Experiment set2S786 for Pseudomonas aeruginosa MRSN321

carbon source 10 mM L-Pyroglutamic acid

Group: carbon source
Media: Hans_Basal_Media + L-Pyroglutamic acid (10 mM), pH=7
Culturing: Paeruginosa_MRSN321_ML2, 96 deep-well block, Aerobic, at 30 (C), shaken=700 rpm
By: Hans and Ryan on 7/7/2025
Media components: 0.03 M PIPES sesquisodium salt, 0.1 g/L Potassium Chloride, 0.01 g/L Sodium Chloride, 0.01 g/L Calcium chloride dihydrate, 0.1 g/L Magnesium chloride hexahydrate, 0.1 g/L Sodium sulfate, 0.25 g/L Ammonium chloride, 0.1 g/L Disodium phosphate, DL vitamins (0.0002 mg/L biotin, 0.0002 mg/L Folic Acid, 0.001 mg/L Pyridoxine HCl, 0.0005 mg/L Riboflavin, 0.0005 mg/L Thiamine HCl, 0.0005 mg/L Nicotinic Acid, 0.0005 mg/L calcium pantothenate, 1e-05 mg/L Cyanocobalamin, 0.0005 mg/L 4-Aminobenzoic acid, 0.0005 mg/L Lipoic acid), Sulfur-free DL minerals (0.0003 g/L Magnesium chloride hexahydrate, 0.00015 g/L Nitrilotriacetic acid disodium salt, 0.0001 g/L Sodium Chloride, 5e-05 g/L Manganese (II) chloride tetrahydrate, 1e-05 g/L Cobalt chloride hexahydrate, 1.3e-05 g/L Zinc chloride, 1e-05 g/L Calcium chloride dihydrate, 1e-05 g/L Iron (II) chloride tetrahydrate, 2.5e-06 g/L Nickel (II) chloride hexahydrate, 2e-06 g/L Aluminum chloride hydrate, 1e-06 g/L copper (II) chloride dihydrate, 1e-06 g/L Boric Acid, 1e-06 g/L Sodium Molybdate Dihydrate, 3e-05 g/L Sodium selenite pentahydrate, 2.5e-05 g/L Sodium tungstate dihydrate)

Specific Phenotypes

For 12 genes in this experiment

For carbon source L-Pyroglutamic acid in Pseudomonas aeruginosa MRSN321

For carbon source L-Pyroglutamic acid across organisms

SEED Subsystems

Subsystem	#Specific
Glutathione-dependent pathway of formaldehyde detoxification	2
D-ribose utilization	1
De Novo Pyrimidine Synthesis	1
LMPTP YwlE cluster	1
Rhamnose containing glycans	1
Transport of Zinc	1
dTDP-rhamnose synthesis	1
linker unit-arabinogalactan synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Methane metabolism
• Glycolysis / Gluconeogenesis
• Fatty acid metabolism
• Pyrimidine metabolism
• Glycine, serine and threonine metabolism
• Tyrosine metabolism
• Streptomycin biosynthesis
• Polyketide sugar unit biosynthesis
• 1- and 2-Methylnaphthalene degradation
• 3-Chloroacrylic acid degradation
• Retinol metabolism
• Metabolism of xenobiotics by cytochrome P450
• Drug metabolism - cytochrome P450

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pyrimidine nucleobases salvage I	1	1	1
acetaldehyde biosynthesis I	1	1	1
cis-cyclopropane fatty acid (CFA) biosynthesis	1	1	1
formaldehyde oxidation II (glutathione-dependent)	3	3	2
pyrimidine nucleobases salvage II	2	2	1
ethanol degradation I	2	2	1
pyruvate fermentation to ethanol II	2	1	1
sterculate biosynthesis	2	1	1
ethanol degradation II	3	3	1
L-isoleucine degradation II	3	2	1
methylglyoxal degradation I	3	2	1
pyruvate fermentation to ethanol III	3	2	1
pyruvate fermentation to ethanol I	3	2	1
methylglyoxal degradation VIII	3	2	1
L-valine degradation II	3	2	1
L-leucine degradation III	3	2	1
L-methionine degradation III	3	1	1
superpathway of pyrimidine nucleobases salvage	4	4	1
phytol degradation	4	3	1
salidroside biosynthesis	4	2	1
L-tyrosine degradation III	4	2	1
L-phenylalanine degradation III	4	2	1
dTDP-β-L-rhamnose biosynthesis	5	5	1
ethanolamine utilization	5	5	1
pyruvate fermentation to isobutanol (engineered)	5	4	1
acetylene degradation (anaerobic)	5	4	1
(S)-propane-1,2-diol degradation	5	3	1
protein S-nitrosylation and denitrosylation	5	3	1
phenylethanol biosynthesis	5	2	1
superpathway of C1 compounds oxidation to CO2	12	5	2
noradrenaline and adrenaline degradation	13	8	2
3-methylbutanol biosynthesis (engineered)	7	6	1
serotonin degradation	7	4	1
superpathway of methylglyoxal degradation	8	5	1
butanol and isobutanol biosynthesis (engineered)	8	3	1
superpathway of fermentation (Chlamydomonas reinhardtii)	9	5	1
superpathway of pyrimidine ribonucleosides salvage	10	6	1
O-antigen building blocks biosynthesis (E. coli)	11	10	1
superpathway of Clostridium acetobutylicum solventogenic fermentation	13	5	1
L-tryptophan degradation V (side chain pathway)	13	1	1
mixed acid fermentation	16	13	1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation	17	7	1
heterolactic fermentation	18	14	1
superpathway of anaerobic sucrose degradation	19	14	1
hexitol fermentation to lactate, formate, ethanol and acetate	19	13	1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis	19	5	1
superpathway of N-acetylneuraminate degradation	22	15	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	12	1
mycolate biosynthesis	205	20	3
superpathway of mycolate biosynthesis	239	21	3