Experiment set2S534 for Escherichia coli HS(pFamp)R (ATCC 700891)

K5

Group: phage
Media: LB_plus_SM_buffer + MOI 10
Culturing: Ecoli_HSF_ML10, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 12/4/24
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 1 genes in this experiment

For phage MOI 10 in Escherichia coli HS(pFamp)R (ATCC 700891)

For phage MOI 10 across organisms

SEED Subsystems

Subsystem	#Specific
Pyruvate metabolism I: anaplerotic reactions, PEP	1
Serine-glyoxylate cycle	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycolysis / Gluconeogenesis
• Citrate cycle (TCA cycle)
• Pyruvate metabolism
• Carbon fixation in photosynthetic organisms

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
gluconeogenesis I	13	12	1
superpathway of glyoxylate cycle and fatty acid degradation	14	11	1
C4 photosynthetic carbon assimilation cycle, PEPCK type	14	9	1