Experiment set2S509 for Bacteroides stercoris CC31F

Columbia 1 x carbon source complex media

Group: carbon source complex media
Media: Varel_Bryant_medium + 1x Columbia
Culturing: Bstercoris_CC31F_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/31/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Columbia 1x includes: 5 g/L Proteose Peptone, 10 g/L Tryptone, 5 g/L Yeast Extract, 3 g/L Beef Heart Infusion, 2.5 g/L D-Glucose, 5 g/L Sodium Chloride, 0.6 g/L Sodium carbonate, 0.02 g/L ammonium iron(II) sulfate hexahydrate, 0.1 g/L Magnesium sulfate, 0.1 g/L L-Cysteine hydrochloride monohydrate, 0.83 g/L Tris(hydroxymethyl)aminomethane, 2.86 g/L Tris hydrochloride, 0.005 g/L Hemin, 0.001 g/L Menadione

Specific Phenotypes

For 6 genes in this experiment

For carbon source complex media Columbia in Bacteroides stercoris CC31F

For carbon source complex media Columbia across organisms

SEED Subsystems

Subsystem	#Specific
Multidrug Resistance Efflux Pumps	3
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	3
Phosphate metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.