Experiment set2S557 for Bacteroides stercoris CC31F

D-Galactose 10 mM carbon source

Group: carbon source
Media: Varel_Bryant_medium + D-Galactose (10 mM)
Culturing: Bstercoris_CC31F_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/31/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 1 genes in this experiment

For carbon source D-Galactose in Bacteroides stercoris CC31F

For carbon source D-Galactose across organisms

SEED Subsystems

Subsystem	#Specific
De Novo Pyrimidine Synthesis	1
Macromolecular synthesis operon	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pyrimidine metabolism
• Glutamate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamine degradation I	1	1	1
L-glutamate biosynthesis I	2	2	1
ammonia assimilation cycle III	3	3	1
L-citrulline degradation	3	2	1
L-arginine degradation V (arginine deiminase pathway)	4	2	1
L-asparagine biosynthesis III (tRNA-dependent)	4	1	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	1	1
UMP biosynthesis III	6	6	1
UMP biosynthesis II	6	6	1
UMP biosynthesis I	6	5	1
guadinomine B biosynthesis	13	2	2
L-glutamate and L-glutamine biosynthesis	7	5	1
L-citrulline biosynthesis	8	5	1
L-arginine biosynthesis III (via N-acetyl-L-citrulline)	9	9	1
L-arginine biosynthesis I (via L-ornithine)	9	8	1
superpathway of pyrimidine ribonucleotides de novo biosynthesis	9	7	1
L-arginine biosynthesis IV (archaea)	9	3	1
allantoin degradation IV (anaerobic)	9	2	1
L-arginine biosynthesis II (acetyl cycle)	10	8	1
superpathway of L-citrulline metabolism	12	7	1
superpathway of arginine and polyamine biosynthesis	17	10	1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis	18	12	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	38	1