Experiment set30IT053 for Pseudomonas putida KT2440

Tetrahymena sp., Mixed culture-1

Group: micoeukaryotes
Media: LB (0.1x) + Pelleted after growth overnight in LB+Kan50 + CaCl2, MgSO4 (10mM ), pH=7
Culturing: Putida_ML5, 48-well_plate, Aerobic, at 30 (C), shaken=700 rpm
By: VM on 6/23/21
Media components: 1 g/L Tryptone, 0.5 g/L Yeast Extract, 0.5 g/L Sodium Chloride (final concentrations)

Specific Phenotypes

For 12 genes in this experiment

For micoeukaryotes Pelleted after growth overnight in LB+Kan50 in Pseudomonas putida KT2440

For micoeukaryotes Pelleted after growth overnight in LB+Kan50 across organisms

SEED Subsystems

Subsystem	#Specific
Polyamine Metabolism	2
Biogenesis of cytochrome c oxidases	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Tyrosine metabolism
• Drug metabolism - cytochrome P450
• Urea cycle and metabolism of amino groups
• Glycine, serine and threonine metabolism
• Arginine and proline metabolism
• Histidine metabolism
• Phenylalanine metabolism
• Tryptophan metabolism
• Glutathione metabolism
• Styrene degradation
• Alkaloid biosynthesis I
• Metabolism of xenobiotics by cytochrome P450

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
phenylethylamine degradation I	2	2	1
L-tryptophan degradation VI (via tryptamine)	3	2	1
gentisate degradation I	3	1	1
noradrenaline and adrenaline degradation	13	8	4
glycine betaine degradation III	7	7	2
L-tryptophan degradation X (mammalian, via tryptamine)	4	3	1
putrescine degradation III	4	3	1
salidroside biosynthesis	4	3	1
aromatic biogenic amine degradation (bacteria)	8	3	2
melatonin degradation II	4	1	1
4-hydroxy-2-nonenal detoxification	4	1	1
L-tyrosine degradation I	5	5	1
phenylethanol biosynthesis	5	3	1
pentachlorophenol degradation	10	4	2
dopamine degradation	5	2	1
5-nitroanthranilate degradation	6	2	1
serotonin degradation	7	4	1
glutathione-mediated detoxification I	8	3	1
L-phenylalanine degradation IV (mammalian, via side chain)	9	6	1
gliotoxin biosynthesis	9	2	1
glutathione-mediated detoxification II	9	1	1
3-phenylpropanoate degradation	10	4	1
superpathway of phenylethylamine degradation	11	11	1
superpathway of melatonin degradation	11	1	1
indole glucosinolate activation (intact plant cell)	12	3	1
camalexin biosynthesis	12	2	1