Experiment set2S551 for Bacteroides stercoris CC31F

Compare to:

marine_broth_2216 1 x carbon source complex media

Group: carbon source complex media
Media: Varel_Bryant_medium + 1x marine_broth_2216
Culturing: Bstercoris_CC31F_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/31/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

marine_broth_2216 1x includes: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate

Specific Phenotypes

For 8 genes in this experiment

For carbon source complex media marine_broth_2216 in Bacteroides stercoris CC31F

For carbon source complex media marine_broth_2216 across organisms

SEED Subsystems

Subsystem #Specific
Choline and Betaine Uptake and Betaine Biosynthesis 3
Menaquinone and Phylloquinone Biosynthesis 1
Purine conversions 1
Pyruvate Alanine Serine Interconversions 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-alanine degradation IV 1 1 1
long-chain fatty acid activation 1 1 1
linoleate biosynthesis II (animals) 2 1 1
γ-linolenate biosynthesis II (animals) 2 1 1
3-methyl-branched fatty acid α-oxidation 6 2 2
oleate biosynthesis I (plants) 3 1 1
alkane biosynthesis II 3 1 1
phytol degradation 4 1 1
wax esters biosynthesis II 4 1 1
phosphatidylcholine acyl editing 4 1 1
long chain fatty acid ester synthesis (engineered) 4 1 1
sporopollenin precursors biosynthesis 18 4 4
sphingosine and sphingosine-1-phosphate metabolism 10 2 2
octane oxidation 5 1 1
stearate biosynthesis II (bacteria and plants) 6 4 1
stearate biosynthesis IV 6 3 1
fatty acid salvage 6 2 1
stearate biosynthesis I (animals) 6 1 1
6-gingerol analog biosynthesis (engineered) 6 1 1
2-carboxy-1,4-naphthoquinol biosynthesis 7 5 1
capsaicin biosynthesis 7 1 1
ceramide degradation by α-oxidation 7 1 1
arachidonate biosynthesis III (6-desaturase, mammals) 7 1 1
icosapentaenoate biosynthesis III (8-desaturase, mammals) 7 1 1
icosapentaenoate biosynthesis II (6-desaturase, mammals) 7 1 1
ceramide and sphingolipid recycling and degradation (yeast) 16 2 2
superpathway of demethylmenaquinol-8 biosynthesis I 9 7 1
superpathway of demethylmenaquinol-9 biosynthesis 9 6 1
superpathway of demethylmenaquinol-6 biosynthesis I 9 6 1
superpathway of menaquinol-8 biosynthesis I 10 8 1
superpathway of menaquinol-13 biosynthesis 10 7 1
superpathway of menaquinol-9 biosynthesis 10 7 1
superpathway of menaquinol-10 biosynthesis 10 7 1
superpathway of menaquinol-6 biosynthesis 10 7 1
superpathway of menaquinol-12 biosynthesis 10 7 1
superpathway of menaquinol-7 biosynthesis 10 7 1
superpathway of menaquinol-11 biosynthesis 10 7 1
suberin monomers biosynthesis 20 2 2
superpathway of fatty acid biosynthesis II (plant) 43 36 4
superpathway of phylloquinol biosynthesis 15 6 1
palmitate biosynthesis II (type II fatty acid synthase) 31 29 2
cutin biosynthesis 16 1 1
superpathway of fatty acids biosynthesis (E. coli) 53 47 2
palmitate biosynthesis III 29 21 1
oleate β-oxidation 35 4 1
superpathway of chorismate metabolism 59 38 1