Experiment set2S915 for Pseudomonas aeruginosa MRSN321

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carbon source 10 mM (+)-Sodium L-ascorbate

Group: carbon source
Media: Hans_Basal_Media + (+) Sodium L-ascorbate (10 mM), pH=7
Culturing: Paeruginosa_MRSN321_ML2, 96 deep-well block, Aerobic, at 30 (C), shaken=700 rpm
By: Hans and Ryan on 7/7/2025
Media components: 0.03 M PIPES sesquisodium salt, 0.1 g/L Potassium Chloride, 0.01 g/L Sodium Chloride, 0.01 g/L Calcium chloride dihydrate, 0.1 g/L Magnesium chloride hexahydrate, 0.1 g/L Sodium sulfate, 0.25 g/L Ammonium chloride, 0.1 g/L Disodium phosphate, DL vitamins (0.0002 mg/L biotin, 0.0002 mg/L Folic Acid, 0.001 mg/L Pyridoxine HCl, 0.0005 mg/L Riboflavin, 0.0005 mg/L Thiamine HCl, 0.0005 mg/L Nicotinic Acid, 0.0005 mg/L calcium pantothenate, 1e-05 mg/L Cyanocobalamin, 0.0005 mg/L 4-Aminobenzoic acid, 0.0005 mg/L Lipoic acid), Sulfur-free DL minerals (0.0003 g/L Magnesium chloride hexahydrate, 0.00015 g/L Nitrilotriacetic acid disodium salt, 0.0001 g/L Sodium Chloride, 5e-05 g/L Manganese (II) chloride tetrahydrate, 1e-05 g/L Cobalt chloride hexahydrate, 1.3e-05 g/L Zinc chloride, 1e-05 g/L Calcium chloride dihydrate, 1e-05 g/L Iron (II) chloride tetrahydrate, 2.5e-06 g/L Nickel (II) chloride hexahydrate, 2e-06 g/L Aluminum chloride hydrate, 1e-06 g/L copper (II) chloride dihydrate, 1e-06 g/L Boric Acid, 1e-06 g/L Sodium Molybdate Dihydrate, 3e-05 g/L Sodium selenite pentahydrate, 2.5e-05 g/L Sodium tungstate dihydrate)

Specific Phenotypes

For 36 genes in this experiment

For carbon source (+) Sodium L-ascorbate in Pseudomonas aeruginosa MRSN321

For carbon source (+) Sodium L-ascorbate across organisms

SEED Subsystems

Subsystem #Specific
Arginine and Ornithine Degradation 2
Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes 2
Arginine Biosynthesis extended 1
Biogenesis of c-type cytochromes 1
Branched-Chain Amino Acid Biosynthesis 1
Coenzyme B12 biosynthesis 1
D-galactarate, D-glucarate and D-glycerate catabolism 1
Glutathione-dependent pathway of formaldehyde detoxification 1
Glutathione: Biosynthesis and gamma-glutamyl cycle 1
Glutathione: Non-redox reactions 1
Glycine reductase, sarcosine reductase and betaine reductase 1
Glycolate, glyoxylate interconversions 1
Lactate utilization 1
Methylglyoxal Metabolism 1
Murein hydrolase regulation and cell death 1
Oxidative stress 1
Periplasmic disulfide interchange 1
Photorespiration (oxidative C2 cycle) 1
Potassium homeostasis 1
Ribosomal protein S12p Asp methylthiotransferase 1
Thioredoxin-disulfide reductase 1
Utilization of glutathione as a sulphur source 1
Wyeosine-MimG Biosynthesis 1
ZZ gjo need homes 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
acetaldehyde biosynthesis I 1 1 1
L-methionine degradation II 3 2 2
superoxide radicals degradation 2 2 1
ethanol degradation I 2 2 1
L-threonine degradation I 6 5 3
pyruvate fermentation to ethanol II 2 1 1
L-threonine degradation V 2 1 1
thioredoxin pathway 2 1 1
glutathione degradation (DUG pathway) 2 1 1
methanol oxidation to formaldehyde IV 2 1 1
L-isoleucine biosynthesis I (from threonine) 7 7 3
ethanol degradation II 3 3 1
formaldehyde oxidation II (glutathione-dependent) 3 3 1
L-lyxonate degradation 3 3 1
ethanol degradation IV 3 3 1
L-leucine degradation III 3 2 1
L-isoleucine degradation II 3 2 1
pyruvate fermentation to ethanol III 3 2 1
methylglyoxal degradation VIII 3 2 1
pyruvate fermentation to ethanol I 3 2 1
methylglyoxal degradation I 3 2 1
L-valine degradation II 3 2 1
L-methionine degradation III 3 1 1
hypoglycin biosynthesis 14 4 4
reactive oxygen species degradation 4 4 1
phytol degradation 4 3 1
L-phenylalanine degradation III 4 2 1
salidroside biosynthesis 4 2 1
L-tyrosine degradation III 4 2 1
superpathway of L-isoleucine biosynthesis I 13 13 3
L-arginine biosynthesis II (acetyl cycle) 10 10 2
ethanolamine utilization 5 5 1
L-ornithine biosynthesis I 5 5 1
pyruvate fermentation to isobutanol (engineered) 5 4 1
acetylene degradation (anaerobic) 5 4 1
protein S-nitrosylation and denitrosylation 5 3 1
(S)-propane-1,2-diol degradation 5 3 1
phenylethanol biosynthesis 5 2 1
superpathway of branched chain amino acid biosynthesis 17 17 3
peptido-conjugates in tissue regeneration biosynthesis 17 8 3
superpathway of L-threonine metabolism 18 14 3
γ-glutamyl cycle 6 4 1
leukotriene biosynthesis 6 2 1
noradrenaline and adrenaline degradation 13 8 2
3-methylbutanol biosynthesis (engineered) 7 6 1
alginate biosynthesis II (bacterial) 7 6 1
serotonin degradation 7 4 1
superpathway of methylglyoxal degradation 8 5 1
butanol and isobutanol biosynthesis (engineered) 8 3 1
L-arginine biosynthesis I (via L-ornithine) 9 9 1
L-arginine biosynthesis III (via N-acetyl-L-citrulline) 9 8 1
superpathway of fermentation (Chlamydomonas reinhardtii) 9 5 1
superpathway of C1 compounds oxidation to CO2 12 5 1
superpathway of Clostridium acetobutylicum solventogenic fermentation 13 5 1
L-tryptophan degradation V (side chain pathway) 13 1 1
cyclosporin A biosynthesis 15 2 1
mixed acid fermentation 16 13 1
superpathway of arginine and polyamine biosynthesis 17 16 1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 7 1
heterolactic fermentation 18 14 1
superpathway of anaerobic sucrose degradation 19 14 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 13 1
superpathway of N-acetylneuraminate degradation 22 15 1