Experiment set2S446 for Bacteroides ovatus ATCC 8483

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Peptone 2.5 mg/mL carbon source complex media

Group: carbon source complex media
Media: Varel_Bryant_medium + Bacto Peptone (2.5 mg/mL)
Culturing: Bovatus_ATCC8483_ML6, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/30/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 4 genes in this experiment

For carbon source complex media Bacto Peptone in Bacteroides ovatus ATCC 8483

For carbon source complex media Bacto Peptone across organisms

SEED Subsystems

Subsystem #Specific
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 2
Glutamate dehydrogenases 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamate biosynthesis III 1 1 1
L-asparagine degradation I 1 1 1
L-glutamate degradation I 1 1 1
L-alanine degradation II (to D-lactate) 3 2 1
L-asparagine degradation III (mammalian) 3 2 1
ethene biosynthesis IV (engineered) 3 1 1
superpathway of L-aspartate and L-asparagine biosynthesis 4 4 1
N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation 5 3 1
incomplete reductive TCA cycle 7 6 1
L-glutamate and L-glutamine biosynthesis 7 5 1
L-glutamate degradation XI (reductive Stickland reaction) 7 1 1
4-aminobutanoate degradation V 7 1 1
L-glutamate degradation V (via hydroxyglutarate) 10 1 1
reductive TCA cycle I 11 9 1
gluconeogenesis III 12 10 1
(S)-lactate fermentation to propanoate, acetate and hydrogen 13 10 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 8 1
methylaspartate cycle 19 11 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 21 1