Experiment set2S700 for Escherichia coli ECOR38

Sodium-DL-Lactate

Group: carbon source
Media: M9_plus_Sodium-DL-Lactate + Sodium-DL-Lactate (20 mM)
Culturing: Ecoli_ECOR38_ML2, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 12/6/24

Specific Phenotypes

For 3 genes in this experiment

For carbon source Sodium-DL-Lactate in Escherichia coli ECOR38

For carbon source Sodium-DL-Lactate across organisms

SEED Subsystems

Subsystem	#Specific
Acetoin, butanediol metabolism	1
Branched-Chain Amino Acid Biosynthesis	1
Fructose utilization	1
Glycolysis and Gluconeogenesis	1
Glycolysis and Gluconeogenesis, including Archaeal enzymes	1
Pyruvate metabolism I: anaplerotic reactions, PEP	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway
• Fructose and mannose metabolism
• Valine, leucine and isoleucine biosynthesis
• Lipopolysaccharide biosynthesis
• Pyruvate metabolism
• Butanoate metabolism
• C5-Branched dibasic acid metabolism
• Reductive carboxylate cycle (CO2 fixation)
• Nicotinate and nicotinamide metabolism
• Pantothenate and CoA biosynthesis
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
fructose degradation	1	1	1
pyruvate fermentation to (R)-acetoin II	2	1	1
pyruvate fermentation to (S)-acetoin	3	3	1
pyruvate fermentation to (R)-acetoin I	3	2	1
D-galactosamine and N-acetyl-D-galactosamine degradation	4	4	1
L-valine biosynthesis	4	4	1
galactitol degradation	5	5	1
pyruvate fermentation to isobutanol (engineered)	5	4	1
N-acetyl-D-galactosamine degradation	5	4	1
superpathway of (R,R)-butanediol biosynthesis	5	2	1
lactose degradation I	5	2	1
L-isoleucine biosynthesis IV	6	4	1
superpathway of 2,3-butanediol biosynthesis	6	3	1
L-isoleucine biosynthesis I (from threonine)	7	7	1
L-isoleucine biosynthesis III	7	5	1
L-isoleucine biosynthesis II	8	4	1
superpathway of branched chain amino acid biosynthesis	17	17	2
superpathway of hexitol degradation (bacteria)	18	18	2
glycolysis V (Pyrococcus)	10	7	1
glycolysis II (from fructose 6-phosphate)	11	11	1
superpathway of L-isoleucine biosynthesis I	13	13	1
gluconeogenesis I	13	13	1
glycolysis I (from glucose 6-phosphate)	13	13	1
superpathway of glycolysis and the Entner-Doudoroff pathway	17	17	1
superpathway of L-threonine metabolism	18	15	1
gluconeogenesis II (Methanobacterium thermoautotrophicum)	18	9	1
hexitol fermentation to lactate, formate, ethanol and acetate	19	19	1
superpathway of anaerobic sucrose degradation	19	17	1
superpathway of N-acetylneuraminate degradation	22	22	1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass	26	25	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	22	1