Experiment set2S527 for Bacteroides stercoris CC31F

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a-Cyclodextrin 2.5 mg/mL carbon source

Group: carbon source
Media: Varel_Bryant_medium + a-Cyclodextrin (2.5 mg/mL)
Culturing: Bstercoris_CC31F_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/31/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 24 genes in this experiment

For carbon source a-Cyclodextrin in Bacteroides stercoris CC31F

For carbon source a-Cyclodextrin across organisms

SEED Subsystems

Subsystem #Specific
Cysteine Biosynthesis 1
DNA Repair Base Excision 1
High affinity phosphate transporter and control of PHO regulon 1
Phosphate metabolism 1
Polyamine Metabolism 1
Potassium homeostasis 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1
Ribosomal protein S12p Asp methylthiotransferase 1
Type IV pilus 1
tRNA processing 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
C4 photosynthetic carbon assimilation cycle, NADP-ME type 7 4 2
methylgallate degradation 6 2 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 7 2
protocatechuate degradation I (meta-cleavage pathway) 8 3 1
superpathway of vanillin and vanillate degradation 10 3 1
syringate degradation 12 3 1
gluconeogenesis I 13 11 1