Experiment set2S490 for Bacteroides stercoris CC31F
LB 1 x carbon source complex media
Group: carbon source complex mediaMedia: Varel_Bryant_medium + 1x LB
Culturing: Bstercoris_CC31F_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/31/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
LB 1x includes: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Specific Phenotypes
For 2 genes in this experiment
For carbon source complex media LB in Bacteroides stercoris CC31F
For carbon source complex media LB across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glutamate dehydrogenases | 1 |
| Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glutamate metabolism
- Arginine and proline metabolism
- Nitrogen metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-glutamate biosynthesis III | 1 | 1 | 1 |
| L-glutamate degradation I | 1 | 1 | 1 |
| L-alanine degradation II (to D-lactate) | 3 | 2 | 1 |
| ethene biosynthesis IV (engineered) | 3 | 1 | 1 |
| L-glutamate and L-glutamine biosynthesis | 7 | 5 | 1 |
| L-glutamate degradation XI (reductive Stickland reaction) | 7 | 2 | 1 |
| 4-aminobutanoate degradation V | 7 | 1 | 1 |
| L-glutamate degradation V (via hydroxyglutarate) | 10 | 2 | 1 |
| methylaspartate cycle | 19 | 10 | 1 |