Experiment set2S526 for Bacteroides stercoris CC31F

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Rhamnogalacturonan from soy bean pectic fibre 2.5 mg/mL carbon source

Group: carbon source
Media: Varel_Bryant_medium + Rhamnogalacturonan from soy bean pectic fibre (2.5 mg/mL)
Culturing: Bstercoris_CC31F_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/31/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 11 genes in this experiment

For carbon source Rhamnogalacturonan from soy bean pectic fibre in Bacteroides stercoris CC31F

For carbon source Rhamnogalacturonan from soy bean pectic fibre across organisms

SEED Subsystems

Subsystem #Specific
Xylose utilization 3
Glycerol and Glycerol-3-phosphate Uptake and Utilization 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
High affinity phosphate transporter and control of PHO regulon 1
Phosphate metabolism 1
Purine conversions 1
Respiratory dehydrogenases 1 1
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
D-xylose degradation I 2 2 1
glycerol-3-phosphate shuttle 2 2 1
glycerophosphodiester degradation 2 2 1
nitrate reduction IX (dissimilatory) 2 1 1
xylitol degradation I 2 1 1
glycerol-3-phosphate to fumarate electron transfer 2 1 1
glycerol-3-phosphate to hydrogen peroxide electron transport 2 1 1
D-arabinitol degradation I 2 1 1
(1,4)-β-D-xylan degradation 2 1 1
polyphosphate metabolism 2 1 1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer 2 1 1
glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer 2 1 1
glycerol degradation I 3 1 1
sn-glycerol 3-phosphate anaerobic respiration 3 1 1
glycerol and glycerophosphodiester degradation 4 2 1
superpathway of glucose and xylose degradation 17 16 1
superpathway of pentose and pentitol degradation 42 5 1