Experiment set2S423 for Bacteroides ovatus ATCC 8483

Compare to:

GAM 1 x carbon source complex media

Group: carbon source complex media
Media: Varel_Bryant_medium + 1x GAM
Culturing: Bovatus_ATCC8483_ML6, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/30/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

GAM 1x includes: 10 g/L Bacto Peptone, 3 g/L Soya peptone, 10 g/L Proteose Peptone, 13.5 g/L Digested serum, 5 g/L Yeast Extract, 2.2 g/L Meat extract, 1.2 g/L Liver extract, 3 g/L D-Glucose, 2.5 g/L Potassium phosphate monobasic, 3 g/L Sodium Chloride, 5 g/L Starch, 0.3 g/L L-Cysteine hydrochloride monohydrate, 0.3 g/L Sodium thioglycolate, 0.005 g/L Hemin, 0.001 g/L Menadione

Specific Phenotypes

For 14 genes in this experiment

For carbon source complex media GAM in Bacteroides ovatus ATCC 8483

For carbon source complex media GAM across organisms

SEED Subsystems

Subsystem #Specific
Campylobacter Iron Metabolism 1
De Novo Purine Biosynthesis 1
Glycolysis and Gluconeogenesis 1
Multidrug Resistance Efflux Pumps 1
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon) 1
PnuC-like transporters 1
Thiamin biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
5-aminoimidazole ribonucleotide biosynthesis II 5 5 1
5-aminoimidazole ribonucleotide biosynthesis I 5 5 1
superpathway of 5-aminoimidazole ribonucleotide biosynthesis 6 6 1
sucrose biosynthesis I (from photosynthesis) 9 6 1
glycolysis II (from fructose 6-phosphate) 11 10 1
formaldehyde assimilation III (dihydroxyacetone cycle) 12 10 1
gluconeogenesis III 12 10 1
gluconeogenesis I 13 11 1
glycolysis I (from glucose 6-phosphate) 13 11 1
Calvin-Benson-Bassham cycle 13 9 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 14 1
oxygenic photosynthesis 17 9 1
superpathway of hexitol degradation (bacteria) 18 13 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 14 1
superpathway of anaerobic sucrose degradation 19 14 1
superpathway of purine nucleotides de novo biosynthesis I 21 21 1
superpathway of N-acetylneuraminate degradation 22 17 1
ethene biosynthesis V (engineered) 25 15 1
superpathway of purine nucleotides de novo biosynthesis II 26 23 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 18 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 12 1
1-butanol autotrophic biosynthesis (engineered) 27 13 1
superpathway of histidine, purine, and pyrimidine biosynthesis 46 42 1