Experiment set2S427 for Bacteroides ovatus ATCC 8483
D-Mannose 10 mM carbon source
Group: carbon sourceMedia: Varel_Bryant_medium + D-Mannose (10 mM)
Culturing: Bovatus_ATCC8483_ML6, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/30/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 3 genes in this experiment
For carbon source D-Mannose in Bacteroides ovatus ATCC 8483
For carbon source D-Mannose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Hydrogenases | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| hydrogen production III | 1 | 1 | 1 |
| hydrogen production VIII | 1 | 1 | 1 |
| superpathway of hydrogen production | 2 | 2 | 1 |
| hydrogen production VI | 2 | 1 | 1 |
| thiazole component of thiamine diphosphate biosynthesis I | 6 | 5 | 1 |
| superpathway of photosynthetic hydrogen production | 6 | 1 | 1 |
| superpathway of fermentation (Chlamydomonas reinhardtii) | 9 | 7 | 1 |
| superpathway of thiamine diphosphate biosynthesis I | 10 | 9 | 1 |
| L-glutamate degradation VII (to butanoate) | 12 | 4 | 1 |