Experiment set2S785 for Pseudomonas aeruginosa MRSN321
carbon source 10 mM Spermidine trihydrochloride
Group: carbon sourceMedia: Hans_Basal_Media + Spermidine trihydrochloride (10 mM), pH=7
Culturing: Paeruginosa_MRSN321_ML2, 96 deep-well block, Aerobic, at 30 (C), shaken=700 rpm
By: Hans and Ryan on 7/7/2025
Media components: 0.03 M PIPES sesquisodium salt, 0.1 g/L Potassium Chloride, 0.01 g/L Sodium Chloride, 0.01 g/L Calcium chloride dihydrate, 0.1 g/L Magnesium chloride hexahydrate, 0.1 g/L Sodium sulfate, 0.25 g/L Ammonium chloride, 0.1 g/L Disodium phosphate, DL vitamins (0.0002 mg/L biotin, 0.0002 mg/L Folic Acid, 0.001 mg/L Pyridoxine HCl, 0.0005 mg/L Riboflavin, 0.0005 mg/L Thiamine HCl, 0.0005 mg/L Nicotinic Acid, 0.0005 mg/L calcium pantothenate, 1e-05 mg/L Cyanocobalamin, 0.0005 mg/L 4-Aminobenzoic acid, 0.0005 mg/L Lipoic acid), Sulfur-free DL minerals (0.0003 g/L Magnesium chloride hexahydrate, 0.00015 g/L Nitrilotriacetic acid disodium salt, 0.0001 g/L Sodium Chloride, 5e-05 g/L Manganese (II) chloride tetrahydrate, 1e-05 g/L Cobalt chloride hexahydrate, 1.3e-05 g/L Zinc chloride, 1e-05 g/L Calcium chloride dihydrate, 1e-05 g/L Iron (II) chloride tetrahydrate, 2.5e-06 g/L Nickel (II) chloride hexahydrate, 2e-06 g/L Aluminum chloride hydrate, 1e-06 g/L copper (II) chloride dihydrate, 1e-06 g/L Boric Acid, 1e-06 g/L Sodium Molybdate Dihydrate, 3e-05 g/L Sodium selenite pentahydrate, 2.5e-05 g/L Sodium tungstate dihydrate)
Specific Phenotypes
For 14 genes in this experiment
For carbon source Spermidine trihydrochloride in Pseudomonas aeruginosa MRSN321
For carbon source Spermidine trihydrochloride across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Polyamine Metabolism | 5 |
| Pyruvate Alanine Serine Interconversions | 2 |
| DNA-binding regulatory proteins, strays | 1 |
| Isobutyryl-CoA to Propionyl-CoA Module | 1 |
| Valine degradation | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Valine, leucine and isoleucine degradation
- Arginine and proline metabolism
- Nucleotide sugars metabolism
- Propanoate metabolism
- Urea cycle and metabolism of amino groups
- Puromycin biosynthesis
- Alanine and aspartate metabolism
- Methionine metabolism
- Lysine biosynthesis
- Lysine degradation
- beta-Alanine metabolism
- Aminophosphonate metabolism
- Inositol phosphate metabolism
- alpha-Linolenic acid metabolism
- Trinitrotoluene degradation
- Porphyrin and chlorophyll metabolism
- Carotenoid biosynthesis - General
- Caprolactam degradation
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| β-alanine degradation II | 2 | 2 | 2 |
| putrescine degradation V | 2 | 2 | 1 |
| β-alanine degradation I | 2 | 1 | 1 |
| putrescine degradation II | 4 | 2 | 1 |
| acrylate degradation I | 5 | 2 | 1 |
| propanoyl-CoA degradation II | 5 | 2 | 1 |
| β-alanine biosynthesis II | 6 | 4 | 1 |
| myo-inositol degradation I | 7 | 1 | 1 |
| 2,4-dinitrotoluene degradation | 7 | 1 | 1 |
| superpathway of ornithine degradation | 8 | 5 | 1 |
| L-valine degradation I | 8 | 5 | 1 |
| superpathway of coenzyme A biosynthesis II (plants) | 10 | 8 | 1 |
| myo-, chiro- and scyllo-inositol degradation | 10 | 1 | 1 |
| superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation | 11 | 6 | 1 |
| superpathway of L-arginine and L-ornithine degradation | 13 | 8 | 1 |