Experiment set2S569 for Bacteroides stercoris CC31F
Columbia 1 x carbon source complex media
Group: carbon source complex mediaMedia: Varel_Bryant_medium + 1x Columbia
Culturing: Bstercoris_CC31F_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/31/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Columbia 1x includes: 5 g/L Proteose Peptone, 10 g/L Tryptone, 5 g/L Yeast Extract, 3 g/L Beef Heart Infusion, 2.5 g/L D-Glucose, 5 g/L Sodium Chloride, 0.6 g/L Sodium carbonate, 0.02 g/L ammonium iron(II) sulfate hexahydrate, 0.1 g/L Magnesium sulfate, 0.1 g/L L-Cysteine hydrochloride monohydrate, 0.83 g/L Tris(hydroxymethyl)aminomethane, 2.86 g/L Tris hydrochloride, 0.005 g/L Hemin, 0.001 g/L Menadione
Specific Phenotypes
For 2 genes in this experiment
For carbon source complex media Columbia in Bacteroides stercoris CC31F
For carbon source complex media Columbia across organisms
Metabolic Maps
Color code by fitness: see overview map or list of maps.