Experiment set2S98 for Escherichia coli ECRC101

D-Glucose

Group: carbon source
Media: M9_plus_D-Glucose + D-Glucose (20 mM)
Culturing: Ecoli_ECRC101_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 12/16/24

Specific Phenotypes

For 8 genes in this experiment

For carbon source D-Glucose in Escherichia coli ECRC101

For carbon source D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
Cysteine Biosynthesis	3
Coenzyme A Biosynthesis	2
Methionine Biosynthesis	2
Fermentations: Mixed acid	1
Pyruvate metabolism I: anaplerotic reactions, PEP	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pantothenate and CoA biosynthesis
• Purine metabolism
• Methionine metabolism
• beta-Alanine metabolism
• Selenoamino acid metabolism
• Pyruvate metabolism
• Carbon fixation in photosynthetic organisms
• Reductive carboxylate cycle (CO2 fixation)
• Sulfur metabolism
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
phosphopantothenate biosynthesis I	4	4	2
sulfate activation for sulfonation	2	2	1
CO2 fixation into oxaloacetate (anaplerotic)	2	2	1
assimilatory sulfate reduction III	3	3	1
molybdenum cofactor biosynthesis	3	3	1
S-adenosyl-L-methionine salvage II	3	2	1
assimilatory sulfate reduction II	3	1	1
sulfite oxidation III	3	1	1
S-adenosyl-L-methionine salvage I	4	4	1
assimilatory sulfate reduction I	4	4	1
assimilatory sulfate reduction IV	4	3	1
L-methionine biosynthesis III	4	2	1
phosphopantothenate biosynthesis III (archaea)	4	2	1
superpathway of coenzyme A biosynthesis I (bacteria)	9	9	2
L-methionine biosynthesis I	5	5	1
seleno-amino acid biosynthesis (plants)	5	3	1
superpathway of coenzyme A biosynthesis II (plants)	10	5	2
selenate reduction	5	2	1
dissimilatory sulfate reduction I (to hydrogen sufide))	5	1	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	10	2
L-methionine biosynthesis II	6	5	1
superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans)	6	1	1
superpathway of sulfur metabolism (Desulfocapsa sulfoexigens)	6	1	1
C4 photosynthetic carbon assimilation cycle, NADP-ME type	7	4	1
superpathway of L-homoserine and L-methionine biosynthesis	8	8	1
partial TCA cycle (obligate autotrophs)	8	7	1
nitrogen remobilization from senescing leaves	8	5	1
superpathway of S-adenosyl-L-methionine biosynthesis	9	9	1
superpathway of sulfate assimilation and cysteine biosynthesis	9	9	1
superpathway of L-methionine biosynthesis (transsulfuration)	9	9	1
superpathway of seleno-compound metabolism	19	6	2
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)	10	6	1
C4 photosynthetic carbon assimilation cycle, NAD-ME type	11	8	1
superpathway of sulfide oxidation (phototrophic sulfur bacteria)	12	2	1
formaldehyde assimilation I (serine pathway)	13	8	1
C4 photosynthetic carbon assimilation cycle, PEPCK type	14	9	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	1
mixed acid fermentation	16	16	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	18	1
gluconeogenesis II (Methanobacterium thermoautotrophicum)	18	9	1
aspartate superpathway	25	24	1
ethene biosynthesis V (engineered)	25	18	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	22	1