Experiment set2S877 for Pseudomonas aeruginosa MRSN321

Compare to:

carbon source 10 mM Choline chloride

Group: carbon source
Media: Hans_Basal_Media + Choline chloride (10 mM), pH=7
Culturing: Paeruginosa_MRSN321_ML2, 96 deep-well block, Aerobic, at 30 (C), shaken=700 rpm
By: Hans and Ryan on 7/7/2025
Media components: 0.03 M PIPES sesquisodium salt, 0.1 g/L Potassium Chloride, 0.01 g/L Sodium Chloride, 0.01 g/L Calcium chloride dihydrate, 0.1 g/L Magnesium chloride hexahydrate, 0.1 g/L Sodium sulfate, 0.25 g/L Ammonium chloride, 0.1 g/L Disodium phosphate, DL vitamins (0.0002 mg/L biotin, 0.0002 mg/L Folic Acid, 0.001 mg/L Pyridoxine HCl, 0.0005 mg/L Riboflavin, 0.0005 mg/L Thiamine HCl, 0.0005 mg/L Nicotinic Acid, 0.0005 mg/L calcium pantothenate, 1e-05 mg/L Cyanocobalamin, 0.0005 mg/L 4-Aminobenzoic acid, 0.0005 mg/L Lipoic acid), Sulfur-free DL minerals (0.0003 g/L Magnesium chloride hexahydrate, 0.00015 g/L Nitrilotriacetic acid disodium salt, 0.0001 g/L Sodium Chloride, 5e-05 g/L Manganese (II) chloride tetrahydrate, 1e-05 g/L Cobalt chloride hexahydrate, 1.3e-05 g/L Zinc chloride, 1e-05 g/L Calcium chloride dihydrate, 1e-05 g/L Iron (II) chloride tetrahydrate, 2.5e-06 g/L Nickel (II) chloride hexahydrate, 2e-06 g/L Aluminum chloride hydrate, 1e-06 g/L copper (II) chloride dihydrate, 1e-06 g/L Boric Acid, 1e-06 g/L Sodium Molybdate Dihydrate, 3e-05 g/L Sodium selenite pentahydrate, 2.5e-05 g/L Sodium tungstate dihydrate)

Specific Phenotypes

For 15 genes in this experiment

For carbon source Choline chloride in Pseudomonas aeruginosa MRSN321

For carbon source Choline chloride across organisms

SEED Subsystems

Subsystem #Specific
Choline and Betaine Uptake and Betaine Biosynthesis 8
Cyanophycin Metabolism 2
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Methionine Biosynthesis 1
Pyrroloquinoline Quinone biosynthesis 1
Threonine and Homoserine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-asparagine biosynthesis I 1 1 1
L-glutamine degradation I 1 1 1
cyanophycin metabolism 6 5 4
L-threonine biosynthesis 2 2 1
glycine betaine biosynthesis II (Gram-positive bacteria) 2 2 1
glycine betaine biosynthesis I (Gram-negative bacteria) 2 2 1
choline degradation I 2 2 1
L-glutamate biosynthesis I 2 2 1
superpathway of L-asparagine biosynthesis 2 1 1
glycine betaine degradation III 7 7 3
ammonia assimilation cycle III 3 3 1
choline-O-sulfate degradation 3 3 1
L-asparagine biosynthesis III (tRNA-dependent) 4 4 1
glycine betaine degradation I 8 6 2
glutaminyl-tRNAgln biosynthesis via transamidation 4 3 1
creatinine degradation I 4 1 1
creatinine degradation II 5 1 1
peptido-conjugates in tissue regeneration biosynthesis 17 8 3
superpathway of L-threonine biosynthesis 6 6 1
L-methionine biosynthesis II 6 5 1
leukotriene biosynthesis 6 2 1
L-glutamate and L-glutamine biosynthesis 7 6 1
L-citrulline biosynthesis 8 6 1
gliotoxin biosynthesis 9 2 1
superpathway of L-citrulline metabolism 12 8 1
superpathway of L-isoleucine biosynthesis I 13 13 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 16 1
aspartate superpathway 25 22 1
arachidonate metabolites biosynthesis 74 5 1