Experiment set2S828 for Pseudomonas aeruginosa MRSN321

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carbon source 10 mM L-ascorbic acid

Group: carbon source
Media: Hans_Basal_Media + L-ascorbic acid (10 mM), pH=7
Culturing: Paeruginosa_MRSN321_ML2, 96 deep-well block, Aerobic, at 30 (C), shaken=700 rpm
By: Hans and Ryan on 7/7/2025
Media components: 0.03 M PIPES sesquisodium salt, 0.1 g/L Potassium Chloride, 0.01 g/L Sodium Chloride, 0.01 g/L Calcium chloride dihydrate, 0.1 g/L Magnesium chloride hexahydrate, 0.1 g/L Sodium sulfate, 0.25 g/L Ammonium chloride, 0.1 g/L Disodium phosphate, DL vitamins (0.0002 mg/L biotin, 0.0002 mg/L Folic Acid, 0.001 mg/L Pyridoxine HCl, 0.0005 mg/L Riboflavin, 0.0005 mg/L Thiamine HCl, 0.0005 mg/L Nicotinic Acid, 0.0005 mg/L calcium pantothenate, 1e-05 mg/L Cyanocobalamin, 0.0005 mg/L 4-Aminobenzoic acid, 0.0005 mg/L Lipoic acid), Sulfur-free DL minerals (0.0003 g/L Magnesium chloride hexahydrate, 0.00015 g/L Nitrilotriacetic acid disodium salt, 0.0001 g/L Sodium Chloride, 5e-05 g/L Manganese (II) chloride tetrahydrate, 1e-05 g/L Cobalt chloride hexahydrate, 1.3e-05 g/L Zinc chloride, 1e-05 g/L Calcium chloride dihydrate, 1e-05 g/L Iron (II) chloride tetrahydrate, 2.5e-06 g/L Nickel (II) chloride hexahydrate, 2e-06 g/L Aluminum chloride hydrate, 1e-06 g/L copper (II) chloride dihydrate, 1e-06 g/L Boric Acid, 1e-06 g/L Sodium Molybdate Dihydrate, 3e-05 g/L Sodium selenite pentahydrate, 2.5e-05 g/L Sodium tungstate dihydrate)

Specific Phenotypes

For 10 genes in this experiment

For carbon source L-ascorbic acid in Pseudomonas aeruginosa MRSN321

For carbon source L-ascorbic acid across organisms

SEED Subsystems

Subsystem #Specific
Molybdenum cofactor biosynthesis 2
Arginine and Ornithine Degradation 1
D-galactarate, D-glucarate and D-glycerate catabolism 1
Glutathione: Non-redox reactions 1
Glycine and Serine Utilization 1
Glycine cleavage system 1
Glycolate, glyoxylate interconversions 1
Methylglyoxal Metabolism 1
Murein hydrolase regulation and cell death 1
Photorespiration (oxidative C2 cycle) 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
bis(guanylyl molybdenum cofactor) biosynthesis 2 2 2
guanylyl molybdenum cofactor biosynthesis 1 1 1
bis(guanylyl tungstenpterin) cofactor biosynthesis 1 1 1
L-lyxonate degradation 3 3 2
glycine cleavage 3 3 1
glycine biosynthesis II 3 3 1
methylglyoxal degradation I 3 2 1
methylglyoxal degradation VIII 3 2 1
L-arabinose degradation III 6 3 1
superpathway of methylglyoxal degradation 8 5 1
superpathway of pentose and pentitol degradation 42 11 1