Experiment set2S913 for Pseudomonas aeruginosa MRSN321

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carbon source 10 mM Sodium octanoate

Group: carbon source
Media: Hans_Basal_Media + Sodium octanoate (10 mM), pH=7
Culturing: Paeruginosa_MRSN321_ML2, 96 deep-well block, Aerobic, at 30 (C), shaken=700 rpm
By: Hans and Ryan on 7/7/2025
Media components: 0.03 M PIPES sesquisodium salt, 0.1 g/L Potassium Chloride, 0.01 g/L Sodium Chloride, 0.01 g/L Calcium chloride dihydrate, 0.1 g/L Magnesium chloride hexahydrate, 0.1 g/L Sodium sulfate, 0.25 g/L Ammonium chloride, 0.1 g/L Disodium phosphate, DL vitamins (0.0002 mg/L biotin, 0.0002 mg/L Folic Acid, 0.001 mg/L Pyridoxine HCl, 0.0005 mg/L Riboflavin, 0.0005 mg/L Thiamine HCl, 0.0005 mg/L Nicotinic Acid, 0.0005 mg/L calcium pantothenate, 1e-05 mg/L Cyanocobalamin, 0.0005 mg/L 4-Aminobenzoic acid, 0.0005 mg/L Lipoic acid), Sulfur-free DL minerals (0.0003 g/L Magnesium chloride hexahydrate, 0.00015 g/L Nitrilotriacetic acid disodium salt, 0.0001 g/L Sodium Chloride, 5e-05 g/L Manganese (II) chloride tetrahydrate, 1e-05 g/L Cobalt chloride hexahydrate, 1.3e-05 g/L Zinc chloride, 1e-05 g/L Calcium chloride dihydrate, 1e-05 g/L Iron (II) chloride tetrahydrate, 2.5e-06 g/L Nickel (II) chloride hexahydrate, 2e-06 g/L Aluminum chloride hydrate, 1e-06 g/L copper (II) chloride dihydrate, 1e-06 g/L Boric Acid, 1e-06 g/L Sodium Molybdate Dihydrate, 3e-05 g/L Sodium selenite pentahydrate, 2.5e-05 g/L Sodium tungstate dihydrate)

Specific Phenotypes

For 4 genes in this experiment

For carbon source Sodium octanoate in Pseudomonas aeruginosa MRSN321

For carbon source Sodium octanoate across organisms

SEED Subsystems

Subsystem #Specific
Carboxysome 1
Cyanate hydrolysis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
CO2 fixation into oxaloacetate (anaplerotic) 2 2 1
acetoacetate degradation (to acetyl CoA) 2 1 1
5,6-dehydrokavain biosynthesis (engineered) 10 7 4
fatty acid salvage 6 6 2
benzoyl-CoA biosynthesis 3 3 1
cyanate degradation 3 3 1
ketolysis 3 3 1
polyhydroxybutanoate biosynthesis 3 1 1
oleate β-oxidation 35 30 11
2-methyl-branched fatty acid β-oxidation 14 10 4
(2S)-ethylmalonyl-CoA biosynthesis 4 2 1
valproate β-oxidation 9 6 2
adipate degradation 5 5 1
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) 5 4 1
4-hydroxybenzoate biosynthesis III (plants) 5 4 1
glutaryl-CoA degradation 5 3 1
fatty acid β-oxidation II (plant peroxisome) 5 3 1
ketogenesis 5 3 1
9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) 10 4 2
fatty acid β-oxidation VII (yeast peroxisome) 5 2 1
ethylbenzene degradation (anaerobic) 5 1 1
pyruvate fermentation to acetone 5 1 1
isopropanol biosynthesis (engineered) 5 1 1
pyruvate fermentation to hexanol (engineered) 11 7 2
pyruvate fermentation to butanol II (engineered) 6 4 1
L-isoleucine degradation I 6 4 1
propanoate fermentation to 2-methylbutanoate 6 3 1
4-ethylphenol degradation (anaerobic) 6 2 1
10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) 6 1 1
jasmonic acid biosynthesis 19 4 3
fatty acid β-oxidation I (generic) 7 5 1
acetyl-CoA fermentation to butanoate 7 4 1
fatty acid β-oxidation VI (mammalian peroxisome) 7 4 1
C4 photosynthetic carbon assimilation cycle, NADP-ME type 7 4 1
pyruvate fermentation to butanoate 7 3 1
mevalonate pathway I (eukaryotes and bacteria) 7 1 1
mevalonate pathway II (haloarchaea) 7 1 1
2-deoxy-D-ribose degradation II 8 4 1
pyruvate fermentation to butanol I 8 3 1
2-methylpropene degradation 8 2 1
mevalonate pathway IV (archaea) 8 1 1
mevalonate pathway III (Thermoplasma) 8 1 1
isoprene biosynthesis II (engineered) 8 1 1
androstenedione degradation I (aerobic) 25 6 3
4-oxopentanoate degradation 9 6 1
superpathway of Clostridium acetobutylicum acidogenic fermentation 9 5 1
3-hydroxypropanoate/4-hydroxybutanate cycle 18 8 2
benzoate biosynthesis I (CoA-dependent, β-oxidative) 9 4 1
superpathway of testosterone and androsterone degradation 28 6 3
L-glutamate degradation V (via hydroxyglutarate) 10 6 1
3-phenylpropanoate degradation 10 5 1
superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) 10 4 1
L-lysine fermentation to acetate and butanoate 10 3 1
methyl tert-butyl ether degradation 10 3 1
superpathway of cholesterol degradation I (cholesterol oxidase) 42 8 4
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 8 1
(8E,10E)-dodeca-8,10-dienol biosynthesis 11 5 1
ethylmalonyl-CoA pathway 11 2 1
superpathway of cholesterol degradation II (cholesterol dehydrogenase) 47 9 4
L-glutamate degradation VII (to butanoate) 12 3 1
10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) 12 2 1
10-cis-heptadecenoyl-CoA degradation (yeast) 12 2 1
3-hydroxypropanoate cycle 13 5 1
superpathway of Clostridium acetobutylicum solventogenic fermentation 13 5 1
glyoxylate assimilation 13 4 1
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) 13 2 1
androstenedione degradation II (anaerobic) 27 4 2
superpathway of glyoxylate cycle and fatty acid degradation 14 12 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 9 1
docosahexaenoate biosynthesis III (6-desaturase, mammals) 14 2 1
L-tryptophan degradation III (eukaryotic) 15 6 1
glycerol degradation to butanol 16 9 1
crotonate fermentation (to acetate and cyclohexane carboxylate) 16 4 1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 7 1
benzoate fermentation (to acetate and cyclohexane carboxylate) 17 4 1
cholesterol degradation to androstenedione I (cholesterol oxidase) 17 2 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 9 1
superpathway of the 3-hydroxypropanoate cycle 18 5 1
toluene degradation VI (anaerobic) 18 4 1
sitosterol degradation to androstenedione 18 1 1
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) 22 3 1
superpathway of cholesterol degradation III (oxidase) 49 5 2
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 19 1
platensimycin biosynthesis 26 7 1
superpathway of ergosterol biosynthesis I 26 3 1
1-butanol autotrophic biosynthesis (engineered) 27 19 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 21 2
superpathway of cholesterol biosynthesis 38 3 1
superpathway of L-lysine degradation 43 15 1