Experiment set31IT027 for Escherichia coli BW25113

Compare to:

RCH2_defined_1percent_glucose with Sodium Chloride 100 mM

200 most detrimental genes:

  gene name fitness t score description  
b3261 fis +2.5 10.2 DNA-binding protein Fis (NCBI) compare
b3979 thrT +1.9 1.6 tRNA-Thr (NCBI) compare
b3643 rph +1.7 4.2 ribonuclease PH (NCBI) compare
b0059 hepA +1.3 11.8 ATP-dependent helicase HepA (NCBI) compare
b1936 intG +1.2 2.1 predicted defective phage integrase (pseudogene) (RefSeq) compare
b2904 gcvH +1.2 4.8 glycine cleavage system protein H (NCBI) compare
b3341 rpsG +1.2 2.6 30S ribosomal protein S7 (NCBI) compare
b3977 tyrU +1.2 2.0 tRNA-Tyr (NCBI) compare
b2808 gcvA +1.2 6.3 DNA-binding transcriptional dual regulator (NCBI) compare
b2903 gcvP +1.0 8.9 glycine dehydrogenase (NCBI) compare
b1724 ydiZ +1.0 2.1 hypothetical protein (NCBI) compare
b1164 ycgZ +1.0 2.9 hypothetical protein (NCBI) compare
b2071 yegJ +1.0 2.2 hypothetical protein (NCBI) compare
b2058 wcaB +0.9 2.0 predicted acyl transferase (NCBI) compare
b2189 proL +0.9 2.8 tRNA-Pro (NCBI) compare
b0746 valZ +0.9 3.1 tRNA-Val (NCBI) compare
b3260 dusB +0.9 2.4 tRNA-dihydrouridine synthase B (NCBI) compare
b1038 csgF +0.8 3.0 predicted transport protein (NCBI) compare
b2905 gcvT +0.8 6.1 glycine cleavage system aminomethyltransferase T (NCBI) compare
b1462 b1462 +0.8 3.7 orf, hypothetical protein (VIMSS) compare
b0232 yafN +0.8 0.5 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b4000 hupA +0.8 5.1 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b0881 clpS +0.8 2.2 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b4460 araH +0.8 1.4 fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq) compare
b3387 dam +0.8 6.9 DNA adenine methylase (NCBI) compare
b1942 fliJ +0.8 2.2 flagellar biosynthesis chaperone (NCBI) compare
b1278 pgpB +0.8 3.4 phosphatidylglycerophosphatase B (NCBI) compare
b3181 greA +0.8 3.1 transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS) compare
b1728 ydjM +0.7 2.5 predicted inner membrane protein regulated by LexA (RefSeq) compare
b1793 yoaF +0.7 3.1 conserved outer membrane protein (NCBI) compare
b2934 cmtB +0.7 0.9 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1434 ydcN +0.7 2.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b0014 dnaK +0.7 2.5 molecular chaperone DnaK (NCBI) compare
b2688 gshA +0.7 5.3 glutamate--cysteine ligase (NCBI) compare
b1334 fnr +0.7 2.4 DNA-binding transcriptional dual regulator, global regulator of anaerobic growth (NCBI) compare
b2504 yfgG +0.7 1.6 Negative regulator of nickel and cobalt uptake (YfgG) (from data) compare
b1429 tehA +0.7 0.9 potassium-tellurite ethidium and proflavin transporter (NCBI) compare
b1190 dadX +0.7 3.7 alanine racemase (NCBI) compare
b1357 ydaS +0.7 1.8 Rac prophage; predicted DNA-binding transcriptional regulator (NCBI) compare
b0865 ybjP +0.7 2.7 predicted lipoprotein (NCBI) compare
b2951 yggS +0.7 4.4 predicted enzyme (NCBI) compare
b2684 mprA +0.7 2.3 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b0998 torD +0.7 1.4 chaperone protein TorD (NCBI) compare
b0051 ksgA +0.7 3.4 dimethyladenosine transferase (NCBI) compare
b3844 fre +0.7 3.5 NAD(P)H-flavin reductase (NCBI) compare
b2125 yehT +0.7 3.1 orf, hypothetical protein (VIMSS) compare
b2829 ptsP +0.6 5.3 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b2300 yfcE +0.6 2.9 orf, hypothetical protein (VIMSS) compare
b1847 yebF +0.6 1.6 predicted protein (RefSeq) compare
b1542 ydfI +0.6 2.4 predicted mannonate dehydrogenase (NCBI) compare
b2659 b2659 +0.6 1.9 orf, hypothetical protein (VIMSS) compare
b2990 hybG +0.6 1.4 hydrogenase 2 accessory protein (NCBI) compare
b0018 mokC +0.6 1.2 regulatory protein for HokC, overlaps CDS of hokC (NCBI) compare
b4533 ynfO +0.6 1.9 Qin prophage; predicted protein (NCBI) compare
b1642 slyA +0.6 1.4 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b4201 priB +0.6 1.9 primosomal replication protein N (NCBI) compare
b4458 oxyS +0.6 1.1 global regulatory RNA (NCBI) compare
b4538 yoeF +0.6 1.3 hypothetical protein (RefSeq) compare
b1726 yniB +0.6 2.0 predicted inner membrane protein (NCBI) compare
b3052 rfaE +0.6 3.0 fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (NCBI) compare
b2080 b2080 +0.6 1.8 orf, hypothetical protein (VIMSS) compare
b1849 purT +0.6 3.3 phosphoribosylglycinamide formyltransferase 2 (NCBI) compare
b1841 yobA +0.6 1.3 hypothetical protein (NCBI) compare
b4532 yncN +0.6 3.1 orf, hypothetical protein (VIMSS) compare
b1085 b1085 +0.6 1.2 orf, hypothetical protein (VIMSS) compare
b1259 yciG +0.6 1.3 orf, hypothetical protein (VIMSS) compare
b1824 yobF +0.5 2.2 hypothetical protein (NCBI) compare
b0661 miaB +0.5 4.6 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) compare
b0296 ykgM +0.5 1.7 50S ribosomal protein L31 (NCBI) compare
b0440 hupB +0.5 2.4 HU, DNA-binding transcriptional regulator, beta subunit (NCBI) compare
b2432 yfeY +0.5 2.2 hypothetical protein (NCBI) compare
b1454 yncG +0.5 2.1 predicted enzyme (NCBI) compare
b1299 puuR +0.5 3.2 DNA-binding transcriptional repressor (NCBI) compare
b3620 rfaF +0.5 3.1 ADP-heptose:LPS heptosyltransferase II (NCBI) compare
b1842 holE +0.5 0.9 DNA polymerase III, theta subunit (NCBI) compare
b1823 cspC +0.5 1.5 stress protein, member of the CspA-family (NCBI) compare
b4030 yjbA +0.5 2.2 phosphate-starvation-inducible protein PsiE (NCBI) compare
b3229 sspA +0.5 2.4 stringent starvation protein A (NCBI) compare
b1575 dicB +0.5 1.3 Qin prophage; cell division inhibition protein (NCBI) compare
b2597 yfiA +0.5 1.5 cold shock protein associated with 30S ribosomal subunit (NCBI) compare
b1977 asnT +0.5 1.3 tRNA-Asn (NCBI) compare
b1138 ymfE +0.5 1.1 e14 prophage; predicted inner membrane protein (NCBI) compare
b4171 miaA +0.5 2.0 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b4653 yicT +0.5 1.4 no description compare
b1109 ndh +0.5 2.8 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b4592 yccB +0.5 1.5 hypothetical protein (RefSeq) compare
b1961 dcm +0.5 1.1 DNA cytosine methylase (NCBI) compare
b2330 yfcB +0.5 0.8 putative adenine-specific methylase (VIMSS) compare
b1148 ymfM +0.5 1.9 e14 prophage; predicted protein (NCBI) compare
b3119 tdcR +0.5 1.5 threonine dehydratase operon activator protein (VIMSS) compare
b1222 narX +0.5 2.6 sensory histidine kinase in two-component regulatory system with NarL (NCBI) compare
b1629 rsxC +0.5 2.7 electron transport complex protein RnfC (NCBI) compare
b1569 dicC +0.5 2.0 Qin prophage; DNA-binding transcriptional regulator for DicB (NCBI) compare
b0196 rcsF +0.5 2.0 predicted outer membrane protein, signal (NCBI) compare
b1914 uvrY +0.5 2.4 response regulator (NCBI) compare
b1426 ydcH +0.5 1.2 orf, hypothetical protein (VIMSS) compare
b0630 lipB +0.5 1.2 protein of lipoate biosynthesis (VIMSS) compare
b3998 nfi +0.5 3.1 endonuclease V (deoxyinosine 3'endoduclease) (VIMSS) compare
b1651 gloA +0.5 2.2 glyoxalase I, Ni-dependent (NCBI) compare
b2050 wcaI +0.5 1.7 predicted glycosyl transferase (NCBI) compare
b2618 b2618 +0.5 1.8 orf, hypothetical protein (VIMSS) compare
b3484 yhhI +0.4 1.0 predicted transposase (NCBI) compare
b1013 ycdC +0.4 1.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b1090 plsX +0.4 2.0 glycerolphosphate auxotrophy in plsB background (VIMSS) compare
b4450 arcZ +0.4 1.8 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b1650 nemA +0.4 2.2 N-ethylmaleimide reductase, FMN-linked (NCBI) compare
b2786 barA +0.4 4.1 hybrid sensory histidine kinase, in two-component regulatory system with UvrY (NCBI) compare
b3621 rfaC +0.4 2.6 ADP-heptose:LPS heptosyl transferase I (NCBI) compare
b3545 proK +0.4 0.9 tRNA-Pro (NCBI) compare
b3206 npr +0.4 2.6 phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI) compare
b1355 b1355 +0.4 2.0 orf, hypothetical protein (VIMSS) compare
b4203 rplI +0.4 1.9 50S ribosomal protein L9 (NCBI) compare
b3199 yrbK +0.4 1.2 hypothetical protein (NCBI) compare
b1233 ychJ +0.4 1.9 hypothetical protein (NCBI) compare
b4363 yjjB +0.4 1.9 conserved inner membrane protein (RefSeq) compare
b2532 yfhQ +0.4 1.9 predicted methyltransferase (NCBI) compare
b1418 cybB +0.4 1.5 cytochrome b(561) (VIMSS) compare
b0222 lpcA +0.4 1.5 phosphoheptose isomerase (NCBI) compare
b2572 rseA +0.4 2.6 anti-sigma factor (NCBI) compare
b3644 yicC +0.4 2.0 hypothetical protein (NCBI) compare
b2912 ygfA +0.4 1.8 putative ligase (VIMSS) compare
b2301 yfcF +0.4 2.2 predicted enzyme (NCBI) compare
b1627 rsxA +0.4 2.0 Na(+)-translocating NADH-quinone reductase subunit E (NCBI) compare
b1253 yciA +0.4 1.3 predicted hydrolase (NCBI) compare
b4373 rimI +0.4 1.0 ribosomal-protein-alanine N-acetyltransferase (NCBI) compare
b3631 rfaG +0.4 3.6 glucosyltransferase I (NCBI) compare
b0504 ybbS +0.4 1.5 DNA-binding transcriptional activator of the allD operon (NCBI) compare
b1241 adhE +0.4 3.0 fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase (NCBI) compare
b1758 b1758 +0.4 0.7 putative cytochrome oxidase (VIMSS) compare
b2567 rnc +0.4 0.4 ribonuclease III (NCBI) compare
b1689 b1689 +0.4 1.7 orf, hypothetical protein (VIMSS) compare
b1988 nac +0.4 1.8 DNA-binding transcriptional dual regulator of nitrogen assimilation (NCBI) compare
b2435 amiA +0.4 1.7 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b1461 ydcE +0.4 1.1 4-oxalocrotonate tautomerase (NCBI) compare
b3706 trmE +0.4 1.5 tRNA modification GTPase (NCBI) compare
b1300 puuC +0.4 1.7 gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent (NCBI) compare
b2539 hcaF +0.4 1.9 3-phenylpropionate dioxygenase, small (beta) subunit (NCBI) compare
b1630 rsxD +0.4 2.8 electron transport complex protein RnfD (NCBI) compare
b0195 yaeB +0.4 2.8 hypothetical protein (NCBI) compare
b1539 ydfG +0.4 1.4 L-allo-threonine dehydrogenase, NAD(P)-binding (NCBI) compare
b0949 uup +0.4 2.1 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b3391 hofQ +0.4 3.1 predicted fimbrial transporter (NCBI) compare
b1021 ycdP +0.4 1.9 predicted inner membrane protein (NCBI) compare
b4371 rsmC +0.4 2.6 16S ribosomal RNA m2G1207 methyltransferase (NCBI) compare
b1930 yedF +0.4 2.3 hypothetical protein (NCBI) compare
b1514 lsrC +0.4 0.7 AI2 transporter (NCBI) compare
b4589 ylcI +0.4 1.3 hypothetical protein (RefSeq) compare
b3858 yihD +0.4 1.7 hypothetical protein (NCBI) compare
b4436 ryeC +0.4 0.6 Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq) compare
b1960 vsr +0.4 1.8 DNA mismatch endonuclease of very short patch repair (NCBI) compare
b2237 inaA +0.4 1.9 hypothetical protein (NCBI) compare
b3929 rraA +0.4 2.3 ribonuclease activity regulator protein RraA (NCBI) compare
b4011 yjaA +0.4 1.2 hypothetical protein (NCBI) compare
b4227 ytfQ +0.4 2.1 predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b3871 bipA +0.4 2.9 GTP-binding protein (NCBI) compare
b1916 sdiA +0.4 2.7 DNA-binding transcriptional activator (NCBI) compare
b1827 kdgR +0.4 1.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b1518 lsrG +0.4 1.0 autoinducer-2 (AI-2) modifying protein LsrG (NCBI) compare
b1984 asnW +0.3 0.7 tRNA-Asn (NCBI) compare
b2741 rpoS +0.3 2.1 RNA polymerase sigma factor (NCBI) compare
b3624 rfaZ +0.3 1.1 lipopolysaccharide core biosynthesis protein (NCBI) compare
b2357 yfdN +0.3 1.3 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b0999 cbpM +0.3 1.6 modulator of CbpA co-chaperone (NCBI) compare
b4609 ryfD +0.3 1.0 Novel sRNA, function unknown (RefSeq) compare
b1922 fliA +0.3 2.0 flagellar biosynthesis; alternative sigma factor 28; regulation of flagellar operons (VIMSS) compare
b4622 ytcA +0.3 1.8 hypothetical protein (RefSeq) compare
b2040 rfbD +0.3 2.7 dTDP-4-dehydrorhamnose reductase subunit, NAD(P)-binding, of dTDP-L-rhamnose synthase (NCBI) compare
b4066 yjcF +0.3 0.4 hypothetical protein (NCBI) compare
b3786 rffE +0.3 2.9 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b1419 ydcA +0.3 2.0 hypothetical protein (NCBI) compare
b2569 lepA +0.3 1.6 GTP-binding protein LepA (NCBI) compare
b4695 ykgT +0.3 1.2 no description compare
b1431 ydcL +0.3 2.1 predicted lipoprotein (NCBI) compare
b1704 aroH +0.3 1.8 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, tryptophan repressible (NCBI) compare
b4606 ypfM +0.3 1.9 hypothetical protein (RefSeq) compare
b4519 icdC +0.3 1.5 no description compare
b4418 sraB +0.3 1.1 unknown RNA (NCBI) compare
b1795 yeaQ +0.3 1.5 conserved inner membrane protein (NCBI) compare
b1465 narV +0.3 0.7 nitrate reductase 2 (NRZ), gamma subunit (NCBI) compare
b4655 ythA +0.3 0.7 expressed protein (RefSeq) compare
b0236 prfH +0.3 1.4 peptide chain release factor 2 (NCBI) compare
b2266 elaB +0.3 1.2 hypothetical protein (NCBI) compare
b1632 rsxE +0.3 2.6 NADH-ubiquinone oxidoreductase (NCBI) compare
b4215 ytfI +0.3 0.9 hypothetical protein (NCBI) compare
b0365 tauA +0.3 2.0 taurine transport system periplasmic protein (VIMSS) compare
b1523 yneG +0.3 0.9 hypothetical protein (NCBI) compare
b3842 rfaH +0.3 2.2 transcriptional activator RfaH (NCBI) compare
b1633 nth +0.3 1.8 DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) (NCBI) compare
b1141 ymfG +0.3 1.9 e14 prophage; predicted excisionase (NCBI) compare
b2156 lysP +0.3 2.1 lysine transporter (NCBI) compare
b2947 gshB +0.3 1.8 glutathione synthetase (NCBI) compare
b0759 galE +0.3 2.1 UDP-galactose-4-epimerase (VIMSS) compare
b3085 ygjP +0.3 1.5 orf, hypothetical protein (VIMSS) compare
b1725 yniA +0.3 1.6 predicted phosphotransferase/kinase (NCBI) compare
b1804 rnd +0.3 1.8 ribonuclease D (NCBI) compare
b3630 rfaP +0.3 2.7 kinase that phosphorylates core heptose of lipopolysaccharide (NCBI) compare
b1868 yecE +0.3 1.0 hypothetical protein (NCBI) compare
b1088 yceD +0.3 1.2 hypothetical protein (NCBI) compare
b2937 speB +0.3 2.1 agmatinase (NCBI) compare
b4435 isrC +0.3 1.0 unknown RNA (NCBI) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in stress experiments

For stress Sodium Chloride across organisms