Experiment set2S561 for Bacteroides stercoris CC31F
N-Acetyl-D-Glucosamine 10 mM carbon source
Group: carbon sourceMedia: Varel_Bryant_medium + N-Acetyl-D-Glucosamine (10 mM)
Culturing: Bstercoris_CC31F_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/31/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 2 genes in this experiment
For carbon source N-Acetyl-D-Glucosamine in Bacteroides stercoris CC31F
For carbon source N-Acetyl-D-Glucosamine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Chitin and N-acetylglucosamine utilization | 1 |
| Sialic Acid Metabolism | 1 |
| UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| N-acetylglucosamine degradation I | 2 | 2 | 1 |
| N-acetylglucosamine degradation II | 3 | 3 | 1 |
| superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation | 6 | 2 | 1 |
| UDP-N-acetyl-D-galactosamine biosynthesis II | 7 | 4 | 1 |
| chitin degradation I (archaea) | 7 | 2 | 1 |
| chitin derivatives degradation | 8 | 3 | 1 |
| superpathway of N-acetylneuraminate degradation | 22 | 15 | 1 |