Experiment set2S869 for Pseudomonas aeruginosa MRSN321

Compare to:

carbon source 10 mM L-Glutamic acid monopotassium salt monohydrate

Group: carbon source
Media: Hans_Basal_Media + L-Glutamic acid monopotassium salt monohydrate (10 mM), pH=7
Culturing: Paeruginosa_MRSN321_ML2, 96 deep-well block, Aerobic, at 30 (C), shaken=700 rpm
By: Hans and Ryan on 7/7/2025
Media components: 0.03 M PIPES sesquisodium salt, 0.1 g/L Potassium Chloride, 0.01 g/L Sodium Chloride, 0.01 g/L Calcium chloride dihydrate, 0.1 g/L Magnesium chloride hexahydrate, 0.1 g/L Sodium sulfate, 0.25 g/L Ammonium chloride, 0.1 g/L Disodium phosphate, DL vitamins (0.0002 mg/L biotin, 0.0002 mg/L Folic Acid, 0.001 mg/L Pyridoxine HCl, 0.0005 mg/L Riboflavin, 0.0005 mg/L Thiamine HCl, 0.0005 mg/L Nicotinic Acid, 0.0005 mg/L calcium pantothenate, 1e-05 mg/L Cyanocobalamin, 0.0005 mg/L 4-Aminobenzoic acid, 0.0005 mg/L Lipoic acid), Sulfur-free DL minerals (0.0003 g/L Magnesium chloride hexahydrate, 0.00015 g/L Nitrilotriacetic acid disodium salt, 0.0001 g/L Sodium Chloride, 5e-05 g/L Manganese (II) chloride tetrahydrate, 1e-05 g/L Cobalt chloride hexahydrate, 1.3e-05 g/L Zinc chloride, 1e-05 g/L Calcium chloride dihydrate, 1e-05 g/L Iron (II) chloride tetrahydrate, 2.5e-06 g/L Nickel (II) chloride hexahydrate, 2e-06 g/L Aluminum chloride hydrate, 1e-06 g/L copper (II) chloride dihydrate, 1e-06 g/L Boric Acid, 1e-06 g/L Sodium Molybdate Dihydrate, 3e-05 g/L Sodium selenite pentahydrate, 2.5e-05 g/L Sodium tungstate dihydrate)

Specific Phenotypes

For 1 genes in this experiment

For carbon source L-Glutamic acid monopotassium salt monohydrate in Pseudomonas aeruginosa MRSN321

For carbon source L-Glutamic acid monopotassium salt monohydrate across organisms

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-alanine biosynthesis II 1 1 1
L-alanine degradation III 1 1 1
L-alanine degradation V (oxidative Stickland reaction) 2 1 1
L-alanine degradation II (to D-lactate) 3 3 1
pyruvate fermentation to acetate and alanine 3 1 1
superpathway of L-alanine biosynthesis 4 4 1
L-alanine degradation VI (reductive Stickland reaction) 6 2 1
anaerobic energy metabolism (invertebrates, cytosol) 7 5 1
superpathway of L-alanine fermentation (Stickland reaction) 9 4 1
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 8 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 9 1
superpathway of anaerobic energy metabolism (invertebrates) 17 11 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 21 1