Experiment set2S440 for Bacteroides ovatus ATCC 8483

ABB 1 x carbon source complex media

Group: carbon source complex media
Media: Varel_Bryant_medium + 1x ABB
Culturing: Bovatus_ATCC8483_ML6, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/30/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

ABB 1x includes: 16 g/L Peptic digest of animal tissue, 7 g/L Yeast Extract, 5 g/L Sodium Chloride, 1 g/L Starch, 1 g/L D-Glucose, 1 g/L Sodium pyruvate, 1 g/L L-Arginine, 0.5 g/L Sodium succinate dibasic hexahydrate, 0.4 g/L Sodium bicarbonate, 0.5 g/L L-Cysteine hydrochloride monohydrate, 0.5 g/L Ferric pyrophosphate, 0.005 g/L Hemin, 0.0005 g/L Menadione, 1 g/L Dithiothreitol, 0.5 g/L Sodium thioglycolate

Specific Phenotypes

For 1 genes in this experiment

For carbon source complex media ABB in Bacteroides ovatus ATCC 8483

For carbon source complex media ABB across organisms

Metabolic Maps

Color code by fitness: see overview map or list of maps.

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
tRNA-uridine 2-thiolation and selenation (bacteria)	11	2	1