 0.7 putative cytochrome oxidase (VIMSS) compare
b0622 crcA +0.4 2.7 palmitoyl transferase for Lipid A (NCBI) compare
b1777 b1777 +0.4 1.6 orf, hypothetical protein (VIMSS) compare
b4504 ykfH +0.4 1.9 hypothetical protein (NCBI) compare
b4397 creA +0.4 2.2 hypothetical protein (NCBI) compare
b2125 yehT +0.4 1.9 orf, hypothetical protein (VIMSS) compare
b4402 yjjY +0.4 0.6 hypothetical protein (NCBI) compare
b4594 ymgJ +0.4 1.2 hypothetical protein (RefSeq) compare
b4567 yjjZ +0.4 2.4 hypothetical protein (NCBI) compare
b2704 srlB +0.4 1.2 glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI) compare
b4345 mcrC +0.4 0.7 5-methylcytosine-specific restriction enzyme McrBC, subunit McrC (NCBI) compare
b1377 ompN +0.4 2.0 outer membrane pore protein N, non-specific (NCBI) compare
b1728 ydjM +0.4 1.6 predicted inner membrane protein regulated by LexA (RefSeq) compare
b1558 cspF +0.4 1.1 Qin prophage; cold shock protein (NCBI) compare
b1518 lsrG +0.4 1.0 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b2123 yehR +0.4 1.5 orf, hypothetical protein (VIMSS) compare
b0685 ybfE +0.4 1.4 orf, hypothetical protein (VIMSS) compare
b3786 rffE +0.4 3.1 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b1195 ymgE +0.4 1.2 predicted inner membrane protein (NCBI) compare
b4650 yibS +0.4 1.2 no description compare
b0195 yaeB +0.4 2.0 hypothetical protein (NCBI) compare
b1804 rnd +0.4 1.8 ribonuclease D (NCBI) compare
b3107 yhaL +0.4 0.9 orf, hypothetical protein (VIMSS) compare
b1763 topB +0.4 1.4 DNA topoisomerase III (NCBI) compare
b3153 yhbO +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b4171 miaA +0.4 1.5 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b4570 lomR +0.4 1.1 no description compare
b0440 hupB +0.4 2.1 HU, DNA-binding transcriptional regulator, beta subunit (NCBI) compare
b2572 rseA +0.3 2.4 anti-sigma factor (NCBI) compare
b2058 wcaB +0.3 0.6 predicted acyl transferase (NCBI) compare
b1598 ydgD +0.3 1.9 predicted peptidase (NCBI) compare
b1931 yedK +0.3 2.0 hypothetical protein (NCBI) compare
b1144 ymfJ +0.3 1.2 orf, hypothetical protein (VIMSS) compare
b4461 yfjD +0.3 2.4 predicted inner membrane protein (RefSeq) compare
b1296 ycjJ +0.3 1.8 putative amino acid/amine transport protein (VIMSS) compare
b2953 yggU +0.3 0.5 orf, hypothetical protein (VIMSS) compare
b1109 ndh +0.3 1.5 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b2457 cchA +0.3 2.1 detox protein (VIMSS) compare
b4391 yjjK +0.3 2.6 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1190 dadX +0.3 1.8 alanine racemase (NCBI) compare
b2532 yfhQ +0.3 2.1 predicted methyltransferase (NCBI) compare
b2435 amiA +0.3 1.3 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b1680 sufS +0.3 2.4 selenocysteine lyase (NCBI) compare
b1539 ydfG +0.3 1.6 L-allo-threonine dehydrogenase, NAD(P)-binding (NCBI) compare
b4548 ypjJ +0.3 2.1 hypothetical protein (NCBI) compare
b0912 ihfB +0.3 2.0 integration host factor subunit beta (NCBI) compare
b2575 yfiC +0.3 2.6 putative enzyme (VIMSS) compare
b1310 ycjN +0.3 1.7 predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b1119 nagK +0.3 1.9 N-acetyl-D-glucosamine kinase (NCBI) compare
b0975 hyaD +0.3 1.5 protein involved in processing of HyaA and HyaB proteins (NCBI) compare
b0976 hyaE +0.3 2.0 protein involved in processing of HyaA and HyaB proteins (NCBI) compare
b2434 b2434 +0.3 1.6 orf, hypothetical protein (VIMSS) compare
b2634 yfjR +0.3 1.8 CP4-57 prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b3677 yidI +0.3 2.0 predicted inner membrane protein (NCBI) compare
b1888 cheA +0.3 1.8 fused chemotactic sensory histidine kinase in two-component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein (NCBI) compare
b1339 abgR +0.3 2.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b2934 cmtB +0.3 0.4 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1583 ynfB +0.3 0.8 hypothetical protein (NCBI) compare
b2911 ssrS +0.3 0.9 6S regulatory RNA (NCBI) compare
b2749 ygbE +0.3 2.1 conserved inner membrane protein (NCBI) compare
b0197 metQ +0.3 2.4 DL-methionine transporter subunit (NCBI) compare
b0326 yahL +0.3 0.9 hypothetical protein (NCBI) compare
b0479 fsr +0.3 2.2 predicted fosmidomycin efflux system (NCBI) compare
b1013 ycdC +0.3 1.1 predicted DNA-binding transcriptional regulator (NCBI) compare
b4521 ycgI +0.3 1.2 hypothetical protein (NCBI) compare
b4503 yafF +0.3 0.3 no description compare
b1197 treA +0.3 2.3 periplasmic trehalase (NCBI) compare
b1282 yciH +0.3 1.7 orf, hypothetical protein (VIMSS) compare
b4552 yrhC +0.3 0.4 no description compare
b0706 ybfD +0.3 0.9 hypothetical protein (NCBI) compare
b1120 cobB +0.3 1.4 deacetylase of acs and cheY, regulates chemotaxis (NCBI) compare
b2159 nfo +0.3 2.1 endonuclease IV (NCBI) compare
b1926 fliT +0.3 1.4 predicted chaperone (NCBI) compare
b4519 icdC +0.3 1.4 no description compare
b3740 gidB +0.3 2.5 glucose-inhibited division protein B (NCBI) compare
b2490 b2490 +0.3 1.2 putative protein processing element (VIMSS) compare
b4030 yjbA +0.3 1.7 phosphate-starvation-inducible protein PsiE (NCBI) compare
b0813 rhtA +0.3 2.2 threonine and homoserine efflux system (NCBI) compare
b4640 yoeG +0.3 0.9 no description compare
b4481 rffT +0.3 1.7 4-alpha-L-fucosyltransferase (NCBI) compare
b3392 yrfA +0.3 2.2 orf, hypothetical protein (VIMSS) compare
b3337 bfd +0.3 1.7 bacterioferritin-associated ferredoxin (NCBI) compare
b4686 yshB +0.3 1.8 expressed protein (RefSeq) compare
b0401 brnQ +0.3 1.9 predicted branched chain amino acid transporter (LIV-II) (NCBI) compare
b0605 ahpC +0.3 1.3 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b2169 fruB +0.3 1.5 fused fructose-specific PTS enzymes: IIA component/HPr component (NCBI) compare
b4435 isrC +0.3 0.9 unknown RNA (NCBI) compare
b2643 yfjX +0.3 1.8 CP4-57 prophage; predicted antirestriction protein (NCBI) compare
b4363 yjjB +0.3 1.3 conserved inner membrane protein (RefSeq) compare
b3443 yrhA +0.3 0.5 orf, hypothetical protein (VIMSS) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Sodium Chloride across organisms