Experiment set2S493 for Bacteroides stercoris CC31F
MRS 1 x carbon source complex media
Group: carbon source complex mediaMedia: Varel_Bryant_medium + 1x MRS
Culturing: Bstercoris_CC31F_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/31/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
MRS 1x includes: 56 mM D-Glucose, 10 g/L Proteose Peptone, 10 g/L Beef extract, 5 g/L Yeast Extract, 1 g/L Tween 80, 0.2 g/L Triammonium citrate, 0.5 g/L Sodium acetate, 0.2 g/L Potassium phosphate dibasic, 0.1 g/L Magnesium sulfate, 0.05 g/L Manganese (II) sulfate monohydrate, 8.25 mM L-Cysteine
Specific Phenotypes
For 7 genes in this experiment
For carbon source complex media MRS in Bacteroides stercoris CC31F
For carbon source complex media MRS across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Respiratory Complex I | 5 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| NADH to cytochrome bd oxidase electron transfer I | 2 | 1 | 1 |
| NADH to cytochrome bo oxidase electron transfer I | 2 | 1 | 1 |
| aerobic respiration III (alternative oxidase pathway) | 3 | 2 | 1 |
| aerobic respiration I (cytochrome c) | 4 | 2 | 1 |
| NAD(P)/NADPH interconversion | 6 | 2 | 1 |
| Fe(II) oxidation | 6 | 1 | 1 |