Experiment set2S495 for Bacteroides stercoris CC31F

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D-Glucose 10 mM carbon source

Group: carbon source
Media: Varel_Bryant_medium + D-Glucose (10 mM)
Culturing: Bstercoris_CC31F_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/31/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 2 genes in this experiment

For carbon source D-Glucose in Bacteroides stercoris CC31F

For carbon source D-Glucose across organisms

SEED Subsystems

Subsystem #Specific
De Novo Purine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
acetate and ATP formation from acetyl-CoA I 2 2 1
tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate 2 2 1
glycine degradation (reductive Stickland reaction) 2 1 1
superpathway of 5-aminoimidazole ribonucleotide biosynthesis 6 6 2
pyruvate fermentation to acetate IV 3 3 1
pyruvate fermentation to acetate VII 3 3 1
superpathway of acetate utilization and formation 3 3 1
pyruvate fermentation to acetate II 3 3 1
pyruvate fermentation to acetate I 3 3 1
pyruvate fermentation to acetate and lactate II 4 3 1
pyruvate fermentation to acetate and (S)-lactate I 4 3 1
5-aminoimidazole ribonucleotide biosynthesis II 5 5 1
5-aminoimidazole ribonucleotide biosynthesis I 5 5 1
acetylene degradation (anaerobic) 5 2 1
ethanolamine utilization 5 2 1
methanogenesis from acetate 6 2 1
acetyl-CoA fermentation to butanoate 7 2 1
lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) 8 4 1
reductive glycine pathway of autotrophic CO2 fixation 9 7 1
superpathway of Clostridium acetobutylicum acidogenic fermentation 9 5 1
superpathway of fermentation (Chlamydomonas reinhardtii) 9 5 1
superpathway of L-alanine fermentation (Stickland reaction) 9 4 1
L-lysine fermentation to acetate and butanoate 10 3 1
gallate degradation III (anaerobic) 11 2 1
superpathway of tetrahydrofolate biosynthesis and salvage 12 10 1
(S)-lactate fermentation to propanoate, acetate and hydrogen 13 10 1
Bifidobacterium shunt 15 12 1
purine nucleobases degradation I (anaerobic) 15 5 1
mixed acid fermentation 16 11 1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 6 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 13 1
superpathway of purine nucleotides de novo biosynthesis I 21 18 1
superpathway of methanogenesis 21 2 1
superpathway of N-acetylneuraminate degradation 22 15 1
purine nucleobases degradation II (anaerobic) 24 12 1
superpathway of purine nucleotides de novo biosynthesis II 26 20 1
superpathway of L-lysine degradation 43 6 1
superpathway of histidine, purine, and pyrimidine biosynthesis 46 38 1