Experiment set2S867 for Pseudomonas aeruginosa MRSN321
carbon source 10 mM L-Arginine
Group: carbon sourceMedia: Hans_Basal_Media + L-Arginine (10 mM), pH=7
Culturing: Paeruginosa_MRSN321_ML2, 96 deep-well block, Aerobic, at 30 (C), shaken=700 rpm
By: Hans and Ryan on 7/7/2025
Media components: 0.03 M PIPES sesquisodium salt, 0.1 g/L Potassium Chloride, 0.01 g/L Sodium Chloride, 0.01 g/L Calcium chloride dihydrate, 0.1 g/L Magnesium chloride hexahydrate, 0.1 g/L Sodium sulfate, 0.25 g/L Ammonium chloride, 0.1 g/L Disodium phosphate, DL vitamins (0.0002 mg/L biotin, 0.0002 mg/L Folic Acid, 0.001 mg/L Pyridoxine HCl, 0.0005 mg/L Riboflavin, 0.0005 mg/L Thiamine HCl, 0.0005 mg/L Nicotinic Acid, 0.0005 mg/L calcium pantothenate, 1e-05 mg/L Cyanocobalamin, 0.0005 mg/L 4-Aminobenzoic acid, 0.0005 mg/L Lipoic acid), Sulfur-free DL minerals (0.0003 g/L Magnesium chloride hexahydrate, 0.00015 g/L Nitrilotriacetic acid disodium salt, 0.0001 g/L Sodium Chloride, 5e-05 g/L Manganese (II) chloride tetrahydrate, 1e-05 g/L Cobalt chloride hexahydrate, 1.3e-05 g/L Zinc chloride, 1e-05 g/L Calcium chloride dihydrate, 1e-05 g/L Iron (II) chloride tetrahydrate, 2.5e-06 g/L Nickel (II) chloride hexahydrate, 2e-06 g/L Aluminum chloride hydrate, 1e-06 g/L copper (II) chloride dihydrate, 1e-06 g/L Boric Acid, 1e-06 g/L Sodium Molybdate Dihydrate, 3e-05 g/L Sodium selenite pentahydrate, 2.5e-05 g/L Sodium tungstate dihydrate)
Specific Phenotypes
For 8 genes in this experiment
For carbon source L-Arginine in Pseudomonas aeruginosa MRSN321
For carbon source L-Arginine across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Arginine and proline metabolism
- Urea cycle and metabolism of amino groups
- Glutamate metabolism
- Glycine, serine and threonine metabolism
- Valine, leucine and isoleucine biosynthesis
- Nitrogen metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-glutamate degradation I | 1 | 1 | 1 |
| L-methionine degradation II | 3 | 2 | 2 |
| L-threonine degradation I | 6 | 5 | 3 |
| L-threonine degradation V | 2 | 1 | 1 |
| L-isoleucine biosynthesis I (from threonine) | 7 | 7 | 3 |
| putrescine biosynthesis II | 3 | 3 | 1 |
| L-alanine degradation II (to D-lactate) | 3 | 3 | 1 |
| L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway) | 3 | 3 | 1 |
| ethene biosynthesis IV (engineered) | 3 | 1 | 1 |
| superpathway of L-isoleucine biosynthesis I | 13 | 13 | 3 |
| hypoglycin biosynthesis | 14 | 4 | 3 |
| superpathway of branched chain amino acid biosynthesis | 17 | 17 | 3 |
| superpathway of L-threonine metabolism | 18 | 14 | 3 |
| L-glutamate degradation XI (reductive Stickland reaction) | 7 | 3 | 1 |
| 4-aminobutanoate degradation V | 7 | 2 | 1 |
| superpathway of polyamine biosynthesis II | 8 | 6 | 1 |
| L-glutamate degradation V (via hydroxyglutarate) | 10 | 6 | 1 |
| cyclosporin A biosynthesis | 15 | 2 | 1 |
| methylaspartate cycle | 19 | 10 | 1 |