Experiment set2S491 for Bacteroides stercoris CC31F
marine_broth_2216 1 x carbon source complex media
Group: carbon source complex mediaMedia: Varel_Bryant_medium + 1x marine_broth_2216
Culturing: Bstercoris_CC31F_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/31/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
marine_broth_2216 1x includes: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Specific Phenotypes
For 4 genes in this experiment
For carbon source complex media marine_broth_2216 in Bacteroides stercoris CC31F
For carbon source complex media marine_broth_2216 across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis | 1 |
| Menaquinone and Phylloquinone Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fatty acid metabolism
- Ubiquinone and menaquinone biosynthesis
- Alanine and aspartate metabolism
- Cyanoamino acid metabolism
- Nitrogen metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: