Experiment set2S817 for Pseudomonas aeruginosa MRSN321
carbon source 10 mM Sodium octanoate
Group: carbon sourceMedia: Hans_Basal_Media + Sodium octanoate (10 mM), pH=7
Culturing: Paeruginosa_MRSN321_ML2, 96 deep-well block, Aerobic, at 30 (C), shaken=700 rpm
By: Hans and Ryan on 7/7/2025
Media components: 0.03 M PIPES sesquisodium salt, 0.1 g/L Potassium Chloride, 0.01 g/L Sodium Chloride, 0.01 g/L Calcium chloride dihydrate, 0.1 g/L Magnesium chloride hexahydrate, 0.1 g/L Sodium sulfate, 0.25 g/L Ammonium chloride, 0.1 g/L Disodium phosphate, DL vitamins (0.0002 mg/L biotin, 0.0002 mg/L Folic Acid, 0.001 mg/L Pyridoxine HCl, 0.0005 mg/L Riboflavin, 0.0005 mg/L Thiamine HCl, 0.0005 mg/L Nicotinic Acid, 0.0005 mg/L calcium pantothenate, 1e-05 mg/L Cyanocobalamin, 0.0005 mg/L 4-Aminobenzoic acid, 0.0005 mg/L Lipoic acid), Sulfur-free DL minerals (0.0003 g/L Magnesium chloride hexahydrate, 0.00015 g/L Nitrilotriacetic acid disodium salt, 0.0001 g/L Sodium Chloride, 5e-05 g/L Manganese (II) chloride tetrahydrate, 1e-05 g/L Cobalt chloride hexahydrate, 1.3e-05 g/L Zinc chloride, 1e-05 g/L Calcium chloride dihydrate, 1e-05 g/L Iron (II) chloride tetrahydrate, 2.5e-06 g/L Nickel (II) chloride hexahydrate, 2e-06 g/L Aluminum chloride hydrate, 1e-06 g/L copper (II) chloride dihydrate, 1e-06 g/L Boric Acid, 1e-06 g/L Sodium Molybdate Dihydrate, 3e-05 g/L Sodium selenite pentahydrate, 2.5e-05 g/L Sodium tungstate dihydrate)
Specific Phenotypes
For 5 genes in this experiment
For carbon source Sodium octanoate in Pseudomonas aeruginosa MRSN321
For carbon source Sodium octanoate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Carboxysome | 1 |
| Cyanate hydrolysis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fatty acid metabolism
- Valine, leucine and isoleucine degradation
- Geraniol degradation
- 1- and 2-Methylnaphthalene degradation
- Benzoate degradation via CoA ligation
- Brassinosteroid biosynthesis
- Nitrogen metabolism
- Caprolactam degradation
- Biosynthesis of terpenoids and steroids
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| CO2 fixation into oxaloacetate (anaplerotic) | 2 | 2 | 1 |
| cyanate degradation | 3 | 3 | 1 |
| adipate degradation | 5 | 5 | 1 |
| fatty acid salvage | 6 | 6 | 1 |
| C4 photosynthetic carbon assimilation cycle, NADP-ME type | 7 | 4 | 1 |
| C4 photosynthetic carbon assimilation cycle, NAD-ME type | 11 | 8 | 1 |
| oleate β-oxidation | 35 | 30 | 3 |
| 3-hydroxypropanoate cycle | 13 | 5 | 1 |
| glyoxylate assimilation | 13 | 4 | 1 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 10 | 1 |
| C4 photosynthetic carbon assimilation cycle, PEPCK type | 14 | 9 | 1 |
| gluconeogenesis II (Methanobacterium thermoautotrophicum) | 18 | 9 | 1 |
| 3-hydroxypropanoate/4-hydroxybutanate cycle | 18 | 8 | 1 |
| superpathway of the 3-hydroxypropanoate cycle | 18 | 5 | 1 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | 56 | 21 | 1 |