Experiment set2S423 for Bacteroides ovatus ATCC 8483

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GAM 1 x carbon source complex media

Group: carbon source complex media
Media: Varel_Bryant_medium + GAM (1 x)
Culturing: Bovatus_ATCC8483_ML6, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/30/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 11 genes in this experiment

For carbon source complex media GAM in Bacteroides ovatus ATCC 8483

For carbon source complex media GAM across organisms

SEED Subsystems

Subsystem #Specific
Campylobacter Iron Metabolism 1
Glycolysis and Gluconeogenesis 1
Hydrogenases 1
PnuC-like transporters 1
Thiamin biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
hydrogen production VIII 1 1 1
hydrogen production III 1 1 1
biotin biosynthesis from 8-amino-7-oxononanoate I 4 4 2
superpathway of hydrogen production 2 2 1
biotin biosynthesis from 8-amino-7-oxononanoate II 4 3 2
hydrogen production VI 2 1 1
biotin biosynthesis from 8-amino-7-oxononanoate III 5 3 2
biotin biosynthesis II 6 4 2
thiazole component of thiamine diphosphate biosynthesis I 6 5 1
superpathway of photosynthetic hydrogen production 6 1 1
biotin biosynthesis I 15 13 2
superpathway of fermentation (Chlamydomonas reinhardtii) 9 7 1
sucrose biosynthesis I (from photosynthesis) 9 6 1
superpathway of thiamine diphosphate biosynthesis I 10 9 1
glycolysis II (from fructose 6-phosphate) 11 10 1
formaldehyde assimilation III (dihydroxyacetone cycle) 12 10 1
gluconeogenesis III 12 10 1
L-glutamate degradation VII (to butanoate) 12 4 1
glycolysis I (from glucose 6-phosphate) 13 11 1
gluconeogenesis I 13 11 1
Calvin-Benson-Bassham cycle 13 9 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 14 1
oxygenic photosynthesis 17 9 1
superpathway of hexitol degradation (bacteria) 18 13 1
superpathway of anaerobic sucrose degradation 19 14 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 14 1
superpathway of N-acetylneuraminate degradation 22 17 1
ethene biosynthesis V (engineered) 25 15 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 18 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 12 1
1-butanol autotrophic biosynthesis (engineered) 27 13 1