Experiment set2S887 for Pseudomonas aeruginosa MRSN321

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carbon source 10 mM L-tyrosine disodium salt

Group: carbon source
Media: Hans_Basal_Media + L-tyrosine disodium salt (10 mM), pH=7
Culturing: Paeruginosa_MRSN321_ML2, 96 deep-well block, Aerobic, at 30 (C), shaken=700 rpm
By: Hans and Ryan on 7/7/2025
Media components: 0.03 M PIPES sesquisodium salt, 0.1 g/L Potassium Chloride, 0.01 g/L Sodium Chloride, 0.01 g/L Calcium chloride dihydrate, 0.1 g/L Magnesium chloride hexahydrate, 0.1 g/L Sodium sulfate, 0.25 g/L Ammonium chloride, 0.1 g/L Disodium phosphate, DL vitamins (0.0002 mg/L biotin, 0.0002 mg/L Folic Acid, 0.001 mg/L Pyridoxine HCl, 0.0005 mg/L Riboflavin, 0.0005 mg/L Thiamine HCl, 0.0005 mg/L Nicotinic Acid, 0.0005 mg/L calcium pantothenate, 1e-05 mg/L Cyanocobalamin, 0.0005 mg/L 4-Aminobenzoic acid, 0.0005 mg/L Lipoic acid), Sulfur-free DL minerals (0.0003 g/L Magnesium chloride hexahydrate, 0.00015 g/L Nitrilotriacetic acid disodium salt, 0.0001 g/L Sodium Chloride, 5e-05 g/L Manganese (II) chloride tetrahydrate, 1e-05 g/L Cobalt chloride hexahydrate, 1.3e-05 g/L Zinc chloride, 1e-05 g/L Calcium chloride dihydrate, 1e-05 g/L Iron (II) chloride tetrahydrate, 2.5e-06 g/L Nickel (II) chloride hexahydrate, 2e-06 g/L Aluminum chloride hydrate, 1e-06 g/L copper (II) chloride dihydrate, 1e-06 g/L Boric Acid, 1e-06 g/L Sodium Molybdate Dihydrate, 3e-05 g/L Sodium selenite pentahydrate, 2.5e-05 g/L Sodium tungstate dihydrate)

Specific Phenotypes

For 6 genes in this experiment

For carbon source L-tyrosine disodium salt in Pseudomonas aeruginosa MRSN321

For carbon source L-tyrosine disodium salt across organisms

SEED Subsystems

Subsystem #Specific
Aromatic amino acid degradation 3
Homogentisate pathway of aromatic compound degradation 3
Plastoquinone Biosynthesis 1
Tocopherol Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
3-(4-hydroxyphenyl)pyruvate biosynthesis 1 1 1
L-tyrosine degradation I 5 5 3
L-phenylalanine biosynthesis III (cytosolic, plants) 2 2 1
atromentin biosynthesis 2 1 1
L-tyrosine degradation II 2 1 1
superpathway of plastoquinol biosynthesis 5 2 2
L-phenylalanine biosynthesis I 3 3 1
L-tyrosine biosynthesis I 3 3 1
L-phenylalanine degradation II (anaerobic) 3 2 1
plastoquinol-9 biosynthesis I 3 1 1
L-tyrosine degradation IV (to 4-methylphenol) 3 1 1
L-tyrosine degradation III 4 2 1
L-phenylalanine degradation III 4 2 1
L-phenylalanine degradation IV (mammalian, via side chain) 9 4 2
L-tyrosine degradation V (reductive Stickland reaction) 5 1 1
4-hydroxybenzoate biosynthesis I (eukaryotes) 5 1 1
L-phenylalanine degradation VI (reductive Stickland reaction) 5 1 1
vitamin E biosynthesis (tocopherols) 7 1 1
superpathway of aromatic amino acid biosynthesis 18 18 2
superpathway of L-phenylalanine biosynthesis 10 10 1
superpathway of L-tyrosine biosynthesis 10 10 1
rosmarinic acid biosynthesis I 10 2 1
(S)-reticuline biosynthesis I 11 1 1
tropane alkaloids biosynthesis 11 1 1
superpathway of rosmarinic acid biosynthesis 14 2 1
superpathway of hyoscyamine (atropine) and scopolamine biosynthesis 16 3 1
anaerobic aromatic compound degradation (Thauera aromatica) 27 4 1
superpathway of chorismate metabolism 59 45 2