Experiment set30IT059 for Pseudomonas putida KT2440

Acanthamoeba sp., muxed culture-1

Group: micoeukaryotes
Media: LB (0.1x) + Pelleted after growth overnight in LB+Kan50 + CaCl2, MgSO4 (10mM ), pH=7
Culturing: Putida_ML5, 48-well_plate, Aerobic, at 30 (C), shaken=700 rpm
By: VM on 6/23/21
Media components: 1 g/L Tryptone, 0.5 g/L Yeast Extract, 0.5 g/L Sodium Chloride (final concentrations)

Specific Phenotypes

For 7 genes in this experiment

For micoeukaryotes Pelleted after growth overnight in LB+Kan50 in Pseudomonas putida KT2440

For micoeukaryotes Pelleted after growth overnight in LB+Kan50 across organisms

SEED Subsystems

Subsystem	#Specific
Ammonia assimilation	1
Flagellum	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
High affinity phosphate transporter and control of PHO regulon	1
Lysine degradation	1
Phosphate metabolism	1
Polyamine Metabolism	1
Proteasome bacterial	1
Proteolysis in bacteria, ATP-dependent	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glutamate metabolism
• Lysine degradation
• Tryptophan metabolism
• Nitrogen metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamate biosynthesis I	2	2	2
L-glutamine degradation I	1	1	1
L-glutamine degradation II	1	1	1
ammonia assimilation cycle III	3	3	2
indole-3-acetate biosynthesis III (bacteria)	2	2	1
L-glutamate and L-glutamine biosynthesis	7	6	2
L-asparagine biosynthesis III (tRNA-dependent)	4	4	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	3	1
L-lysine degradation IV	5	5	1
L-citrulline biosynthesis	8	7	1
superpathway of L-citrulline metabolism	12	9	1
superpathway of L-lysine degradation	43	23	1