Experiment set2S415 for Bacteroides ovatus ATCC 8483

Tara gum 2.5 mg/ml carbon source

Group: carbon source
Media: Varel_Bryant_medium + Tara gum (2.5 mg/mL)
Culturing: Bovatus_ATCC8483_ML6, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/30/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 7 genes in this experiment

For carbon source Tara gum in Bacteroides ovatus ATCC 8483

For carbon source Tara gum across organisms

SEED Subsystems

Subsystem	#Specific
Bacterial hemoglobins	1
Fructooligosaccharides(FOS) and Raffinose Utilization	1
Galactosylceramide and Sulfatide metabolism	1
Iron acquisition in Vibrio	1
Lactose and Galactose Uptake and Utilization	1
Melibiose Utilization	1
Predicted carbohydrate hydrolases	1
Sialic Acid Metabolism	1
Ton and Tol transport systems	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Galactose metabolism
• Nucleotide sugars metabolism
• Aminosugars metabolism
• Glycerolipid metabolism
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - globo series

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
melibiose degradation	1	1	1
N-acetylneuraminate and N-acetylmannosamine degradation II	3	2	1
β-(1,4)-mannan degradation	7	6	2
stachyose degradation	7	4	2